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Biologia Plantarum

, 52:660 | Cite as

Dormancy and germination in wheat embryos: ribonucleases and hormonal control

  • C. Spanò
  • R. Buselli
  • I. Grilli
Original Papers

Abstract

Acidic and neutral ribonucleases (RNases) were studied in embryos of Triticum durum cv. Cappelli and the effects of abscisic acid (ABA) and gibberellic acid (GA3) were analysed. RNases activities increased during germination and were comparable in dormant and non-dormant embryos imbibed for 24 h. ABA generally inhibited ribonucleolytic activities, while GA3 only affected dormant embryos. To assess whether changes in RNase activities during germination or following hormonal treatment required new transcriptional or translational action, cycloheximide or cordycepin were used. The action of inhibitors of acidic RNase activity was found only in non-dormant-embryos. Findings obtained in the present work concur with a change of the ribonucleolytic pattern in the shift from dormant to non dormant metabolism.

Additional key words

abscisic acid cordycepin cycloheximide gibberellic acid 

Abbreviations

ABA

abscisic acid

A-RNases

acidic Rnases

B-RNases

neutral salt-stimulated Rnases

CHX

cycloheximide

Cor

cordycepin

C-RNases

neutral salt-inhibited Rnases

D

dormant

GA3

gibberellic acid

GM

germination medium

ND

non dormant

SDS-PAGE

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Dipartimento di BiologiaUniversità di PisaPisaItaly

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