Abstract
The in vitro response of sweet cherry (Prunus cerasus × P. canescens) rootstock Gisela 5 to increasing water deficit in the culture medium was studied. Water stress induced by the incorporation of 1, 2 and 4 % polyethylene glycol (PEG-8000) into the Murashige and Skoog medium was applied for 6 weeks. PEG-induced water stress reduced shoot dry mass, length, water content and relative chlorophyll content. Water stress also induced leaf necrosis without causing loss of viability in the explants. The increase in malondialdehyde content indicated oxidative stress. The activities of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POX) and glutathione reductase (GR) were also significantly elevated. The concentrations of K, Ca, Fe and Mn of shoots were decreased.
Abbreviations
- APX:
-
ascorbate peroxidase
- CAT:
-
catalase
- Chl:
-
chlororphyll
- GR:
-
glutathione reductase
- PEG:
-
polyethylene glycol
- POX:
-
peroxidase
- SOD:
-
superoxide dismutase
- WC:
-
water content
References
Al-Khayri, J.M., Al-Bahrany, A.M.: Growth, water content and proline accumulation in drought stressed callus of date palm.-Biol. Plant. 48: 105–108, 2004.
Bates, L.S., Waldren, R.P., Teare, I.D.: Rapid determination of free proline for water-stress studies.-Plant Soil 39: 205–207, 1973.
Beauchamp, C., Fridovich, I.: Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.-Anal. Biochem. 44: 276–287; 1971.
Bergmeyer, N.: Methoden der Enzymatischen Analyse. Vol. 1. Akademie-Verlag, Berlin 1970.
Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.
Brito, G., Costa, A., Fonseca, H.M.A.C., Santos, C.V.: Response of Olea europaea ssp. maderensis in vitro shoots exposed to osmotic stress.-Sci. Hort. 97: 411–417, 2003.
Cakmak, I.: The role of potassium in alleviating detrimental effects of abiotic stresses in plants.-J. Plant Nutr. Soil Sci. 168: 521–530, 2005.
Chai, T.T., Fadzillah, N.M., Kusnan, M., Mahmoodi, M.: Water stress induced oxidative damage and antioxidant responses in micropropagated banana plantlets.-Biol. Plant. 49: 153–156, 2005.
Dami, I., Hughes, H.: Leaf anatomy and water loss of in vitro PEG-treated ‘Valiant’ grape.-Plant Cell Tissue Organ Cult. 42: 179–184, 1995.
Erturk, U., Sivritepe, N., Yerlikaya, C., Bor, M., Özdemir, F., Turkan, I.: Responses of the cherry rootstock to salinity in vitro.-Biol. Plant. 51: 597–600, 2007.
Foyer, C.H., Halliwell, B.: Presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism.-Planta 133: 21–25, 1976.
Hernandez-Sebastia C., Samson, G., Bernier, P.Y., Piche, Y., Desjardins, Y.: Glomus intraradices causes differential changes in amino acid and starch concentrations of in vitro strawberry subjected to water stress.-New Phytol. 148: 177–186, 2000.
Herzog, V., Fahimi, H.: Determination of the activity of peroxidase.-Anal. Biochem. 55: 554–562, 1973.
Jain, M., Nandwal, A.S., Kundu, B.S., Kumar, B., Sheoran, I.S., Kumar, N., Mann, A., Kukreja, S.: Water relations, activities of antioxidants, ethylene evolution and membrane integrity of pigeonpea roots as affected by soil moisture.-Biol. Plant. 50: 303–306, 2006.
Katerji N., Hoom, J.W., Hamdy, A., Mastrorilli, M., Karzel, E.M.: Osmotic adjustment of sugar beets in response to soil salinity and its influence on stomatal conductance, growth and yield.-Agr. Water Manage. 34: 57–69. 1997.
Madhava K.V.R., Sresty, T.V.S.: Antioxidative parameters in the seedlings of pigeon pea (Cajanus cajan L. Millspaugh) in response to Zn and Ni stresses.-Plant Sci. 157: 113–128, 2000.
Molassiotis, A.N., Sotiropoulos, T., Tanou, G., Kofidis, G., Diamantidis, G., Therios, I.: Antioxidant and anatomical responses in shoot culture of the apple rootstock MM 106 treated with NaCl, KCl, mannitol or sorbitol.-Biol. Plant. 50: 61–68, 2006.
Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts.-Plant Cell Physiol. 22: 867–880, 1981.
Patakas, A., Nikolaou, N., Zioziou, E., Radoglou, K., Noitsakis, B.: The role of organic solute and ion accumulation in osmotic adjustment in drought-stressed grapevines.-Plant Sci. 163: 361–367, 2002.
Radic, S., Radic-Stojkovic, M., Kozlina-Pevalek, B. Influence of NaCl and mannitol on peroxidase activity and lipid peoxidation in Centaurea ragusina L. roots and shoots.-J. Plant Physiol. 163: 1284–1292, 2006.
Sawwan, J., Shibli, R.A., Swaidat, I., Tahat, M.: Phosphorus regulates osmotic potential and growth of African violet under in vitro-induced water deficit.-J. Plant Nutr. 23: 759–771, 2000.
Sotiropoulos, T.E.: Effect of NaCl and CaCl2 on growth and contents of minerals, chlorophyll, proline and sugars in the apple rootstock M 4 cultured in vitro.-Biol. Plant. 51: 177–180, 2007.
Sotiropoulos, T.E., Molassiotis, A., Almaliotis, D., Mouhtaridou, G., Dimassi, K., Therios, I., Diamantidis, G.: Growth, nutritional status, chlorophyll content, and antioxidant responses of the apple rootstock MM 111 shoots cultured under high boron concentrations in vitro.-J. Plant Nutr. 29: 575–583, 2006.
Zlatev, Z.S., Lidon, F.C., Ramalho, J.C., Yordanov, I.T.: Comparison of resistance to drought of three bean cultivars.-Biol. Plant. 50: 389–394, 2006.
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Sivritepe, N., Erturk, U., Yerlikaya, C. et al. Response of the cherry rootstock to water stress induced in vitro . Biol Plant 52, 573–576 (2008). https://doi.org/10.1007/s10535-008-0114-4
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DOI: https://doi.org/10.1007/s10535-008-0114-4