Genome-wide transcriptome analysis of the adaptive response of Enterococcus faecalis to copper exposure
- 356 Downloads
In this work we investigated the adaptive response of E. faecalis to Cu and the role of CopY, a Cu-dependent repressor, in the regulation of Cu metabolism. In doing so, we examined the whole-genome transcriptional response of E. faecalis wild-type (WT) and a ΔcopY strain exposed to non-toxic Cu excess. The results indicated that after Cu exposure, most of the genes that displayed a significant change in their expression levels in the WT strain (135 of the 145 up-regulated genes and 115 of the 142 down-regulated genes) were also differentially expressed in the E. faecalis ΔcopY strain. This extensive overlap in the transcriptional response, suggested that additional transcription factors mediate the response of E. faecalis to Cu. As a first step to analyze this possibility, we selected among the up-regulated genes five genes encoding putative transcriptional regulators and determined their expression levels at different times after Cu exposure. The temporal expression of these regulators was different from that of copY, which reached its maximum at the earliest time measured. Nevertheless, transcription elongation factor GreA, and members of Rrf2, Cro/CI and SorC/DeoR transcription factor families were induced shortly after Cu exposure, suggesting that these proteins are able to complement the role of CopY in the regulatory network activated by Cu. To our knowledge, this is the first report on the global transcriptional response to Cu in a member of this taxonomic group.
KeywordsE. faecalis Copper homeostasis Global gene expression Real-time RT-PCR CopY
This work was supported by grants Basal-CMM, Fondecyt 1071083 and 1090211 and Fondef D04I1257. AR-J was recipients of an ‘A. Steckel’ fellowship from INTA and MAL is a recipient of doctoral fellowship from Conicyt. The authors thank Arely González and Andres Aravena for COGs assignments and manual revision of V583 genome.
- del Pozo T, Cambiazo V, González M (2010) Gene expression profiling analysis of copper homeostasis in Arabidopsis thaliana. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2010.01.111
- Ruiz-Garbajosa P, Bonten MJ, Robinson DA, Top J, Nallapareddy SR, Torres C, Coque TM, Cantón R, Baquero F, Murray BE, del Campo R, Willems RJ (2006) Multilocus sequence typing scheme for Enterococcus faecalis reveals hospital-adapted genetic complexes in a background of high rates of recombination. J Clin Microbiol 44:2220–2228CrossRefPubMedGoogle Scholar