Biologia Plantarum

, Volume 49, Issue 3, pp 371–380 | Cite as

Expression of modified 7SL RNA gene in transgenic Solanum tuberosum plants

  • L. Vrba
  • J. Matousek


A modified plant 7SL RNA gene from Arabidopsis thaliana designated AHIIA63M was introduced into potato plants via Agrobacterium-mediated transformation. No transgenic plants could be obtained using pGPTV-based binary vectors where AHIIA63M gene driven by polIII promoter was located close to the polII promoter of the selection gene. Special binary vectors with matrix attachment region (MAR) elements had to be used for transformation to insulate polII and polIII promoters within T-DNA. The level of AHIIA63M RNA in transgenic plants was lower than the levels of transcripts of transgenes driven by RNA polymerase II. The level of AHIIA63M transcript in transgenic potato plants was tissue specific. The highest expression was detected in roots and gynoecium and the lowest in tubers. Moreover, non-specific promoter activity within the MAR element was revealed. This activity contributed to AHIIA63M transcription. This is the first report of expression of a modified 7SL RNA gene in transgenic plants and promoter activity within the MAR element.

Additional key words

RNA polymerase III polIII expression cassette MAR element 


A element

chicken lysozyme A element




matrix attachment region


neomycin phosphotransferase II


cauliflower mosaic virus 35S promoter


nopaline synthase promoter


RNA polymerase II


RNA polymerase III


signal recognition particle


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Copyright information

© Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 2005

Authors and Affiliations

  1. 1.Department of Molecular Genetics, Institute of Plant Molecular BiologyAcademy of Sciences of the Czech RepublicCeske BudejoviceCzech Republic

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