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Biologia Plantarum

, Volume 52, Issue 4, pp 718–722 | Cite as

Specific activation of PR-10 pathogenesis-related genes in apple by an incompatible race of Venturia inaequalis

  • M. Chevalier
  • L. Parisi
  • B. Gueye
  • C. Campion
  • P. Simoneau
  • P. Poupard
Brief Communication

Abstract

Apple (Malus × domestica Borkh. cv. Golden Delicious) trees were inoculated with fungal pathogens, corresponding either to an incompatible strain of Venturia inaequalis or to non-host pathogens (Venturia pyrina, Alternaria brassicicola) in order to characterize the regulation of PR-10 genes in these different situations in relationship to symptom development. Macro-and microscopic observations of the plant-fungus interactions revealed typical symptoms of resistance with V. inaequalis and symptoms of hypersensitivity (HR) on around 5 % of leaves with V. pyrina. No HR was microscopically observed with A. brassicicola. In the non-host situations, the PR-10 expression in leaves at the transcriptional level was not (A. brassicicola) or very slightly (V. pyrina) activated. This strongly suggests that PR-10 is not a component of the non-host resistance in the interactions studied here. By contrast specific up-regulation of PR-10 was evident after inoculation with V. inaequalis.

Additional key words

Alternaria brassicicola resistance mechanisms Malus × domestica Venturia pyrina 

Abbreviations

Ct

cycle threshold

HR

hypersensitive response

ITS

internal transcribed spacers

PR

pathogenesis related

SEM

scanning electron microscopy

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • M. Chevalier
    • 1
  • L. Parisi
    • 2
  • B. Gueye
    • 3
  • C. Campion
    • 3
  • P. Simoneau
    • 3
  • P. Poupard
    • 3
  1. 1.UMR GENHORT 1259, Unité d’Amélioration des Espèces Fruitières et OrnementalesCentre INRA d’AngersBeaucouzé CedexFrance
  2. 2.UMR PAVE 77, Unité de Pathologie VégétaleCentre INRA d’AngersBeaucouzé CedexFrance
  3. 3.UFR SciencesUniversité d’AngersAngers Cedex 01France

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