Biologia Plantarum

, Volume 52, Issue 1, pp 26–35 | Cite as

Agrobacterium rhizogenes mediated transformation of Scutellaria baicalensis and production of flavonoids in hairy roots

  • R. K. Tiwari
  • M. Trivedi
  • Z.-C. Guang
  • G.-Q. Guo
  • G.-C. Zheng
Original Papers


Using different explants of in vitro seed grown Scutellaria baicalensis Georgi plantlets, hairy roots were induced following inoculation of Agrobacterium rhizogenes strains A4GUS, R1000 LBA 9402 and ATCC11325. The A4GUS proved to be more competent than other strains and the highest transformation rates were observed in cotyledonary leaf explant (42.6 %). The transformed roots appeared after 15–20 d of incubation on hormone free Murashige and Skoog medium. Growth of hairy roots was assessed on the basis of total root elongation, lateral root density and biomass accumulation. Maximum growth rate was recorded in root:medium ratio 1:100 (m/v). Hairy root lines were further established in Gamborg B5 medium and the biomass increase was maximum from 15 to 30 d. PCR, Southern hybridization and RT-PCR confirmed integration and expression of left and right termini-linked Ri T-DNA fragment of the Ri plasmid from A4GUS into the genome of Scutellaria baicalensis hairy roots. GUS assay was also performed for further integration and expression. All the clones showed higher growth rate them non-transformed root and accumulated considerable amounts of the root-specific flavonoids. Baicalin content was 14.1–30.0 % of dry root mass which was significantly higher then that of control field grown roots (18 %). The wogonin content varies from 0.08 to 0.18 % among the hairy root clones which was also higher than in non-transformed roots (0.07 %).

Additional key words

baicalin genetic transformation medicinal plants wogonin 



agropine synthase

B5 medium

Gamborg B5 medium


fresh mass


high performance liquid chromatography

MS medium

Murashige and Skoog medium




polymerase chain reaction


root-inducing plasmid


left-terminus DNA


right-terminus DNA


yeast extract broth


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Copyright information

© Institute of Experimental Botany, ASCR 2008

Authors and Affiliations

  1. 1.Institute of Cell Biology, School of life SciencesLanzhou UniversityLanzhouP.R. China

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