BioMetals

, Volume 31, Issue 3, pp 381–398 | Cite as

Are lactoferrin receptors in Gram-negative bacteria viable vaccine targets?

  • Clement Chan
  • Vahid F. Andisi
  • Dixon Ng
  • Nick Ostan
  • Warren K. Yunker
  • Anthony B. Schryvers
Article

Abstract

A number of important Gram-negative pathogens that reside exclusively in the upper respiratory or genitourinary tract of their mammalian host rely on surface receptors that specifically bind host transferrin and lactoferrin as a source of iron for growth. The transferrin receptors have been targeted for vaccine development due to their critical role in acquiring iron during invasive infection and for survival on the mucosal surface. In this study, we focus on the lactoferrin receptors, determining their prevalence in pathogenic bacteria and comparing their prevalence in commensal Neisseria to other surface antigens targeted for vaccines; addressing the issue of a reservoir for vaccine escape and impact of vaccination on the microbiome. Since the selective release of the surface lipoprotein lactoferrin binding protein B by the NalP protease in Neisseria meningitidis argues against its utility as a vaccine target, we evaluated the release of outer membrane vesicles, and transferrin and lactoferrin binding in N. meningitidis and Moraxella catarrhalis. The results indicate that the presence of NalP reduces the binding of transferrin and lactoferrin by cells and native outer membrane vesicles, suggesting that NalP may impact all lipoprotein targets, thus this should not exclude lactoferrin binding protein B as a target.

Keywords

Lactoferrin-binding protein Transferrin binding protein Vaccine Antimicrobial peptides 

Notes

Acknowledgements

This work was supported by funding from the Canadian Institutes of Health Research (Grant MOP138273), and the National Sciences and Engineering Council (RGPIN-2016-04555) for ABS.

Funding

This study was supported by the Canadian Institutes of Health Research (Grant Number MOP138273) and the National Sciences and Engineering Council of Canada (Discovery Grant 298351-2010).

Compliance with ethical standards

Conflict of interest

ABS is a stakeholder in Engineered Antigens Inc.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Clement Chan
    • 1
  • Vahid F. Andisi
    • 1
  • Dixon Ng
    • 1
  • Nick Ostan
    • 2
  • Warren K. Yunker
    • 3
  • Anthony B. Schryvers
    • 1
  1. 1.Department of Microbiology, Immunology & Infectious DiseasesUniversity of CalgaryCalgaryCanada
  2. 2.Department of BiochemistryUniversity of TorontoTorontoCanada
  3. 3.Department of SurgeryUniversity of CalgaryCalgaryCanada

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