Rapid and simple method for Al-toxicity analysis in emerging barley roots during germination
- 162 Downloads
The results demonstrate the benefits of using filter-paper-based system for cultivation the germinating barley seeds for Al toxicity or Al tolerance analyses. Due to the high affinity of filter paper to Al monomeric forms, milimolar Al concentrations were required to cause similar Al toxicity symptoms of roots as micromolar Al concentrations in hydroponics: 1 mM Al had no effects on the emerging barley roots, 2 mM Al was moderately toxic but roots showed good recovery, 4 mM Al was highly toxic and 8 mM Al even lethal. Screening of eight barley cultivars revealed different rank of their tolerance to Al. The root growth inhibition positively correlated with the Al concentration in root tips.
Additional key wordsaluminium cell viability cultivation method hematoxylin
Unable to display preview. Download preview PDF.
- Budikova, S., Mistrik, I.: Cultivar characterisation of aluminium tolerance of barley seedlings by root growth, aluminium and callose distribution.-Biologia 54: 447–451, 1999.Google Scholar
- De Lima, M.L., Copeland, L.: The effect of aluminum on the germination of wheat seeds.-J. Plant Nutr. 13: 1489–1497, 1990.Google Scholar
- Kerven, G.L., Edwards, D.G., Asher, C.J., Hallman, P.S., Kokot, S: Aluminium determination in soil solution. II. Short-term colorimetric procedures for the measurement for inorganic monomeric aluminium in the presence of organic acid ligands.-Aust. J. Soil Res. 27: 91–102, 1989.Google Scholar
- Mistrik, I., Tamas, L., Huttova, J., Gejdosova, V.: Accumulation of microsomal polypeptides in barley roots during aluminium stress.-Biol. Plant. 45: 417–421, 2002.Google Scholar
- Tamas, L., Huttova, J., Hajasova, L., Mistrik, I.: The effect of aluminium on polypeptide pattern of cell wall proteins isolated from the roots of Al-sensitive and Al-resistant barley cultivars.-Acta Physiol. Plant. 23: 161–168, 2001.Google Scholar
- Tamas, L., Huttova, J., Mistrik, I.: Inhibition of Al-induced root elongation and enhancement of Al-induced peroxidase activity in Al-sensitive and Al-resistant barley cultivars are positively correlated.-Plant Soil 250: 193–200, 2003.Google Scholar
- Wagatsuma, T.: Effect of nonmetabolic conditions on the uptake of aluminum by plant roots.-Soil Sci. Plant Nutr. 33: 161–175, 1983.Google Scholar
- Zhang, G., Taylor, G.J.: Effects of biological inhibitors on kinetics of aluminium uptake by excised roots and purified cell wall material of aluminium-tolerant and aluminiumsensitive cultivars of Triticum aestivum L.-J. Plant Physiol. 138: 533–539, 1991.Google Scholar