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Biologia Plantarum

, Volume 51, Issue 1, pp 93–97 | Cite as

Effect of seed soaking with thiols on the antioxidant enzymes and photosystem activities in wheat subjected to water stress

  • N. S. Nathawat
  • J. S. Nair
  • S. M. Kumawat
  • N. S. Yadava
  • G. Singh
  • N. K. Ramaswamy
  • M. P. Sahu
  • S. F. D’Souza
Original Papers

Abstract

Photosystem 1 and 2 and antioxidant enzyme activities were determined in wheat (Triticum aestivum L. cv. Sonalika) leaves. Seedlings from both control seeds and seeds soaked in solutions like dithiothreitol, thioglycollic acid and thiourea were subjected to water stress induced by polyethylene glycol. Photosystem 1 and 2 activities were less inhibited by water stress due to seed soaking with sulphydryl compounds. The changes in activities of antioxidant enzymes induced by water stress were higher in seedlings from thiol-pretreated seeds than from water-soaked seeds.

Additional key words

oxygen evolution peroxidase photosynthesis polyethylene glycol seed pretreatment sulphydryl compounds 

Abbreviations

ε

coefficient of absorbance

APOX

ascorbate peroxidase

CAT

catalase

Chl

chlorophyll

DCQ

2,6-dichloro-p-benzoquinone

DTT

dithiothreitol

EDTA

ethylenediaminetetraacetic acid

GPOX

guaiacol peroxidase

GR

glutathione reductase

GSH

glutathione reduced form

GSSG

glutathione oxidised form

GST

glutathione-S-transferase

MeV

methyl viologen

NADP+

nicotinamide adenine dinucleotide oxidised form

NADPH

nicotinamide adenine dinucleotide phosphate reduced form

NBT

nitroblue tetrazolium

PEG

polyethylene glycol

PS 1, PS 2

photosystems 1 and 2

ROS

reactive oxygen species

PVP

polyvinylpolypyrrolidone

SOD

superoxide dismutase

TGA

thioglycolic acid

TU

thiourea

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Copyright information

© Institute of Experimental Botany, ASCR 2007

Authors and Affiliations

  • N. S. Nathawat
    • 1
  • J. S. Nair
    • 2
  • S. M. Kumawat
    • 1
  • N. S. Yadava
    • 1
  • G. Singh
    • 1
  • N. K. Ramaswamy
    • 2
  • M. P. Sahu
    • 1
  • S. F. D’Souza
    • 2
  1. 1.Rajasthan Agricultural UniversityBikanerIndia
  2. 2.Nuclear Agriculture and Biotechnology DivisionBhabha Atomic Research CentreMumbaiIndia

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