Biologia Plantarum

, 52:307 | Cite as

Water-water cycle involved in dissipation of excess photon energy in phosphorus deficient rice leaves

Original Papers


The water-water cycle which may be helpful for dissipating the excitation pressure over electron transport chain and minimizing the risk of photoinhibition and photodamage was investigated in rice after 10-d P-deficient treatment. Net photosynthetic rate decreased under P-deficiency, thus the absorption of photon energy exceeded the energy required for CO2 assimilation. A more sensitive response of effective quantum yield of photosystem 2 (ΦPS2) to O2 concentration was observed in plants that suffered P starvation, indicating that more electrons were transported to O2 in the P-deficient leaves. The electron transport rate through photosystem 2 (PS 2) (Jf) was stable, and the fraction of electron transport rate required to sustain CO2 assimilation and photorespiration (Jg/Jf) was significantly decreased accompanied by an increase in the alternative electron transport (Ja/Jf), indicating that a considerable electron amount had been transported to O2 during the water-water cycle in the P-deficient leaves. However, the fraction of electron transport to photorespiration (Jo/Jf) was also increased in the P-deficient leaves and it was less sensitive than that of water-water cycle. Therefore, water-water cycle could serve as an efficient electron sink. The higher non-photochemical fluorescence quenching (qN) in the P-deficient leaves depended on O2 concentration, suggesting that the water-water cycle might also contribute to non-radiative energy dissipation. Hence, the enhanced activity of the water-water cycle is important for protecting photosynthetic apparatus under P-deficiency in rice.

Additional key words

Oryza sativa net photosynthetic rate stomatal conductance intercellular CO2 concentration photosystem 2 chlorophyll a fluorescence non-photochemical and photochemical quenching photorespiration 



ascorbate peroxidase


intercellular CO2 concentration




stomatal conductance


fresh mass


the rate of alternative electron transport


the electron transport rate through PS2


the rate of electron transport required to maintain photosynthetic carbon reduction cycle (PCR) and photorespiratory carbon oxidation cycle (PCO)


the rate of electron transport though photorespiration




superoxide radical


net photosynthetic rate


photorespiratory carbon oxidation cycle


photosynthetic carbon reduction cycle


photosynthetic photon flux density



PS 2

photosystem 2


photochemical quenching


non-photochemical quenching


superoxide dismutase




trichloroacetic acid


effective PS2 quantum yield


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Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2008

Authors and Affiliations

  • X. -Y. Weng
    • 1
  • H. -X. Xu
    • 1
  • Y. Yang
    • 1
  • H. -H. Peng
    • 1
  1. 1.National Laboratory of Plant Physiology and Biochemistry, Department of Biological Science, College of Life ScienceZhejiang UniversityHangzhou, ZhejiangChina

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