Biologia Plantarum

, Volume 51, Issue 2, pp 386–390 | Cite as

Effect of osmotic stress and sodium nitroprusside pretreatment on proline metabolism of wheat seedlings

  • Y. Lei
  • C. Yin
  • J. Ren
  • C. Li
Brief Communication


Effect of osmotic stress and sodium nitroprusside (SNP, NO donor) pretreatment on growth and proline metabolism of wheat seedlings was investigated. Polyethylene glycol 6000 treatment for 2, 4 and 6 d could be termed as mild, moderate and severe stress, respectively, according to decrease in the relative water content. Severe osmotic stress significantly decreased the growth and photochemical efficiency, and increased proline content due to activation of its synthesis. 0.2 mM SNP pretreatment enhanced growth of wheat seedlings, increased variable to maximum fluorescence ratio (Fv/Fm) and fluorescence yield, while decreased proline content. However, 2 mM SNP retarded the seedlings growth and chlorophyll a fluorescence, and increased proline accumulation. Our results showed that NO might be involved in the regulation of osmotic stress in a concentration-dependent manner.

Additional key words

chlorophyll a fluorescence exogenous nitric oxide Triticum aestivum



abscisic acid




P5C synthetase


polyethylene glycol


relative water content


sodium nitroprusside


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Copyright information

© Institute of Experimental Botany, ASCR 2007

Authors and Affiliations

  1. 1.Chengdu Institute of BiologyChinese Academy of SciencesChengduP.R. China

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