Biologia Plantarum

, Volume 51, Issue 3, pp 521–524 | Cite as

Direct plant regeneration from cucumber embryonal axis

  • A. Vasudevan
  • N. Selvaraj
  • A. Ganapathi
  • C. W. Choi
  • M. Manickavasagam
  • S. Kasthurirengan
Brief Communication


Embryonal axis explants from 2-d-old in vitro germinated seeds were used to induce multiple shoot production. The combination of 4.44 µM BA and 1.59 µM NAA in MS medium triggered the initiation of adventitious shoot buds. The explants with shoot buds produced maximum number of shoots (10.6 per explant) in MS medium supplemented with 4.44 µM BA and 0.065 mM L-glutamine in three successive transfers. The elongated shoots were rooted on MS medium with 4.92 µM IBA. Rooted plants were transferred to soil with a survival rate of 65 %.

Additional key words

adventitious shoot bud benzyladenine Cucumis sativusL-glutamine naphthalene acetic acid 





indolebutyric acid



MS medium

Murashige and Skoog medium


α-naphthalene acetic acid


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Copyright information

© Institute of Experimental Botany, ASCR 2007

Authors and Affiliations

  • A. Vasudevan
    • 1
  • N. Selvaraj
    • 3
  • A. Ganapathi
    • 2
  • C. W. Choi
    • 1
  • M. Manickavasagam
    • 2
  • S. Kasthurirengan
    • 2
  1. 1.Department of Biology and Medicinal SciencePai Chai UniversityDaejeonSouth Korea
  2. 2.Department of BiotechnologyBharathidasan University, TiruchirappalliTamil NaduIndia
  3. 3.Department of BotanyPeriyar E.V.R. College (Autonomous), TiruchirappalliTamil NaduIndia

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