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Specific activation of PR-10 pathogenesis-related genes in apple by an incompatible race of Venturia inaequalis

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

Apple (Malus × domestica Borkh. cv. Golden Delicious) trees were inoculated with fungal pathogens, corresponding either to an incompatible strain of Venturia inaequalis or to non-host pathogens (Venturia pyrina, Alternaria brassicicola) in order to characterize the regulation of PR-10 genes in these different situations in relationship to symptom development. Macro-and microscopic observations of the plant-fungus interactions revealed typical symptoms of resistance with V. inaequalis and symptoms of hypersensitivity (HR) on around 5 % of leaves with V. pyrina. No HR was microscopically observed with A. brassicicola. In the non-host situations, the PR-10 expression in leaves at the transcriptional level was not (A. brassicicola) or very slightly (V. pyrina) activated. This strongly suggests that PR-10 is not a component of the non-host resistance in the interactions studied here. By contrast specific up-regulation of PR-10 was evident after inoculation with V. inaequalis.

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Abbreviations

Ct:

cycle threshold

HR:

hypersensitive response

ITS:

internal transcribed spacers

PR:

pathogenesis related

SEM:

scanning electron microscopy

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Authors and Affiliations

  1. UMR GENHORT 1259, Unité d’Amélioration des Espèces Fruitières et Ornementales, Centre INRA d’Angers, 42 rue G. Morel, BP 57, F-49071, Beaucouzé Cedex, France

    M. Chevalier

  2. UMR PAVE 77, Unité de Pathologie Végétale, Centre INRA d’Angers, 42 rue G. Morel, BP 57, F-49071, Beaucouzé Cedex, France

    L. Parisi

  3. UFR Sciences, Université d’Angers, 2 boulevard Lavoisier, F-49045, Angers Cedex 01, France

    B. Gueye, C. Campion, P. Simoneau & P. Poupard

Authors
  1. M. Chevalier
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  2. L. Parisi
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  3. B. Gueye
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  4. C. Campion
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  5. P. Simoneau
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  6. P. Poupard
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Correspondence to P. Poupard.

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Chevalier, M., Parisi, L., Gueye, B. et al. Specific activation of PR-10 pathogenesis-related genes in apple by an incompatible race of Venturia inaequalis . Biol Plant 52, 718–722 (2008). https://doi.org/10.1007/s10535-008-0138-9

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  • DOI: https://doi.org/10.1007/s10535-008-0138-9

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