BioMetals

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Six indicator genes for zinc (Zn) homeostasis in freshwater teleost yellow catfish Pelteobagrus fulvidraco: molecular characterization, mRNA tissue expression and transcriptional changes to Zn exposure

  • Guang-Hui Chen
  • Zhi Luo
  • Chuan-Chuan Wei
  • Dan-Dan Li
  • Ya-Xiong Pan
Article
  • 27 Downloads

Abstract

Excessive Zn in the aquatic environment can be toxic and causes dysfunction in Zn homeostasis for fish, which ultimately influences the function of various biological processes. Zn homeostasis is controlled by Zn transporters. This study cloned and characterized the full-length cDNA sequences of six Zn transport-relevant genes (ZnT1, ZnT5, ZnT7, ZIP4, ZIP5 and MTF-1) from yellow catfish Pelteobagrus fulvidraco. The six genes share similar domains to their corresponding members of mammals. Their mRNA amounts were widely existent across eight tissues (intestine, liver, brain, heart, gill, muscle, spleen and mesenteric fat), but relatively predominant in the liver and intestine. On day 28, Zn exposure tended to increase transcript levels of ZnT1, ZnT5 and MTF-1, decrease hepatic ZIP5 expression, but did not significantly affect the expression of ZnT7 and ZIP4. On day 56, Zn exposure tended to increase transcript levels of ZnT1 and MTF-1, down-regulate hepatic mRNA amounts of ZIP4 and ZIP5; among three Zn treatments, ZnT5 expression in the 0.5 mg Zn/L group and ZnT7 expression in the 0.25 mg Zn/L group were the highest. The mRNA abundances of these genes showed Zn concentration- and exposure time-dependent manners. For the first time, we characterized the full-length cDNA sequences of six Zn transport-relevant genes in fish, explored their tissue expression profiles and transcriptional responses to Zn exposure. Our study built good basis for further investigating their physiological functions of these genes and provided new insights into the regulatory mechanisms of Zn homeostasis in fish.

Keywords

Pelteobagrus fulvidraco Zn exposure Zn transporter Zn homeostasis 

Abbreviations

Aa

Amino acid

ANOVA

One-way analysis of variance

Asp

Aspartic acid

His

Histidine

MTF-1

Metal response element binding transcription factor-1

NES

Nuclear exclusion sequence

NLS

Nuclear localization sequence

ORF

Open reading frame

Pf

Pelteobagrus fulvidraco

qPCR

Real-time fluorescence quantitative PCR

SEM

Standard error of means

SP

Signal peptide

TMD

Transmembrane domain

Zn

Zinc

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant no. 31422056).

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest with the contents of this article.

Supplementary material

10534_2018_99_MOESM1_ESM.doc (733 kb)
Supplementary material 1 (DOC 733 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery CollegeHuazhong Agricultural UniversityWuhanChina

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