Advertisement

BioMetals

, Volume 28, Issue 4, pp 701–712 | Cite as

Metallothionein, essential elements and lipid peroxidation in mercury-exposed suckling rats pretreated with selenium

  • Tatjana Orct
  • Maja Lazarus
  • Marija Ljubojević
  • Ankica Sekovanić
  • Ivan Sabolić
  • Maja Blanuša
Article

Abstract

Detoxification of mercury (Hg) with selenium (Se) in the early postnatal period with regard to the expression of metallothionein protein (MT), essential element status, and lipid peroxidation level in tissues has not been studied. Seven-day-old Wistar pups were orally pretreated with Se [6 μmol Na2SeO3/kg body weight (b.w.)] for 3 days and then cotreated with Hg (6 μmol HgCl2/kg b.w.) for the following 4 days. This group (Se + Hg) was compared to the groups treated with Hg, Se, or vehicle (control). Compared to the Hg-group, Se + Hg-group exhibited lower renal MT expression, reduced accumulation of Hg, Cu and Zn, and reduced excretion of Se, Hg and Zn in urine. In the liver, MT was stimulated by Se treatment in both, Se and Se + Hg-group. Hepatic and brain levels of the endogenous essential elements Cu, Fe, Mg, and Zn remained unchanged in all of the studied groups. Brain Hg levels and oxidation of lipids measured as thiobarbituric acid reactive substances were diminished in Se + Hg-group of pups compared to the Hg-group. This study suggests that Se pretreatment can help reduce Hg in the tissues of suckling rats, simultaneously preventing impairment of essential element levels in the kidneys and their excessive excretion via urine. Also, Se was shown to prevent oxidative damage of lipids in the brain, which is particularly susceptible to Hg during the early postnatal period.

Keywords

Selenium supplementation Mercury exposure Suckling rat Metallothionein Essential element Lipid peroxidation 

Notes

Acknowledgments

This work was supported by the Ministry of Science Education and Sports of the Republic of Croatia (Project Grants No. 022-0222148-2135 and 022-0222148-2146). The technical assistance of Mrs. Đurđa Breški, Marija Ciganović and Snježana Mataušić is gratefully acknowledged. The authors thank Mr. Makso Herman for language advice.

References

  1. Abdulla M, Chmielnicka J (1990) New aspects on the distribution and metabolism of essential trace elements after dietary exposure to toxic metals. Biol Trace Elem Res 23:25–53CrossRefGoogle Scholar
  2. Agarwal R, Behari JR (2007) Effect of selenium pretreatment in chronic mercury intoxication in rats. Bull Environ Contam Toxicol 79:306–310. doi: 10.1007/s00128-007-9226-3 PubMedCrossRefGoogle Scholar
  3. Agarwal R, Raisuddin S, Tewari S, Goel SK, Raizada RB, Behari JR (2010) Evaluation of comparative effect of pre- and posttreatment of selenium on mercury-induced oxidative stress, histological alterations, and metallothionein mRNA expression in rats. J Biochem Mol Toxicol 24:123–135. doi: 10.1002/jbt.20320 PubMedGoogle Scholar
  4. Agha FE, Youness ER, Selim MMH, Ahmed HH (2014) Nephroprotective potential of selenium and taurine against mercuric chloride induced nephropathy in rats. Ren Fail 36:704–716. doi: 10.3109/0886022X.2014.890012 PubMedCrossRefGoogle Scholar
  5. ATSDR (1999) Toxicological profile for mercury. U.S. Department of Health and Human Services, Public Health Service, Agency for Toxic Substances and Disease Registry, Atlanta. http://www.atsdr.cdc.gov/ToxProfiles/tp46.pdf. Accessed 15 Nov 2014
  6. Bogden JD, Kemp FW, Troiano RA, Jortner BS, Timpone C, Giuliani D (1980) Effect of mercuric chloride and methylmercury chloride exposure on tissue concentrations of six essential minerals. Environ Res 21:350–359PubMedCrossRefGoogle Scholar
  7. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254PubMedCrossRefGoogle Scholar
  8. Brambila E, Liu J, Morgan DL, Beliles RP, Waalkes MP (2002) Effect of mercury vapor exposure on metallothionein and glutathione S-transferase gene expression in the kidney of nonpregnant, pregnant, and neonatal rats. J Toxicol Environ Health A 65:1273–1288. doi: 10.1080/00984100290071405 PubMedCrossRefGoogle Scholar
  9. Brandão R, Borges LP, Nogueira CW (2009) Concomitant administration of sodium 2,3-dimercapto-1-propanesulphonate (DMPS) and diphenyl diselenide reduces effectiveness of DMPS in restoring damage induced by mercuric chloride in mice. Food Chem Toxicol 47:1771–1778. doi: 10.1016/j.fct.2009.04.035 PubMedCrossRefGoogle Scholar
  10. Bridges CC, Zalups RK (2010) Transport of inorganic mercury and methylmercury in target tissues and organs. J Toxicol Environ Health B 13:385–410. doi: 10.1080/10937401003673750 CrossRefGoogle Scholar
  11. Cherian MG, Templeton DM, Gallant KR, Banerjee D (1987) Biosynthesis and metabolism of metallothionein in rat during perinatal development. Exp Suppl 52:499–505CrossRefGoogle Scholar
  12. Chmielnicka J, Brzeźnicka E, Sniady A (1986) Kidney concentrations and urinary excretion of mercury, zinc and copper following the administration of mercuric chloride and sodium selenite to rats. Arch Toxicol 59:16–20PubMedCrossRefGoogle Scholar
  13. Chowdhury BA, Chandra RK (1987) Biological and health implications of toxic heavy metal and essential trace element interactions. Prog Food Nutr Sci 11:55–113PubMedGoogle Scholar
  14. Clarkson TW (1997) The toxicology of mercury. Crit Rev Clin Lab Sci 34:369–403PubMedCrossRefGoogle Scholar
  15. Clarkson TW, Magos L (2006) The toxicology of mercury and its chemical compounds. Crit Rev Toxicol 36:609–662PubMedCrossRefGoogle Scholar
  16. Ercal N, Gure-Orhan H, Aykin-Burns N (2001) Toxic metals and oxidative stress Part I: mechanisms involved in metal induced oxidative damage. Curr Top Med Chem 1:529–539PubMedCrossRefGoogle Scholar
  17. Falnoga I, Tušek-Žnidarič M (2007) Selenium–mercury interactions in man and animals. Biol Trace Elem Res 119:212–220. doi: 10.1007/s12011-007-8009-3 PubMedCrossRefGoogle Scholar
  18. Farina M, Brandão R, de Lara FS, Pagliosa LB, Soares FA, Souza DO, Rocha JB (2003) Profile of nonprotein thiols, lipid peroxidation and delta-aminolevulinate dehydratase activity in mouse kidney and liver in response to acute exposure to mercuric chloride and sodium selenite. Toxicology 184:179–187PubMedCrossRefGoogle Scholar
  19. Feng W, Wang M, Li B, Liu J, Chai Z, Zhao J, Deng G (2004) Mercury and trace element distribution in organic tissues and regional brain of fetal rat after in utero and weaning exposure to low dose of inorganic mercury. Toxicol Lett 152:223–234. doi: 10.1016/j.toxlet.2004.05.001 PubMedCrossRefGoogle Scholar
  20. Fernandez EL, Dencker L, Tallkvist J (2007) Expression of ZnT-1 (Slc30a1) and MT-1 (Mt1) in the conceptus of cadmium treated mice. Reprod Toxicol 24:353–358. doi: 10.1016/j.reprotox.2007.06.006 PubMedCrossRefGoogle Scholar
  21. Grandjean P et al (2008) The Faroes statement: human health effects of developmental exposure to chemicals in our environment. Basic Clin Pharmacol Toxicol 102:73–75. doi: 10.1111/j.1742-7843.2007.00114.x PubMedGoogle Scholar
  22. Haase H, Maret W (2008) Partial oxidation and oxidative polymerization of metallothionein. Electrophoresis 29:4165–4176CrossRefGoogle Scholar
  23. Iwai N, Watanabe C, Suzuki T, Suzuki KT, Tohyama C (1988) Metallothionein induction by sodium selenite at two different ambient temperatures in mice. Arch Toxicol 62:447–451PubMedCrossRefGoogle Scholar
  24. Khan MA, Wang F (2009) Mercury–selenium compounds and their toxicological significance: toward a molecular understanding of the mercury–selenium antagonism. Environ Toxicol Chem 28:1567–1577. doi: 10.1897/08-375.1 PubMedCrossRefGoogle Scholar
  25. Kostial K, Šimonović I, Pišonić M (1971) Lead absorption from the intestine in newborn rats. Nature 233:564PubMedCrossRefGoogle Scholar
  26. Kostial K, Kello D, Jugo S, Rabar I, Maljković T (1978) Influence of age on metal metabolism and toxicity. Environ Health Perspect 25:81–86PubMedCentralPubMedCrossRefGoogle Scholar
  27. Liu X, Jin T, Nordberg GF (1991) Increased urinary calcium and magnesium excretion in rats injected with mercuric chloride. Pharmacol Toxicol 68:254–259PubMedCrossRefGoogle Scholar
  28. Liu X, Nordberg GF, Jin T (1992) Increased urinary excretion of zinc and copper by mercuric chloride injection in rats. Biometals 5:17–22PubMedCrossRefGoogle Scholar
  29. Luque-Garcia JL, Cabezas-Sanchez P, Anunciação DS, Camara C (2013) Analytical and bioanalytical approaches to unravel the selenium–mercury antagonism: a review. Anal Chim Acta 801:1–13. doi: 10.1016/j.aca.2013.08.043 PubMedCrossRefGoogle Scholar
  30. Magos L, Webb M (1976) Differences in distribution and excretion of selenium and cadmium or mercury after their simultaneous administration subcutaneously in equimolar doses. Arch Toxicol 36:63–69PubMedCrossRefGoogle Scholar
  31. Maret W (2000) The function of zinc metallothionein: a link between cellular zinc and redox state. J Nutr 130(5S Suppl):1455S–1458SPubMedGoogle Scholar
  32. Mehra RK, Bremner I (1984) Metallothionein-I in the plasma and liver of neonatal rats. Biochem J 217:859–862PubMedCentralPubMedGoogle Scholar
  33. Miller RK (1983) Perinatal toxicology: its recognition and fundamentals. Am J Ind Med 4:205–244PubMedCrossRefGoogle Scholar
  34. Mizzen CA, Cartel NJ, Yu WH, Fraser PE, McLachlan DR (1996) Sensitive detection of metallothioneins-1, -2 and -3 in tissue homogenates by immunoblotting: a method for enhanced membrane transfer and retention. J Biochem Biophys Methods 32:77–83PubMedCrossRefGoogle Scholar
  35. Nielsen JB, Andersen O (1991) A comparison of the effects of sodium selenite and seleno-l-methionine on disposition of orally administered mercuric chloride. J Trace Elem Electrolytes Health Dis 5:245–250PubMedGoogle Scholar
  36. Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358PubMedCrossRefGoogle Scholar
  37. Orct T, Lazarus M, Jurasović J, Blanuša M, Piasek M, Kostial K (2009) Influence of selenium dose on mercury distribution and retention in suckling rats. J Appl Toxicol 29:585–589. doi: 10.1002/jat.1444 PubMedCrossRefGoogle Scholar
  38. Peixoto NC, Serafim MA, Flores EMM, Bebianno MJ, Pereira ME (2007) Metallothionein, zinc, and mercury levels in tissues of young rats exposed to zinc and subsequently to mercury. Life Sci 81:1264–1271. doi: 10.1016/j.lfs.2007.08.038 PubMedCrossRefGoogle Scholar
  39. Peixoto NC, Rocha LC, Moraes DP, Bebianno MJ, Dressler VL, Flores EM, Pereira ME (2008) Changes in levels of essential elements in suckling rats exposed to zinc and mercury. Chemosphere 72:1327–1332. doi: 10.1016/j.chemosphere.2008.04.027 PubMedCrossRefGoogle Scholar
  40. Peraza MA, Ayala-Fierro F, Barber DS, Casarez E, Rael LT (1998) Effects of micronutrients on metal toxicity. Environ Health Perspect 106(Suppl 1):203–216PubMedCentralPubMedCrossRefGoogle Scholar
  41. Perottoni J, Lobato LP, Silveira A, Rocha JB, Emanuelli T (2004a) Effects of mercury and selenite on delta-aminolevulinate dehydratase activity and on selected oxidative stress parameters in rats. Environ Res 95:166–173. doi: 10.1016/j.envres.2003.08.007 PubMedCrossRefGoogle Scholar
  42. Perottoni J, Rodrigues OE, Paixão MW, Zeni G, Lobato LP, Braga AL, Rocha JB, Emanuelli T (2004b) Renal and hepatic ALA-D activity and selected oxidative stress parameters of rats exposed to inorganic mercury and organoselenium compounds. Food Chem Toxicol 42:17–28. doi: 10.1016/j.fct.2003.08.002 PubMedCrossRefGoogle Scholar
  43. Ralston NV, Raymond LJ (2010) Dietary selenium’s protective effects against methylmercury toxicity. Toxicology 278:112–123. doi: 10.1016/j.tox.2010.06.004 PubMedCrossRefGoogle Scholar
  44. Romero A, Ramos E, de Los Ríos C, Egea J, Del Pino J, Reiter RJ (2014) A review of metal-catalyzed molecular damage: protection by melatonin. J Pineal Res 56:343–370. doi: 10.1111/jpi.12132 PubMedCrossRefGoogle Scholar
  45. Rooney JP (2007) The role of thiols, dithiols, nutritional factors and interacting ligands in the toxicology of mercury. Toxicology 234:145–156. doi: 10.1016/j.tox.2007.02.016 PubMedCrossRefGoogle Scholar
  46. Sabolić I, Breljak D, Škarica M, Herak-Kramberger CM (2010) Role of metallothionein in cadmium traffic and toxicity in kidneys and other mammalian organs. Biometals 23:897–926. doi: 10.1007/s10534-010-9351-z PubMedCrossRefGoogle Scholar
  47. Su L, Wang M, Yin ST, Wang HL, Chen L, Sun LG, Ruan DY (2008) The interaction of selenium and mercury in the accumulations and oxidative stress of rat tissues. Ecotoxicol Environ Saf 70:483–489. doi: 10.1016/j.ecoenv.2007.05.018 PubMedCrossRefGoogle Scholar
  48. Telišman S (1995) Interactions of essential and/or toxic metals and metalloid regarding interindividual differences in susceptibility to various toxicants and chronic diseases in man. Arh Hig Rada Toksikol 46:459–476PubMedGoogle Scholar
  49. U.S. Environmental Protection Agency, EPA (2002) Child-specific exposure factors handbook. EPA/600/P-00/002B. National Center for Environmental Assessment, Washington, DC. http://www.epa.gov/ncea. Accessed 10 Dec 2014
  50. U.S. Environmental Protection Agency, EPA (2007) Inorganic mercury. Toxicity and exposure assessment for children’s health (TEACH) chemical summary. http://www.epa.gov/teach/chem_summ/mercury_inorg_summary.pdf. Accessed 15 Nov 2014
  51. Watanabe C (2002) Modification of mercury toxicity by selenium: practical importance? Tohoku J Exp Med 196:71–77PubMedCrossRefGoogle Scholar
  52. WHO (1986) Environmental Health Criteria 59: principles for evaluating health risks from chemicals during infancy and childhood: the need for a special approach. World Health Organization, GenevaGoogle Scholar
  53. Yang D-Y, Chen Y-W, Gunn JM, Belzile N (2008) Selenium and mercury in organisms: interactions and mechanisms. Environ Rev 16:71–92. doi: 10.1139/A08-001 CrossRefGoogle Scholar
  54. Zalups RK (2000) Molecular interactions with mercury in the kidney. Pharmacol Rev 52:113–144PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Tatjana Orct
    • 1
  • Maja Lazarus
    • 1
  • Marija Ljubojević
    • 2
  • Ankica Sekovanić
    • 1
  • Ivan Sabolić
    • 2
  • Maja Blanuša
    • 1
  1. 1.Analytical Toxicology and Mineral Metabolism UnitInstitute for Medical Research and Occupational HealthZagrebCroatia
  2. 2.Molecular Toxicology UnitInstitute for Medical Research and Occupational HealthZagrebCroatia

Personalised recommendations