Citrate analysis using capillary electrophoresis and complexation with Eu3+-tetracycline
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A sensitive assay for citrate was developed. Citrate was incubated with 50 μM Eu3+-tetracycline and the complex separated using capillary electrophoresis utilizing post-column laser-induced luminescence detection in a sheath flow cuvette. Signal was linear with citrate concentration from 10 μM to 200 nM. Injection volumes were 320 pL. For the 200 nM sample, this corresponds to the injection of 64 amoles of citrate. Separation time was < 90 s with a total run time of 5 min. As an application the method was used to analyze citrate in agricultural and medicinal products. The method was also used to develop an assay for the enzyme citrate synthase.
KeywordsCapillary electrophoresis Citrate Citrate synthase Eu3+-tetracycline Luminescence
This study was supported by a Grant from the Natural Sciences and Engineering Research Council.
- Apleblat A (2014) Citric Acid, Springer, ISBN 978-3-319-11232-9Google Scholar
- Kaleemullah T, Ahmed M, Sharma HK, Rao MN (2011) Sensitive ion chromatographic determination of citrate and formate in pharmaceuticals. Rasayan J Chem 4:844–852Google Scholar
- Luo LY, Luo L, Luo YH, Wang CY, Jiang C, Chen Q, Luo M, Long F, Wang S, Tan MG, Chen D (2013) Determination of potassium citrate and sodium citrate chewable tablets content and related substances by RP-HPLC. Lat Am J Pharm 32:37–42Google Scholar
- Shriver D, Weller M, Overton T, Rourke J, Armstrong F (2014) Inorganic chemistry, 6th edn. WH Freeman and Company, New YorkGoogle Scholar
- Voet D, Voet JG, Pratt CW (2016) Fundamentals of biochemistry: life at the molecular level, 5th edn. Wiley, HobokenGoogle Scholar
- Yamaguchi Y, Hashino K, Ito M, Ikawa K, Nishioka T, Matsumoto K (2009) Sodium dodecyl sulfate polyacrylamide slab gel electrophoresis and hydroxyethyl cellulose gel capillary electrophoresis of luminescent lanthanide chelate-labeled proteins with time-resolved detection. Anal Sci 25:327–332CrossRefGoogle Scholar