Effects of lanthanum chloride on glutamate level, intracellular calcium concentration and caspases expression in the rat hippocampus
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Lanthanum chloride (LaCl3) can affect neurobehavioral development and impair cognitive abilities. The mechanism underlying LaCl3-induced neurotoxic effects is still unknown. The purpose of this research was to investigate the neuronal impairment induced by LaCl3 and discuss the possible mechanism from the aspects of the alteration of glutamate level, intracellular calcium concentration ([Ca2+]i), Bax, Bcl-2 and caspases expression in the hippocampus. Lactational rats were exposed to 0, 0.25, 0.50 and 1.0 % LaCl3 in drinking water, respectively. Their offspring were exposed to LaCl3 by parental lactation and then administrated with 0, 0.25, 0.50 and 1.0 % LaCl3 in drinking water for 1 month. The results showed that 0.25, 0.50 and 1.0 % LaCl3 exposure induced neuronal impairment in the hippocampus of young rat. Hippocampal glutamate level, [Ca2+]i and ratio of Bax and Bcl-2 expression increased significantly after LaCl3 exposure. Besides, LaCl3 exposure increased GRP78, GRP94, GADD153 and p-JNK expression, promoted the activation of caspase-3, caspase-9 and caspase-12, induced PARP cleavage and caused excessive apoptosis. These results indicate that LaCl3 increases glutamate level, [Ca2+]i and ratio of Bax and Bcl-2 expression, which cause excessive apoptosis by the mitochondrial and endoplasmic reticulum stress-induced pathway, and thus neuronal damages in the hippocampus.
KeywordsLanthanum Glutamate Intracellular calcium Caspases Hippocampus
This work was supported by National Natural Science Foundation of China (No. 30800925 and 81072316).
Conflict of interest
The authors declare that there are no conflicts of interest.
- Abcouwer SF, Marjon PL, Loper RK, Vander Jagt DL (2002) Response of VEGF expression to amino acid deprivation and inducers of endoplasmic reticulum stress. Investig Ophthalmol Vis Sci 43:2791–2798Google Scholar
- Chen ZY (2005) Accumulation and toxicity of rare earth elements in brain and their potential effects on health. Rural Eco-Environ 21(72–73):80 (in Chinese)Google Scholar
- Fan GQ, Yuan ZK, Zheng HL, Liu ZJ (2004) Study on the effects of exposure to rare earth elements and health-responses in children aged 7–10 years. Hyg Res 33:23–28Google Scholar
- Groenendyk J, Michalak M (2005) Endoplasmic reticulum quality control and apoptosis. Acta Biochim Polonica 52:381–395Google Scholar
- Liapi C, Zarros A, Theocharis S, Al-Humadi H, Anifantaki F, Gkrouzman E, Mellios Z, Skandal N, Tsakiris S (2009) The neuroprotective role of l-cysteine towards the effects of short-term exposure to lanthanum on the adult rat brain antioxidant status and the activities of acetylcholinesterase, (Na+, K+)- and Mg2+-ATPase. Biometals 22:329–335PubMedCrossRefGoogle Scholar
- Liu HX, Yang XD, Wang K (2006) Effects of Lanthanide ions (La3+, Gd3+ and Yb3+) on growth of human normal liver line 7701 and human cervical carcinoma and the effect of cell apoptosis induced by lanthanide. J Chin Rare Earth Soc 24:484–488Google Scholar
- Zhu WF, Xu SQ, Zhang H, Shao PP, Wu DS, Yang WJ, Feng J (1996) Investigation of children intelligence quotient in REE mining area: bio-effect study of REE mining area in South Jiangxi. Chin Sci Bull 41:914–916 (in Chinese)Google Scholar