Biologia Plantarum

, Volume 50, Issue 3, pp 457–460 | Cite as

A modified method of flow cytometric seed screen simplifies the quantification of progeny classes with different ploidy levels

  • A. Krahulcova
  • J. Suda
Brief Communication


Flow cytometric analysis of ten bulked seeds is proposed to quantify particular embryo ploidy classes in Hieracium. The method is recommended 1) for the detection and quantification of residual sexuality in facultative apomicts, which can generate progeny from heteroploid crosses, 2) for the quantitative screening of pollen donors with different ploidy levels, based on the fertilization success of the maternal plant, and 3) for the screening of parents producing a high proportion of polyhaploids.

Additional key words

facultative apomixis polyhaploids reproduction routes seed progeny screening 



flow cytometric seed screen




coefficient of variation


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Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2006

Authors and Affiliations

  1. 1.Institute of BotanyAcademy of Sciences of the Czech RepublicPruhoniceCzech Republic
  2. 2.Department of BotanyCharles UniversityPragueCzech Republic

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