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Effect of seed soaking with thiols on the antioxidant enzymes and photosystem activities in wheat subjected to water stress

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Biologia Plantarum

Abstract

Photosystem 1 and 2 and antioxidant enzyme activities were determined in wheat (Triticum aestivum L. cv. Sonalika) leaves. Seedlings from both control seeds and seeds soaked in solutions like dithiothreitol, thioglycollic acid and thiourea were subjected to water stress induced by polyethylene glycol. Photosystem 1 and 2 activities were less inhibited by water stress due to seed soaking with sulphydryl compounds. The changes in activities of antioxidant enzymes induced by water stress were higher in seedlings from thiol-pretreated seeds than from water-soaked seeds.

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Abbreviations

ε:

coefficient of absorbance

APOX:

ascorbate peroxidase

CAT:

catalase

Chl:

chlorophyll

DCQ:

2,6-dichloro-p-benzoquinone

DTT:

dithiothreitol

EDTA:

ethylenediaminetetraacetic acid

GPOX:

guaiacol peroxidase

GR:

glutathione reductase

GSH:

glutathione reduced form

GSSG:

glutathione oxidised form

GST:

glutathione-S-transferase

MeV:

methyl viologen

NADP+ :

nicotinamide adenine dinucleotide oxidised form

NADPH:

nicotinamide adenine dinucleotide phosphate reduced form

NBT:

nitroblue tetrazolium

PEG:

polyethylene glycol

PS 1, PS 2:

photosystems 1 and 2

ROS:

reactive oxygen species

PVP:

polyvinylpolypyrrolidone

SOD:

superoxide dismutase

TGA:

thioglycolic acid

TU:

thiourea

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Correspondence to N. K. Ramaswamy.

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Nathawat, N.S., Nair, J.S., Kumawat, S.M. et al. Effect of seed soaking with thiols on the antioxidant enzymes and photosystem activities in wheat subjected to water stress. Biol Plant 51, 93–97 (2007). https://doi.org/10.1007/s10535-007-0019-7

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  • DOI: https://doi.org/10.1007/s10535-007-0019-7

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