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Influence of lead on membrane permeability and lipoxygenase activity in lupine roots

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Biologia Plantarum

Abstract

Lead nitrate at concentration of 150 mg dm−3 inhibits root growth of Lupinus luteus seedlings by bout 20 %, which is accompanied by an increase of K+ leakage from the root cells. Non-denaturing isoelectric focusing in polyacrylamide slab gel has shown that lead stimulates the activity of most lipoxygenase isoenzymes and induces one additional isoenzyme with pI 6.9.

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Abbreviations

LOX:

lipoxygenase

MDA:

malondialdehyde

TBArm:

thiobarbituric acid reactive metabolites

References

  • Bhattacharejee, S.: Membrane lipid peroxidation, free radical scavengers and ethylene evolution in Amaranthus as affected by lead and cadmium.-Biol. Plant. 40: 131–135, 1998.

    Article  Google Scholar 

  • Bradford, M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.

    PubMed  Google Scholar 

  • De Vos, C.H.R., Schat, H., Vooijs, R., Ernst, W.H.O.: Copper-induced damage to the permeability barrier in roots of Silene cucubalus.-J. Plant Physiol. 135: 164–169, 1989.

    Google Scholar 

  • Gaillard, T., Chan, H.W.S.: Lipoxygenases.-In: Stumpf, P.K. (ed.): The Biochemistry of Plants: a Comprehensive Treatise. Vol. 4 Lipids: Structure and Function. Pp. 131–161. Academic Press, New York 1980.

    Google Scholar 

  • Gonnelli, C., Galardi, F., Gabbrielli R.: Nickel and copper tolerance and toxicity in three Tuscan populations of Silene paradoxa.-Physiol. Plant. 113: 507–514, 2001.

    Article  Google Scholar 

  • Halliwell, B., Gutteridge, J.M.C.: Free Radicals in Biology and Medicine.-Clarendon Press, Oxford 1989.

    Google Scholar 

  • Hartley-Whitaker, J., Ainsworth, G., Meharg, A.A.: Copper-and arsenate-induced oxidative stress in Holcus lanatus L. clones with differential sensitivity.-Plant Cell Environ. 24: 713–722, 2001.

    Article  Google Scholar 

  • Heinisch, O., Kowalski, E., Ludwig, H., Tauscher, B.: Staining for soybean lipoxygenase activity in electrophoretic gels.-Fett/Lipid 5: 193–184, 1996.

    Google Scholar 

  • Hendry, G.A.F., Grime, J.P.: Methods in Comparative Plant Ecology.-Chapman & Hall, London 1993.

    Google Scholar 

  • Jackowski, G., Przymusinski, R.: The resolution and biochemical characterization of subcomplexes of the main light-harvesting chlorophyll a/b-protein complex of photosystem II (LHC II).-Photosynth. Res. 43: 41–48, 1995.

    Article  Google Scholar 

  • Maccarrone, M., Van Aarie, P.G.M., Veldink, G.A., Vliegenthart J.F.G.: In vitro oxygenation of soybean biomembranes by lipoxygenase-2.-Biochim. biophys. Acta 1190: 164–169, 1994.

    PubMed  Google Scholar 

  • Mishra, A., Choudhuri, M.A.: Effects of salicylic acid on heavy metal-induced membrane deterioration mediated by lipoxygenase in rice.-Biol. Plant. 42: 409–415, 1999.

    Article  Google Scholar 

  • Shah, K., Kumar, R.G., Verma, S., Dubey, R.S.: Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings.-Plant Sci. 161: 1135–1144, 2001.

    Article  Google Scholar 

  • Shainberg, O., Rubin, B., Rabinowitch, H.D., Libal, Y., Tel-Or E.: Acclimation of beans to oxidative stress by treatment with sublethal iron levels.-J. Plant Physiol. 157: 93–99, 2000.

    Google Scholar 

  • Siedow, J.N.: Plant lipoksygenase: structure and function.-Annu. Rev. Plant Physiol. Plant mol.Biol. 42: 145–188, 1991.

    Article  Google Scholar 

  • Weckx, J.E.J., Clijsters, H.M.M.: Oxidative damage and defense mechanisms in primary leaves of Phasoeolus vulgaris as a result of root assimilation of toxic amounts of cooper.-Physiol. Plant. 96: 506–512, 1996.

    Article  Google Scholar 

  • Weckx, J.E.J., Clijsters, H.M.M.: Zn phytotoxicity induces oxidative stress in bean leaves Phaseolus vulgaris.-Plant Physiol. Biochem. 35: 405–410, 1997.

    Google Scholar 

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Correspondence to E. A. Gwozdz.

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The work was supported by the State in Committee for Scientific Research (KBN) grant no. 3PO6A 018 23.

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Rucinska, R., Gwozdz, E.A. Influence of lead on membrane permeability and lipoxygenase activity in lupine roots. Biol Plant 49, 617–619 (2005). https://doi.org/10.1007/s10535-005-0059-9

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  • DOI: https://doi.org/10.1007/s10535-005-0059-9

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