, 22:941 | Cite as

Influences of magnesium deficiency and cerium on antioxidant system of spinach chloroplasts

  • Yuguan Ze
  • Sitao Yin
  • Zhe Ji
  • Luyang Luo
  • Chao Liu
  • Fashui Hong


Magnesium-deficiency conditions applied to spinach cultures caused an oxidative stress status in spinach chloroplast monitored by an increase in reactive oxygen species (ROS) accumulation. The enhancement of lipids peroxide of spinach chloroplast grown in magnesium-deficiency media suggested an oxidative attack that was activated by a reduction of antioxidative defense mechanism measured by analysing the activities of superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase, and glutathione reductase, as well as antioxidants such as carotenoids and glutathione content. As the antioxidative response of chloroplast was reduced in spinach grown in magnesium-deficiency media, it caused a significant reduction of spinach plant weight, old leaves turning chlorosis. However, cerium treatment grown in magnesium-deficiency conditions decreased the malondialdehyde and ROS, and increased activities of the antioxidative defense system, and improved spinach growth. Together, the experimental study implied that cerium could partly substitute for magnesium and increase the oxidative stress-resistance of spinach chloroplast grown in magnesium-deficiency conditions, but the mechanisms need further study.


Cerium Magnesium-deficiency Spinach Chloroplast Antioxidant system 



This work was supported by the National Natural Science Foundation of China (grant No. 30800068).


  1. Able AJ, Guest DI, Sutherland MW (1998) Use of a new tetrazolium-based assay to study the prodution of superoxide radicals by tobacco cell cultures challenged with avirulent zoopspores of phytophthora parasitca var nicotianae. Plant Physiol 117:491–499. doi: 10.1104/pp.117.2.491 CrossRefPubMedGoogle Scholar
  2. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenol oxidase in beta vulgaris. Plant Physiol 24:1–15. doi: 10.1104/pp.24.1.1 CrossRefPubMedGoogle Scholar
  3. Cakma KI (1994) Activity of ascorbate-dependent H2O2-scavenging enzymes and leaf chlorosis are enchanced in magnesium and potassium deficient leaves, but not in phosphorus-deficient leaves. J Exp Bot 45(278):1259–1266. doi: 10.1093/jxb/45.9.1259 CrossRefGoogle Scholar
  4. Cakma KI, Hen Gel ERC, Marschn ERH (1994) Changes in phloem export of sucrose in leaves in response to phosphorous, potassium and magnesium deficiency in bean plant. J Exp Bot 45:1251–1257. doi: 10.1093/jxb/45.9.1251 CrossRefGoogle Scholar
  5. Candan N, Tarhan L (2003) Relationship among chlorophyll-carotenoid content, antioxidant enzyme activities and lipid peroxidation levels by Mg2+ deficiency in the Mentha leaves. Plant Physiol Biochim 41:35–40CrossRefGoogle Scholar
  6. Chris B, Robert F (2000) The role of calcium and activated oxygens as signals for controlling cross-tolerance. Trends Plant 5(6):241–246. doi: 10.1016/S1360-1385(00)01628-9 CrossRefGoogle Scholar
  7. Ginnopolitis CN, Rice SK (1977) Superoxide dismutase purification and quantitative relationship with water soluble protein in seedling. Plant Physiol 59:315–318. doi: 10.1104/pp.59.2.315 CrossRefGoogle Scholar
  8. Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198. doi: 10.1016/0003-9861(68)90654-1 CrossRefPubMedGoogle Scholar
  9. Hissin PJ, Hilf R (1976) A fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal Biochem 74:214–226. doi: 10.1016/0003-2697(76)90326-2 CrossRefPubMedGoogle Scholar
  10. Hong FS, Wei ZG, Zhao GW (2000) Effect of lanthanum on aged seed germination of rice. Biol Trace Elem Res 75:205–213. doi: 10.1385/BTER:75:1-3:205 CrossRefGoogle Scholar
  11. Hong FS, Wang L, Meng XX, Wei ZG, Zhao GW (2002a) The effect of cerium on the chlorophyll formation of spinach. Biol Trace Elem Res 89:263–277. doi: 10.1385/BTER:89:3:263 CrossRefGoogle Scholar
  12. Hong FS, Wei ZG, Zhao GW (2002b) Mechanism of lanthanum effect on the chlorophyll of spinach. Sci China Ser C 45(2):166–176. doi: 10.1360/02yc9019 CrossRefGoogle Scholar
  13. Hong FS, Wang XF, Liu C, Su GX, Song WP, Wu K, Tao Y, Zhao GW (2003) Effect of Ce3+ on spectral characteristic of D1/D2/Cytb559 complex from spinach. Sci China Ser B 46(1):42–50Google Scholar
  14. Hong FS, Wang L, Tao Y (2005) Mechanism of LaCl3 on increasing photosystem II activity of spinach. Chin J Chem 23:617–621. doi: 10.1002/cjoc.200590617 CrossRefGoogle Scholar
  15. Huang H, Liu XQ, Qu CX, Liu C, Chen L, Hong FS (2008) Influences of calcium deficiency and cerium on the conversion efficiency of light energy of spinach. Biometals 21:553–561. doi: 10.1007/s10534-008-9141-z CrossRefPubMedGoogle Scholar
  16. John G, Scandalios JG (1993) Oxygen stress and superoxide dismutase. Plant Physiol 101:7–12Google Scholar
  17. Li Y, Zhang JL, Chen QL, Zhang WM (1998) The effects of magnesium deficiency on some physiological indexes in relation to carbon and nitrogen metobolism in longan (Dimocarpus Longana Lour). J Fujian Agric Univ 13(Suppl):49–53 in chinGoogle Scholar
  18. Li Y, Liu XH, Zhang WM (2000) Advances in magnesium nutritional physiology in plants. J Fujian Agric Univ 29(1):74–80 in chinGoogle Scholar
  19. Lin ZF, Li SS, Lin GZ, Guo JY (1988) Relation between H2O2 accumulation and membrane lipid peroxidation in aging leaves and chloroplasts. Acta Phytophysiol Sinica 14(1):16–22 in chinGoogle Scholar
  20. Liu C, Hong FS, Wang L, Zheng L (2004a) The effect of Nd3+ on photosynthesis of spinach. J Rare Earths 22(2):306–310Google Scholar
  21. Liu C, Hong FS, Zheng L, Tang P, Wang ZG (2004b) Effects of rare earth elements on vigor enhancement of aged spinach seeds. J Rare Earths 22(4):547–552Google Scholar
  22. Marschner H, Cakmak I (1989) High light intensity enhances chlorosis and necrosis in leaves of zinc, potassium and magnesium deficient bean (Phaseolus vulgaris) plants. Plant Physiol 134:308–315Google Scholar
  23. Murata N (1969) Control of excitation transfer in photosynthesis II. Magnesium ion-dependent distribution of excitation energy between two pigment systems in spinach chloroplasts. J Biochim Biophys Acta 189:171–181. doi: 10.1016/0005-2728(69)90045-0 CrossRefGoogle Scholar
  24. Orit S (2002) Magnesium transport and function in plants: the tip of the iceberg. Biometals 15:309–323Google Scholar
  25. Poller A, Otter T, Kobsbm JA (1994) Effect of magnesium-deficiency on antioxidative systems in needles of Norway spruce grown with different ratios of nitrate and ammonium as nitrogen sources. New Phytol 128:621–628. doi: 10.1111/j.1469-8137.1994.tb04026.x CrossRefGoogle Scholar
  26. Prasad TK (1997) Role of catalase in inducing chilling tolerance in pre-emergent maize seedlings. Plant Physiol 114:1369–1376PubMedGoogle Scholar
  27. Reuveni R, Shimoni M, Karchi Z, Kuc J (1992) Peroxidase activity as a biochemical marker for resistance of muskmelon (Cucumis melo) to seudoperno spora cubensis. Phytopathol 82:749–753. doi: 10.1094/Phyto-82-749 CrossRefGoogle Scholar
  28. Rurainski HJ, Mader G (1977) Regulation of the hill reaction by cation and its abolishment by uncouplers. Biochim Biophys Acta 461:489–499. doi: 10.1016/0005-2728(77)90235-3 CrossRefPubMedGoogle Scholar
  29. Shanghai Plant Physiology Society (ed) (1999) Experimental guide of modern plant physiology, vol 75. Science Press, Beijing, pp 302–303 in chinGoogle Scholar
  30. Wang H, Chu TD (1998) Effect of Mg deficiency on plasma membrane permeability, membrane lipid peroxidation and endogenous oxygen radicals scavenging enzymes systems in common bean. Plant Nutr Fertil Sci 4(4):368–392 in chinGoogle Scholar
  31. Wang AG, Luo GH (1990) Relationship between superoxide free radicals of plant and hydroxyl-ammonia quantitative reaction. Plant Physiol Commun 26(6):55–59 in chinGoogle Scholar
  32. Wang JS, Guo CR, Cheng YX (1997) Mechanism of cerium ion clearing superoxide radical. J Chin Rare Earth Soc 15(2):151–154 in chinGoogle Scholar
  33. Yang F, Ma ZN, Liu C, Wu C, Zhou J, Gao FQ, Hong FS (2005) Effects of Ce3+ on chloroplast senescence of spinach under light. J Rare Earths 22(4):480–485Google Scholar

Copyright information

© Springer Science+Business Media, LLC. 2009

Authors and Affiliations

  • Yuguan Ze
    • 1
  • Sitao Yin
    • 1
  • Zhe Ji
    • 1
  • Luyang Luo
    • 1
  • Chao Liu
    • 1
  • Fashui Hong
    • 1
  1. 1.Medical College of Soochow UniversitySuzhouPeople’s Republic of China

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