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Biologia Plantarum

, Volume 50, Issue 4, pp 775–778 | Cite as

Nitric oxide treatment alleviates drought stress in wheat seedlings

  • X. Tian
  • Y. Lei
Brief Communication

Abstract

The effects of sodium nitroprusside (SNP; nitric oxide donor) treatment on drought stress induced by PEG for different periods of time in wheat seedlings were investigated. Our results suggested that treatment for 2, 4 and 6 d with 15 % PEG could be termed as mild, moderate and severe stress, respectively. Drought stress induced accumulation of hydrogen peroxide and resulted in lipid peroxidation. On the other hand, activities of SOD, CAT and PAL increased under mild stress to counteract the oxidative injury and then decreased when the stress became severe (6 d). As the effect of SNP treatment, 0.2 mM enhanced wheat seedlings growth and kept high relative water content and alleviated the oxidative damage. However, 2 mM SNP aggravated the stress as a result of uncontrolled generation of reactive oxygen species and ineffectiveness of antioxidant systems.

Additional key words

ascorbate peroxidase catalase guaiacol peroxidase L-phenylalanine ammonia lyase reactive oxygen species superoxide dismutase Triticum aeslivum 

Abbreviations

AsA

ascorbic acid

APX

ascorbate peroxidase

CAT

catalase

EDTA

ethylene diamine tetraacetic acid

GPX

guaiacol peroxidase

H2O2

hydrogen peroxide

PAL

L-phenylalanine ammonia lyase

ROS

reactive oxygen species

RWC

relative water content

SNP

sodium nitroprusside

SOD

superoxide dimutase

TBARS

thiobarburic acid reacting substance

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References

  1. Aebi, H.E.: Catalase.-In: Bergmeyer, H.U. (ed.): Methods of Enzymatic Analysis. Vol. 3. Pp. 273–282. Verlag-Chemie, Weinheim 1983.Google Scholar
  2. Asada, K.: Production and action of active oxygen species in photosynthetic tissues.-In: Foyer, C.H., Mullineaux, P.M. (ed.): Causes of Photooxidative Stress and Amelioration of Defense System in Plants. Pp. 77–103, CRC Press, Boca Raton 1994.Google Scholar
  3. Baisak, R., Rana, D., Acharya, P.B.B., Kar, M.: Alterations in activities of oxygen active scavenging enzymes of wheat leaves subjected to water stress.-Plant Cell Physiol. 35: 489–495, 1994.Google Scholar
  4. Bajaj, S., Jayaprakash, T., Li, L., Ho, T.H.D., Wu, R.: Transgenic approaches to increase dehydration-stress tolerance in plants.-Mol. Breed. 5: 493–503, 1999.CrossRefGoogle Scholar
  5. Beligni, M.V., Lamattina, L.: Nitric oxide counteracts cytotoxic processes mediated by reactive oxygen species in plant tissues.-Planta 208: 337–344, 1999.CrossRefGoogle Scholar
  6. Bradford, M.M.: A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.PubMedCrossRefGoogle Scholar
  7. Brennan, T., Frenkel, C.: Involvement of hydrogen peroxide in the regulation of senescence in pear.-Plant Physiol. 59: 411–416, 1977.PubMedGoogle Scholar
  8. Chaparzadeh, N., D’Amico, M.L., Khavari-Nejad, R.A., Izzo, R., Navari-Izzo, F.: Antioxidant responses of Calendula officinalis under salinity conditions.-Plant Physiol. Biochem. 42: 695–701, 2004.PubMedCrossRefGoogle Scholar
  9. Conner, E.M., Grisham, M.B.: Inflammation, free radicals and antioxidants.-Nutrition 12: 274–277, 1996.PubMedCrossRefGoogle Scholar
  10. D’Cunha, G.B., Satyanarayan, V., Nair, P.M.: Purification of phenylalanine ammonia-lyase from Rhodotorula glutinis.-Phytochemistry 42: 17–20, 1996.CrossRefGoogle Scholar
  11. Frank, S., Kämpfer, H., Podda, M.: Identification of copper/zinc superoxide dismutase as a nitric oxide-regulated gene in human (HaCaT) keratinocytes: implications for keratinocyte proliferation.-Biochem. J. 346: 719–728, 2000.PubMedCrossRefGoogle Scholar
  12. Giannopolitis, C.N., Ries, S.K.: Superoxide dismutase I: Occurrence in higher plants.-Plant Physiol. 77: 309–314, 1977.CrossRefGoogle Scholar
  13. Haslam, E.: Practical Polyphenolics: From Structure to Molecular Recognition and Physiological Action.-Cambridge University Press, Cambridge 1998.Google Scholar
  14. Hodges, D.M., DeLong, J.M., Forney, C.F., Prange, R.K.: Improving the thiobarbituric acid-reactive-substance assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds.-Planta 207: 604–611, 1999.CrossRefGoogle Scholar
  15. Hsiao, T.C.: Plant response to water stress.-Annu. Rev. Plant Physiol. 24: 519–570, 1973.CrossRefGoogle Scholar
  16. Huang, M., Guo, N.: Responses of antioxidative system to chilling stress in two rice cultivars differing in sensitivity.-Biol. Plant. 49: 81–84, 2005.CrossRefGoogle Scholar
  17. Lamotte, O., Gould, K., Lecourieux, D., Sequeira-Legrand, A., Lebun-Garcia, A., Durner, J., Pugin, A., Wendehenne, D.: Analysis of nitric oxide signaling functions in tobacco cells challenged by the elicitor cryptogein.-Plant Physiol. 135: 516–529, 2004.PubMedCrossRefGoogle Scholar
  18. Lin, J.S., Wang, G.X.: Doubled CO2 could improve the drought tolerance better in sensitive cultivars than in tolerant cultivars in spring wheat.-Plant Sci. 163: 627–637, 2002.CrossRefGoogle Scholar
  19. Smirnoff, N.: Plant resistance to environmental stress.-Curr. Opin. Biotechnol. 9: 214–219, 1998.PubMedCrossRefGoogle Scholar
  20. Tu, J., Shen, W.B., Xu, L.L.: Regulation of nitric acid on the aging process of wheat leaves.-Acta bot. sin. 45: 1055–1062, 2003.Google Scholar

Copyright information

© Institute of Experimental Botany, ASCR, Praha 2006

Authors and Affiliations

  1. 1.School of Biological Resources and Environmental SciencesJishou UniversityHunanP.R. China
  2. 2.Chengdu Institute of BiologyChinese Academy of SciencesChengduP.R. China

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