Biologia Plantarum

, Volume 50, Issue 3, pp 457–460 | Cite as

A modified method of flow cytometric seed screen simplifies the quantification of progeny classes with different ploidy levels

Brief Communication


Flow cytometric analysis of ten bulked seeds is proposed to quantify particular embryo ploidy classes in Hieracium. The method is recommended 1) for the detection and quantification of residual sexuality in facultative apomicts, which can generate progeny from heteroploid crosses, 2) for the quantitative screening of pollen donors with different ploidy levels, based on the fertilization success of the maternal plant, and 3) for the screening of parents producing a high proportion of polyhaploids.

Additional key words

facultative apomixis polyhaploids reproduction routes seed progeny screening 



flow cytometric seed screen




coefficient of variation


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Caceres, M.E., Matzk, F., Busti, A., Pupilli, F., Arcioni, S.: Apomixis and sexuality in Paspalum simplex: characterization of the mode of reproduction in segregating progenies by different methods.-Sex. Plant Reprod. 14: 201–206, 2001.Google Scholar
  2. Cronquist, A.: The Compositae revised.-Brittonia 29: 137–153, 1977.Google Scholar
  3. Erdelska, O.: Embryologia Krytosemennych Rastlin. [Embryology of Angiosperms].-Veda, Bratislava 1981.Google Scholar
  4. Harlan, J.R., De Wet, J.M.J.: On O. Winge and a prayer: the origins of polyploidy.-Bot. Rev. 41: 361–390, 1975.Google Scholar
  5. Heenan, P.B., Molloy, B.P.J., Bicknell, R.A., Luo, C.: Levels of apomictic and amphimictic seed formation in a natural population of Coprosma robusta (Rubiaceae) in Riccarton Bush, Christchurch, New Zealand.-New Zeal. J. Bot. 41: 287–291, 2003.Google Scholar
  6. Hegi, G. (ed.): Flora von Mitteleuropa VI/3. Pp. X–XLIV.-Paul Parey Verlag, Berlin-Hamburg 1979.Google Scholar
  7. Koperdakova, J., Brutovska, R., Cellarova, E.: Reproduction pathway analysis of several Hypericum perforatum somaclonal families.-Hereditas 140: 34–41, 2004.CrossRefPubMedGoogle Scholar
  8. Krahulcova, A., Papouskova, S., Krahulec, F.: Reproduction mode in the allopolyploid facultatively apomictic hawkweed Hieracium rubrum (Asteraceae, H. subgen. Pilosella).-Hereditas 141: 19–30, 2004.CrossRefPubMedGoogle Scholar
  9. Matzk, F., Meister, A., Brutovska, R., Schubert, I.: Reconstruction of reproductive diversity in Hypericum perforatum L. opens novel strategies to manage apomixis.-Plant J. 26: 275–282, 2001.CrossRefPubMedGoogle Scholar
  10. Matzk, F., Meister, A., Schubert, I.: An efficient screen for reproductive pathways using mature seeds of monocots and dicots.-Plant J. 21: 97–108, 2000.CrossRefPubMedGoogle Scholar

Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2006

Authors and Affiliations

  1. 1.Institute of BotanyAcademy of Sciences of the Czech RepublicPruhoniceCzech Republic
  2. 2.Department of BotanyCharles UniversityPragueCzech Republic

Personalised recommendations