Biologia Plantarum

, Volume 50, Issue 4, pp 537–541 | Cite as

Regulations of granule-bound starch synthase I gene expression in rice leaves by temperature and drought stress

Original Paper


Effects of temperature (15/10, 25/20, 30/25, and 35/30 °C) and drought stresses on the expression of granule-bound starch synthase I (GBSSI) gene were examined in rice (Oryza sativa L.) seedlings. The GBSSI expression was higher at the low temperature (15/10 °C), and the transcript level decreased at temperatures higher than 30 °C. Protein phosphorylation was involved in the low temperature-stimulated signal transduction of GBSSI regulation. The expression of GBSSI in rice seedling was reduced under a drought stress. Even though exogenous ABA played a role to reduce the GBSSI transcript accumulation under non-stress condition, the reducing of GBSSI expression by drought stress appeared to be mediated by an ABA-independent pathway.

Additional key words

abscisic acid Oryza sativa 



abscisic acid




granule-bound starch synthase I


soluble starch synthase


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  1. Asaoka, M., Kazutoshi, O., Fuwa, H.: Effect of environmental temperature at the milky stage on amylose content and fine structure of amylopectin of waxy and nonwaxy endosperm starches of rice (Oryza sativa L.).-Agr. biol. Chem. 49: 373–379, 1985.Google Scholar
  2. Ashraf, M., Hafeez, M.: Thermotolerance of pearl millet and maize at early growth stages: growth and nutrient relations.-Biol. Plant. 48: 81–86, 2004.CrossRefGoogle Scholar
  3. Cao, H., James, M.G., Myers, A.M.: Purification and characterization of soluble starch synthases from maize.-Arch. Biochem. Biophys. 373: 135–146, 2000.PubMedCrossRefGoogle Scholar
  4. Chinnusamy, V., Khanna-Chopra, R.: Effect of heat stress on grain starch content in diploid, tetraploid and hexaploid wheat species.-J. Agron. Crop Sci. 189: 242–249, 2003.CrossRefGoogle Scholar
  5. Chu, C., Lee T.M.: The relationship between ethylene biosynthesis and chilling tolerance in seedlings of rice (Oryza sativa).-Bot. Bull. Acad. sin 30: 263–273, 1989.Google Scholar
  6. Cohen, P.: The structure and regulation of protein phosphatase.-Annu. Rev. Biochem. 58: 453–508, 1989.PubMedCrossRefGoogle Scholar
  7. Dian, W., Jiang, H., Chen, Q., Liu, F., Wu, P.: Cloning and characterization of the granule-bound starch synthase II gene in rice: gene expression is regulated by the nitrogen level, sugar and circadian rhythm.-Planta 218: 261–268, 2003.PubMedCrossRefGoogle Scholar
  8. Dian, W., Jiang, H., Wu, P.: Evolution and expression analysis of starch synthase III and IV in rice.-J. exp. Bot. 56: 623–632, 2005.PubMedCrossRefGoogle Scholar
  9. Dry, I., Smith, A.M., Edwards, A., Bhattacharyya, M., Dunn, P., Martin, C.: Characterization of cDNAs encoding two isoforms of granule-bound starch synthase which show differential expression in developing storage organs of pea and potato.-Plant J. 2: 193–202, 1992.PubMedGoogle Scholar
  10. Geigenberger, P., Muller-Rober, B., Stitt, M.: Contribution of adenosine 5′-diphosphoglucose pyrophosphorylase to the control of starch synthesis is decreased by water stress in growing potato tubers.-Planta 209: 338–345, 1999a.PubMedCrossRefGoogle Scholar
  11. Geigenberger, P., Reimholz, R., Deiting, U., Sonnewald, U., Stitt, M.: Decreased of expression sucrose phosphate synthase strongly inhibits the water stress-induced synthesis of sucrose in growing potato tubers.-Plant J. 19: 119–129, 1999b.PubMedCrossRefGoogle Scholar
  12. Hirano, H.Y., Sano, Y.: Enhancement of Wx gene expression and the accumulation of amylose in response to cool temperature during seed development in rice.-Plant Cell Physiol. 39: 807–812, 1998.Google Scholar
  13. Hsu, Y.T., Kao, C.H.: Role of abscisic acid in cadmium tolerance of rice (Oryza sativa L.) seedlings.-Plant Cell Environ. 26: 867–874, 2003.PubMedCrossRefGoogle Scholar
  14. Jiang, H.W., Dian, W.M., Wu, P.: Effect of high temperature on fine structure of amylopectin in rice endosperm by reducing the activity of the starch branching enzyme.-Phytochemistry 63: 53–59, 2003.PubMedCrossRefGoogle Scholar
  15. Keeling, P.L., Bacon, P.J., Holt, D.C.: Elevated temperature reduces starch deposition in wheat endosperm by reducing the activity of soluble starch synthase.-Planta 191: 342–348, 1993.CrossRefGoogle Scholar
  16. Manners, D.J.: Starch. In Biochemistry of Storage Carbohydrates in Green Plants.-Academic Press, London 1985.Google Scholar
  17. Marshall, J., Sidebottom, C., Debet, M., Martin, C., Smith, A.M., Edwards, A.: Identification of the major starch synthase in the soluble fraction of potato tubers.-Plant Cell 8: 1121–1135, 1996.PubMedCrossRefGoogle Scholar
  18. Mérida, A., Rodríguez-Galán, J.M., Vincent, C., Romero, J.M.: Expression of the granule-bound starch synthase I (Waxy) gene from snapdragon is developmentally and circadian clock regulated.-Plant Physiol. 120: 401–409, 1999.PubMedCrossRefGoogle Scholar
  19. Pathre, U.V., Sinha, A.K., Shirke, P.A., Ranade, S.A.: Diurnal and seasonal modulation of sucrose phosphate synthase activity in leaves of Prosopis juliflora.-Biol. Plant. 48: 227–235, 2004.CrossRefGoogle Scholar
  20. Seki, M., Narusaka, M., Ishida, J., Nanjo, T., Fujita, M., Oono, Y., Kamiya, A., Nakajima, M., Enju, A., Sakurai, T., Satou, M., Akiyama, K., Taji, T., Yamaguchi-Shinozaki, K., Carninci, P., Kawai, J., Hayashizaki, Y., Shinozaki, K.: Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray.-Plant J. 31: 279–292, 2002.PubMedCrossRefGoogle Scholar
  21. Shinozaki, K., Yamaguchi-Shinozaki, K.: Molecular responses to dehydration and low temperature: differences and crosstalk between two stress signaling pathways.-Curr. Opinion Plant Biol. 3: 217–223, 2000.Google Scholar
  22. Shinozaki, K., Yamaguchi-Shinozaki, K., Seki, M.: Regulatory network of gene expression in the drought and cold stress responses.-Curr. Opinion Plant Biol. 6: 410–417, 2003.CrossRefGoogle Scholar
  23. Sun, W., Van Montagu, M., Verbruggen, N.: Small heat shock proteins and stress tolerance in plants.-Biochim. biophys. Acta 1577: 1–9, 2002.PubMedGoogle Scholar
  24. Suzuki, Y., Sano, Y., Hirano, H.Y.: Isolation and characterization of a rice mutant insensitive to cool temperatures on amylose synthesis.-Euphytica 123: 95–100, 2002.CrossRefGoogle Scholar
  25. Tsai, C.Y.: The function of the Waxy locus in starch synthesis in maize endosperm.-Biochem. Genet. 11: 83–96, 1974.PubMedCrossRefGoogle Scholar
  26. Wang, S.J., Yeh, K.W., Tsai, C.Y.: Molecular characterization and expression of a starch granule-bound starch synthase gene in the sink and source tissues of sweet potato.-Physiol. Plant. 106: 253–261, 1999.CrossRefGoogle Scholar
  27. Wang, S.J., Yeh, K.W., Tsai, C.Y.: Regulation of starch granule-bound starch synthase I gene expression by circadian clock and sucrose in the source tissue of sweet potato.-Plant Sci. 161: 635–644, 2001.CrossRefGoogle Scholar
  28. Wang, S.J., Yeh, K.W., Tsai, C.Y.: Circadian control of sweet potato granule-bound starch synthase I gene in Arabidopsis plants.-Plant Growth Regul. 42: 161–168, 2004.CrossRefGoogle Scholar
  29. Wilhelm, E.P., Mullen, R.E., Keeling, P.L., Singletary, G.W.: Heat stress during grain filling in maize: effects on kernel growth and metabolism.-Crop Sci. 39: 1733–1741, 1999.CrossRefGoogle Scholar
  30. Xiong, L., Schumaker, K.S., Zhu, J.K.: Cell signaling during cold, drought, and salt stress.-Plant Cell 14(Suppl): S165–S183, 2002.PubMedGoogle Scholar

Copyright information

© Institute of Experimental Botany, ASCR, Praha 2006

Authors and Affiliations

  • S. J. Wang
    • 1
  • L. F. Liu
    • 1
  • C. K. Chen
    • 1
  • L. W. Chen
    • 1
  1. 1.Department of AgronomyNational Taiwan UniversityTaipeiTaiwan

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