A modified method of flow cytometric seed screen simplifies the quantification of progeny classes with different ploidy levels
- 161 Downloads
Flow cytometric analysis of ten bulked seeds is proposed to quantify particular embryo ploidy classes in Hieracium. The method is recommended 1) for the detection and quantification of residual sexuality in facultative apomicts, which can generate progeny from heteroploid crosses, 2) for the quantitative screening of pollen donors with different ploidy levels, based on the fertilization success of the maternal plant, and 3) for the screening of parents producing a high proportion of polyhaploids.
Additional key wordsfacultative apomixis polyhaploids reproduction routes seed progeny screening
flow cytometric seed screen
coefficient of variation
Unable to display preview. Download preview PDF.
- Caceres, M.E., Matzk, F., Busti, A., Pupilli, F., Arcioni, S.: Apomixis and sexuality in Paspalum simplex: characterization of the mode of reproduction in segregating progenies by different methods.-Sex. Plant Reprod. 14: 201–206, 2001.Google Scholar
- Cronquist, A.: The Compositae revised.-Brittonia 29: 137–153, 1977.Google Scholar
- Erdelska, O.: Embryologia Krytosemennych Rastlin. [Embryology of Angiosperms].-Veda, Bratislava 1981.Google Scholar
- Harlan, J.R., De Wet, J.M.J.: On O. Winge and a prayer: the origins of polyploidy.-Bot. Rev. 41: 361–390, 1975.Google Scholar
- Heenan, P.B., Molloy, B.P.J., Bicknell, R.A., Luo, C.: Levels of apomictic and amphimictic seed formation in a natural population of Coprosma robusta (Rubiaceae) in Riccarton Bush, Christchurch, New Zealand.-New Zeal. J. Bot. 41: 287–291, 2003.Google Scholar
- Hegi, G. (ed.): Flora von Mitteleuropa VI/3. Pp. X–XLIV.-Paul Parey Verlag, Berlin-Hamburg 1979.Google Scholar