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Hypercholesterolemia and LDL receptor mRNA expression: modulation by selenium supplementation

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Abstract

Selenium (Se) status has been associated with cardiovascular disorders. Present study was aimed to elucidate the protective role of Se supplementation on LDL receptor (LDL-R) activity as well as mRNA expression during experimental hypercholesterolemia in SD male rats. Animals were fed 0.2 and 1 ppm Se supplemented control diet as well as 2% cholesterol supplemented diet for 3 months. LDL-R activity was measured in-vivo by injecting radiolabeled LDL to rats and decrease in counts per minute with time was taken as a measure of LDL clearance and in turn LDL-R activity. LDL-R mRNA expression was studied by RT-PCR. LDL-R activity and mRNA expression decreased significantly on 2% cholesterol supplemented diet feeding. On 1 ppm Se supplementation LDL-R activity as well as mRNA expression increased significantly. Present results demonstrate that Se supplementation upto 1 ppm is responsible for up regulation of LDL-R activity as well as mRNA expression, during hypercholesterolemia. These findings highlight the therapeutic potential of Se supplementation in lipid metabolism.

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  1. Department of Biophysics, Punjab University, Chandigarh, 160014, India

    Sanjiv Dhingra & Mohinder P. Bansal

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  1. Sanjiv Dhingra
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  2. Mohinder P. Bansal
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Correspondence to Mohinder P. Bansal.

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Dhingra, S., Bansal, M.P. Hypercholesterolemia and LDL receptor mRNA expression: modulation by selenium supplementation. Biometals 19, 493–501 (2006). https://doi.org/10.1007/s10534-005-5393-z

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