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Two calcium mobilizing pathways implicated within abscisic acid-induced stomatal closing in Arabidopsis thaliana

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

The present study investigated whether, depending on the abscisic acid (ABA) concentration, phospholipase C (PLC) would be implicated within a Ca2+ mobilizing pathway that would regulate stomatal aperture under standard watering conditions. Among Al sensitive mutants the als1-1 mutant of Arabidopsis thaliana (L.) Heynh. (Columbia-4 ecotype) was selected for a pharmacological approach of stomatal closing in leaf epidermal peels induced by 3, 20 or 30 µM ABA. Comparison with the wild type (WT) revealed that, exclusively in the als1-1 mutant, the stomatal response to 3 or 20 µM ABA was inhibited by about 40 %, whereas the stomatal response to 30 µM ABA and the wilting response to drought were unaffected. In WT, the Ca2+ buffer EGTA and the PLC inhibitor, 1-[6-[[17β-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione (U73122), specifically inhibited by about 70 and 40 %, respectively, the response to 3 or 20 µM ABA, while the Ca2+ buffer 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) inhibited by about 70 % the response to 3, 20 or 30 µM ABA. EGTA, BAPTA and U73122 did not inhibit the part of the response to 3 or 20 µM ABA that was unaffected by the als1-1 mutation. Together, these results showed that ABA closes the stomata through two different Ca2+ mobilizing pathways. Since PLC could be indirectly deactivated in the als1-1 mutant, these results might suggest that, under sufficient water supply, PLC-mediated Ca2+ mobilization is needed for the regulation of stomatal aperture by endogenous ABA resting at concentrations below a drought-specific threshold value.

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Abbreviations

ABA:

abscisic acid

alr:

Al resistant

als:

Al sensitive

BAPTA:

1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid

DAG:

diacylglycerol

GP Ant-2:

pGlu-Gln-D-Trp-Phe-D-Trp-D-Trp-Met-NH2

G protein:

GTP-binding protein

IP3 :

inositol triphosphate

PI:

phosphoinositide

PLC:

phospholipase C

7TMS:

seven-transmembrane-span

U73122:

1-[6-[[17β-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione

U73343:

1-[6-[[17β-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-2,5-pyrrolidine-dione

WT:

wild type

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  1. Laboratoire des Echanges Membranaires et Signalisation, UMR 6191 CNRS-CEA-AIX-Marseille II, F-13108, Saint-Paul-lez-Durance, Cedex, France

    A. Cousson

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Cousson, A. Two calcium mobilizing pathways implicated within abscisic acid-induced stomatal closing in Arabidopsis thaliana . Biol Plant 51, 285–291 (2007). https://doi.org/10.1007/s10535-007-0057-1

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  • DOI: https://doi.org/10.1007/s10535-007-0057-1

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