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Phylogenetic relationships among the bamboo species as revealed by morphological characters and polymorphism analyses

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Biologia Plantarum

Abstract

Phylogenetic relationships among 15 bamboo species were evaluated using 32 key morphological descriptors and 120 polymorphic loci of the genomic DNA generated using randomly amplified polymorphic DNA (RAPD) technique. The dendrogram generated for these two parameters based on similarity matrix computed from allelic polymorphism data using unweighted pair group method of analysis. Phylogenetic relationships as revealed from the dendrogram and principal component analysis was in concurrence with the reliable, widely referred system of bamboo classification, while, the cluster pattern generated from the similarity matrix derived from key morphological character analysis was discriminatory. The findings suggest that the molecular evidences need to be supplemented by morphological data to validate the phylogenetic relationships among taxa.

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Abbreviations

OTU:

operational taxonomic unit

PCA:

principal component analysis

PIC:

polymorphism information content

RAPD:

random amplified polymorphic DNA

UPGMA:

unweighted pair group method of arithmetic averages

References

  • Basak, J., Das, M., Ghose, T.K., Pal, A.: An efficient and high-yielding method of PCR-compatible DNA isolation for biotechnological investigations from economically important plants.-In: D’Souza, L., Anuradha, M., Shashikiran, N., Hegde, S., Rajendra, K. (ed.): Biotechnology for a Better Future. Pp. 99–105. SAC Publications, Mangalore 2004.

    Google Scholar 

  • Bennet, S.S.R., Gaur, R.C.: Thirty-seven Bamboos Growing in India.-Controller of Publications, Government of India, New Delhi 1990.

    Google Scholar 

  • Brown-Guedira, G.L., Thompson, J.A., Nelson, R.L., Warburton, M.L.: Evaluation of genetic diversity of soybean introductions and North American ancestors using RAPD and SSR markers.-Crop Sci. 40: 815–823, 2000.

    Article  CAS  Google Scholar 

  • Das, M., Bhattacharya, S., Pal, A,: Generation and characterization of SCARs by cloning and sequencing of RAPD products: A strategy for species specific marker development in bamboo.-Ann. Bot. 95: 835–841, 2005.

    Article  PubMed  CAS  Google Scholar 

  • Ding, Y.: Systematic studies on Phyllostachys.-Ph. D. Diss. Nanjing University, Nanjing 1998.

    Google Scholar 

  • Friar, E., Kochert, G.: A study of genetic variation and evolution of Phyllostachys (Bambusoideae: Poaceae) using nuclear restriction fragment length polymorphisms.-Theor. appl. Genet. 89: 265–270, 1994.

    Article  Google Scholar 

  • Gamble, J.S.: Bambuseae of British India.-Ann. Roy. Bot. Garden, Calcutta 1896.

    Google Scholar 

  • Gielis, J., Everaert, I., De, L.M.: Analysis of genetic variability and relationships in Phyllostachys using random amplified polymorphic DNA.-In: Chapman, G. (ed.): The Bamboos. Pp.107–124. Academic Press, London 1997.

    Google Scholar 

  • Gunter, L.E., Tuskan, G.A., Wullschleger, S.D.: Diversity among populations of switchgrass based on RAPD markers.-Crop. Sci. 36: 1017–1022, 1996.

    Article  Google Scholar 

  • Guo, Z.H., Chen, Y.Y., Li, D.Z., Yang, J.B.: Genetic variation and evolution of the alpine bamboos (Poaceae, Bambusoideae) using DNA sequence data.-J. Plant Res. 114: 315–322, 2001.

    Article  CAS  Google Scholar 

  • Hodkinson, T.R., Renvoize, S.A., Chonghaile, G.N., Stapleton, C.M.A., Chase, M.W.: A comparison of ITS nuclear rDNA sequence data and AFLP markers for phylogenetic studies in Phyllostachys (Bambusoideae, Poaceae).-J. Plant Res. 113: 259–269, 2000.

    Article  CAS  Google Scholar 

  • Hsiao, J.Y., Rieseberg, L.H.: Population genetic structure of Yushania niitakayamensis (Bambusoideae, Poaceae) in Taiwan.-Mol. Ecol. 3: 201–208, 1994.

    Google Scholar 

  • Kobayashi, M.: Phylogeny of world bamboos analysed by restriction fragment length polymorphisms of chloroplast DNA.-In: Chapman, G.P. (ed.): The Bamboos. Pp. 227–234. Linnean Society, London 1997.

    Google Scholar 

  • Loh, J.P., Kiew, R., Set, O., Gan, L.H., Gan, Y.Y.: A study of genetic variation and relationships within the bamboo subtribe Bambusinae using amplified fragment length polymorphism.-Ann. Bot. 85: 607–612, 2000.

    Article  CAS  Google Scholar 

  • Nair, N.V., Nair, S., Sreenivasan, T.V., Mohan, M.: Analysis of genetic diversity and phylogeny in Saccharum and related genera using RAPD markers.-Genet. Res. Crop Evol. 46: 73–79, 1999.

    Article  Google Scholar 

  • Nayak, S., Rout, G.R., Das, P.: Evaluation of the genetic variability in bamboo using RAPD markers.-Plant Soil Environ. 49: 24–28, 2003.

    CAS  Google Scholar 

  • Nei, M.: Estimation of average heterozygosity and genetic distance from a small number of individuals.-Genetics 89: 583–590, 1978.

    PubMed  CAS  Google Scholar 

  • Nei, M., Li, W.: Mathematical model for studying genetic variation in terms of restriction endonucleases.-Proc. nat. Acad. Sci. USA 79: 5269–5273, 1979.

    Article  Google Scholar 

  • Ni, J., Colowit, P.M., Mackill, D.J.: Evaluation of genetic diversity in rice subspecies using microsatellite markers.-Crop Sci. 42: 601–607, 2002.

    Article  CAS  Google Scholar 

  • Ohrnberger, D., Goerrings, J.: The Bamboos of the World.-Elsevier Science, Amsterdam 2002.

    Google Scholar 

  • Padmesh, P., Reji, J.V., Jinish Dhar, M., Seeni, S.: Estimation of genetic diversity in varieties of Mucuna pruriens using RAPD.-Biol. Plant. 50: 367–372, 2006.

    Article  CAS  Google Scholar 

  • Raizada, M.B., Chatterjee, R.N.: World distribution of bamboos with special reference to the Indian species and their more important uses.-Indian Forest. 82: 215–218, 1956.

    Google Scholar 

  • Rohlf, F.J: NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System. Version 2.2. Exeter Software, Setauket-New York 2000.

    Google Scholar 

  • Rout, G.R.: Evaluation of genetic relationship in Typhonium species through random amplified polymorphic DNA markers.-Biol. Plant. 50: 127–130, 2006.

    Article  CAS  Google Scholar 

  • Sneath, P.H.A., Sokal, R.R.: Numerical Taxonomy.-W.H. Freeman and Co., San Francisco 1973.

    Google Scholar 

  • Weir, B.: Genetic Data Analysis: Methods for Discrete Population Genetic Data.-Sinauer Assoc., Sunderland 1990.

    Google Scholar 

  • Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V.: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.-Nucl. Acids Res. 18: 6531–6535, 1990.

    Article  PubMed  CAS  Google Scholar 

  • Wu, M.C.Y.: Classification of Bambuseae based on leaf anatomy.-Bot. Bull. Acad. sin. 3: 83–107, 1962.

    Google Scholar 

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Correspondence to A. Pal.

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Das, M., Bhattacharya, S., Basak, J. et al. Phylogenetic relationships among the bamboo species as revealed by morphological characters and polymorphism analyses. Biol Plant 51, 667–672 (2007). https://doi.org/10.1007/s10535-007-0140-7

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  • DOI: https://doi.org/10.1007/s10535-007-0140-7

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