Advertisement

Biologia Plantarum

, Volume 51, Issue 4, pp 667–672 | Cite as

Phylogenetic relationships among the bamboo species as revealed by morphological characters and polymorphism analyses

  • M. Das
  • S. Bhattacharya
  • J. Basak
  • A. Pal
Original Papers

Abstract

Phylogenetic relationships among 15 bamboo species were evaluated using 32 key morphological descriptors and 120 polymorphic loci of the genomic DNA generated using randomly amplified polymorphic DNA (RAPD) technique. The dendrogram generated for these two parameters based on similarity matrix computed from allelic polymorphism data using unweighted pair group method of analysis. Phylogenetic relationships as revealed from the dendrogram and principal component analysis was in concurrence with the reliable, widely referred system of bamboo classification, while, the cluster pattern generated from the similarity matrix derived from key morphological character analysis was discriminatory. The findings suggest that the molecular evidences need to be supplemented by morphological data to validate the phylogenetic relationships among taxa.

Additional key words

cluster analyses culm culm-sheath RAPD 

Abbreviations

OTU

operational taxonomic unit

PCA

principal component analysis

PIC

polymorphism information content

RAPD

random amplified polymorphic DNA

UPGMA

unweighted pair group method of arithmetic averages

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Basak, J., Das, M., Ghose, T.K., Pal, A.: An efficient and high-yielding method of PCR-compatible DNA isolation for biotechnological investigations from economically important plants.-In: D’Souza, L., Anuradha, M., Shashikiran, N., Hegde, S., Rajendra, K. (ed.): Biotechnology for a Better Future. Pp. 99–105. SAC Publications, Mangalore 2004.Google Scholar
  2. Bennet, S.S.R., Gaur, R.C.: Thirty-seven Bamboos Growing in India.-Controller of Publications, Government of India, New Delhi 1990.Google Scholar
  3. Brown-Guedira, G.L., Thompson, J.A., Nelson, R.L., Warburton, M.L.: Evaluation of genetic diversity of soybean introductions and North American ancestors using RAPD and SSR markers.-Crop Sci. 40: 815–823, 2000.CrossRefGoogle Scholar
  4. Das, M., Bhattacharya, S., Pal, A,: Generation and characterization of SCARs by cloning and sequencing of RAPD products: A strategy for species specific marker development in bamboo.-Ann. Bot. 95: 835–841, 2005.PubMedCrossRefGoogle Scholar
  5. Ding, Y.: Systematic studies on Phyllostachys.-Ph. D. Diss. Nanjing University, Nanjing 1998.Google Scholar
  6. Friar, E., Kochert, G.: A study of genetic variation and evolution of Phyllostachys (Bambusoideae: Poaceae) using nuclear restriction fragment length polymorphisms.-Theor. appl. Genet. 89: 265–270, 1994.CrossRefGoogle Scholar
  7. Gamble, J.S.: Bambuseae of British India.-Ann. Roy. Bot. Garden, Calcutta 1896.Google Scholar
  8. Gielis, J., Everaert, I., De, L.M.: Analysis of genetic variability and relationships in Phyllostachys using random amplified polymorphic DNA.-In: Chapman, G. (ed.): The Bamboos. Pp.107–124. Academic Press, London 1997.Google Scholar
  9. Gunter, L.E., Tuskan, G.A., Wullschleger, S.D.: Diversity among populations of switchgrass based on RAPD markers.-Crop. Sci. 36: 1017–1022, 1996.CrossRefGoogle Scholar
  10. Guo, Z.H., Chen, Y.Y., Li, D.Z., Yang, J.B.: Genetic variation and evolution of the alpine bamboos (Poaceae, Bambusoideae) using DNA sequence data.-J. Plant Res. 114: 315–322, 2001.CrossRefGoogle Scholar
  11. Hodkinson, T.R., Renvoize, S.A., Chonghaile, G.N., Stapleton, C.M.A., Chase, M.W.: A comparison of ITS nuclear rDNA sequence data and AFLP markers for phylogenetic studies in Phyllostachys (Bambusoideae, Poaceae).-J. Plant Res. 113: 259–269, 2000.CrossRefGoogle Scholar
  12. Hsiao, J.Y., Rieseberg, L.H.: Population genetic structure of Yushania niitakayamensis (Bambusoideae, Poaceae) in Taiwan.-Mol. Ecol. 3: 201–208, 1994.Google Scholar
  13. Kobayashi, M.: Phylogeny of world bamboos analysed by restriction fragment length polymorphisms of chloroplast DNA.-In: Chapman, G.P. (ed.): The Bamboos. Pp. 227–234. Linnean Society, London 1997.Google Scholar
  14. Loh, J.P., Kiew, R., Set, O., Gan, L.H., Gan, Y.Y.: A study of genetic variation and relationships within the bamboo subtribe Bambusinae using amplified fragment length polymorphism.-Ann. Bot. 85: 607–612, 2000.CrossRefGoogle Scholar
  15. Nair, N.V., Nair, S., Sreenivasan, T.V., Mohan, M.: Analysis of genetic diversity and phylogeny in Saccharum and related genera using RAPD markers.-Genet. Res. Crop Evol. 46: 73–79, 1999.CrossRefGoogle Scholar
  16. Nayak, S., Rout, G.R., Das, P.: Evaluation of the genetic variability in bamboo using RAPD markers.-Plant Soil Environ. 49: 24–28, 2003.Google Scholar
  17. Nei, M.: Estimation of average heterozygosity and genetic distance from a small number of individuals.-Genetics 89: 583–590, 1978.PubMedGoogle Scholar
  18. Nei, M., Li, W.: Mathematical model for studying genetic variation in terms of restriction endonucleases.-Proc. nat. Acad. Sci. USA 79: 5269–5273, 1979.CrossRefGoogle Scholar
  19. Ni, J., Colowit, P.M., Mackill, D.J.: Evaluation of genetic diversity in rice subspecies using microsatellite markers.-Crop Sci. 42: 601–607, 2002.CrossRefGoogle Scholar
  20. Ohrnberger, D., Goerrings, J.: The Bamboos of the World.-Elsevier Science, Amsterdam 2002.Google Scholar
  21. Padmesh, P., Reji, J.V., Jinish Dhar, M., Seeni, S.: Estimation of genetic diversity in varieties of Mucuna pruriens using RAPD.-Biol. Plant. 50: 367–372, 2006.CrossRefGoogle Scholar
  22. Raizada, M.B., Chatterjee, R.N.: World distribution of bamboos with special reference to the Indian species and their more important uses.-Indian Forest. 82: 215–218, 1956.Google Scholar
  23. Rohlf, F.J: NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System. Version 2.2. Exeter Software, Setauket-New York 2000.Google Scholar
  24. Rout, G.R.: Evaluation of genetic relationship in Typhonium species through random amplified polymorphic DNA markers.-Biol. Plant. 50: 127–130, 2006.CrossRefGoogle Scholar
  25. Sneath, P.H.A., Sokal, R.R.: Numerical Taxonomy.-W.H. Freeman and Co., San Francisco 1973.Google Scholar
  26. Weir, B.: Genetic Data Analysis: Methods for Discrete Population Genetic Data.-Sinauer Assoc., Sunderland 1990.Google Scholar
  27. Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V.: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.-Nucl. Acids Res. 18: 6531–6535, 1990.PubMedCrossRefGoogle Scholar
  28. Wu, M.C.Y.: Classification of Bambuseae based on leaf anatomy.-Bot. Bull. Acad. sin. 3: 83–107, 1962.Google Scholar

Copyright information

© Institute of Experimental Botany, ASCR 2007

Authors and Affiliations

  1. 1.Plant Molecular and Cellular GeneticsBose InstituteKolkataIndia

Personalised recommendations