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Biologia Plantarum

, Volume 49, Issue 1, pp 41–45 | Cite as

In vitro culture of Feronia limonia (L.) Swingle from hypocotyl and internodal explants

  • L. V. Hiregoudar
  • H. G. Ashok Kumar
  • H. N. Murthy
Article

Abstract

The hypocotyl and internodal segments from in vitro grown seedlings of Feronia limonia (L.) Swingle (wood apple) were cultivated on Murashige and Skoog’s (1962, MS) medium supplemented with N6-benzyladenine (BA) or adenine (ADE) or kinetin (KN) at 0.5 to 5 µM. The optimum response was recorded on the medium containing 2 µM BA. An average of 12 and 8 shoots were developed from hypocotyl and internodal explants, respectively, after eight weeks of culture. The shoots were excised, and the residual explants were transferred to fresh medium where again they developed shoots. Up to three such passages resulted in the production of shoots from repeatedly subcultured explants and an average of 24 – 36 shoots per explant was obtained. The in vitro developed shoots produced roots when transferred to half strength MS medium supplemented with 1 µM 1-naphthaleneacetic acid (NAA). The developed plantlets were successfully transferred to mixture of soil, sand and coco-peat (1:1:1) and hardened in controlled environment. Hardened plants were transplanted to soil in greenhouse.

Additional key words

adventitious shoots medicinal plant micropropagation organogenesis 

Abbreviations

ADE

adenine

BA

N6-benzyladenine

IAA

indole-3-acetic acid

IBA

indole-3-butyric acid

KN

kinetin

MS

Murashige and Skoog

NAA

1-naphthaleneacetic acid

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Copyright information

© Institute of Experimental Botany 2005

Authors and Affiliations

  • L. V. Hiregoudar
    • 1
  • H. G. Ashok Kumar
    • 1
  • H. N. Murthy
    • 1
  1. 1.Department of BotanyKarnatak UniversityDharwadIndia

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