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Biometals

, Volume 18, Issue 3, pp 213–220 | Cite as

Alterations in the lipid content of pituitary gland and serum prolactin and growth hormone in cadmium treated rats

  • Ana María Calderoni
  • Liliana Oliveros
  • Graciela Jahn
  • Rosa Anton
  • Juan Luco
  • María Sofía Giménez
Article

Abstract

The present study was undertaken to assess whether chronic exposition to cadmium (Cd, 0.133 mM per liter for 2 months) through drinking water may affect the lipid contents in the pituitary anterior lobe (PAL) of adult male Wistar rats. As compared to metal non-exposed controls, PALs exposed to cadmium showed an increase in total phospholipid contents, which was associated to an increase of the incorporation of [1−14C]-methyl choline into phosphatidylcholine and of [U−14C]-glucose into total phospholipids. The incorporation of [1−14C]-methyl choline into sphingomyelin was not changed. Incorporation of [1−14C]-acetate into total fatty acids also increased but incorporation of [1−14C]-acetate into cholesterol did not change. The activity of phospholipase D decreased both in PALs from Cd exposed rats and in PAL dispersed cells treated with Cd in the culture medium from Cd non-exposed rats. In PALS from Cd exposed rats, a decrease of serum prolactin and growth hormone concentrations was determined. The results shown that cadmium modifies the lipid contents of pituitary gland and directly or indirectly the levels of prolactin and growth hormone in serum.

Keywords

cadmium pituitary gland lipids phospholipids prolactin growth hormone 

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References

  1. Abell, LL, Levy, BB, Brodie, BB, Kendal, FE. 1952Simplified methods for the stimation of total cholesterol in serum and demostration of its specifityJ Biol Chem195357366PubMedGoogle Scholar
  2. Arriba Zerpa, GA, Guido, ME, Busolino, DF, Pasquare, SJ, Castagnet, PI, Giusto, NM, Caputto, BL. 1999Light activates retina ganglion cell lysophosphaidic acid acyltransferase and phosphatidic acid phosphatase by a c-fos-dependent mechanismJ Neurochem7312281235CrossRefPubMedGoogle Scholar
  3. Athenstaedt, K, Daum, G. 1999Phosphatidic acid, a key intermediate in lipid metabolismEur J Biochem26116CrossRefGoogle Scholar
  4. Benoff, S, Jacob, A, Hurley, IR. 2000Male infertility and environmental exposure to lead and cadmiumHum Reprod Update6107121CrossRefPubMedGoogle Scholar
  5. Beyersmann, B, Hechtenberg, S. 1997Cadmium, gene regulation, and cellular signalling in mammalian cellsToxicol Appl Pharmacol144247261CrossRefPubMedGoogle Scholar
  6. Bussolino, DF, Guido, ME, Gil, GA, Boriolo, GA, Renner, ML, Grabois, VR, Conde, CB, Caputto, BL. 2001C-Fos associates with the endoplasmic reticulum and activates phospholipid metabolismFASEB15556558Google Scholar
  7. Chen, YG, Siddhanta, A, Austin, CD, Hammond, SM, Sung, TG, Frohman, MA, Morris, AJ, Shields, D. 1997Phospholipase D stimulates release of nascent secretory vesicles from trans-golgi networkJ Cell Biol138495504CrossRefPubMedGoogle Scholar
  8. Crawford JK, Luoma SN. 1993 Guidelines for Studies of Contaminants in Biological Tissues for the National Water-Quality Assessment Program. USGS Open-File Report 92-494. U.S. Geological Survey, Lemoyne, PA.Google Scholar
  9. Diamond, GL, Thayer, WC, Choudhury, H. 2003Pharmacokinetics pharmacodynamics (PK/PD) modeling of risks of kidney toxicity from exposure to cadmium: estimates of dietary risks in the U.S. populationJ Toxicol Environ Health6621412164CrossRefGoogle Scholar
  10. Exton, JH. 1994Phosphatidylcholine breakdown and signal transductionBiochim Biophys Acta1212926942Google Scholar
  11. Gulati, S, Gill, KD, Nath, R. 1987Effect of cadmium on lipid metabolism of brain: in vivo incorporation of labelled acetate into lipidsPharmacol Toxicol60117119PubMedGoogle Scholar
  12. Hart, RP, Rose, CS, Hamer, RM. 1989Neuropsychological effects of occupational exposure to cadmiumJ Clin Exp Neuropsychol11933943PubMedGoogle Scholar
  13. Kumar, R, Agarwal, AK, Seth, PK. 1996Oxidative stress-mediated neurotoxicity of cadmiumToxicol Lett896569CrossRefPubMedGoogle Scholar
  14. Lafuente, A, Cano, P, Esquifino, A. 2003Are cadmium effects on plasma gonadotropins, prolactin, ACTH, GH and TSH levels, dose-dependent?Biometals16243250CrossRefPubMedGoogle Scholar
  15. Lafuente, A, Marquez, M, Perez-Lorenzo, M, Pazo, D, Esquifino, AI. 2001Cadmium effects on hypothalamic-pituitary-testicular axis in male ratsExp Biol Med226605611Google Scholar
  16. Li, M, Kondo, T, Zhao, QL, Li, FJ, Tanabe, K, Arai, Y, Zhou, ZC, Kasuya, M. 2000Apoptosis induced by cadmium in human lymphoma U937 cells through Ca2+-calpain and caspase-mitochondria-dependent pathwaysJ Biol Chem2753970239709CrossRefPubMedGoogle Scholar
  17. Liscovitch, M, Czarny, M, Flucci, G, Tang, X. 2000Phospholipase D: molecular and cell biology of a novel gen familyBiochem J345401415CrossRefPubMedGoogle Scholar
  18. Martin, A, Gómez-Muñoz, A, Waggoner, DW, Stone, J, Brindley, DN. 1993Decreased activities of phosphatidate phosphohydrolase and phospholipase D in rats and tyrosine kinase (fps) transformed fibroblastsJ Biol Chem2682392423932PubMedGoogle Scholar
  19. Méndez-Armenta, M, Villeda-Hernández, J, Barroso-Moguel, R, Nava-Ruíz, C, Jiménez- Capdeville, ME, Ríos, C. 2003Brain regional lipid peroxidation and metallothionein levels of developing rats exposed to cadmium and dexamethasoneToxicol Lett144151157CrossRefPubMedGoogle Scholar
  20. Misra, UK, Gawdi, G, Pizzo, CV. 2003Induction of mitogenic signalling in the ILN prostate cell line on exposure to submicromolar concentrations of cadmiumCell Signal1510591070CrossRefPubMedGoogle Scholar
  21. Poliandri, AH, Cabilla, JP, Velardez, MO, Bodo, CC, Duvilanski, BH. 2003Cadmium induces apoptosis in anterior pituitary cells that can be reversed by treatment with antioxidantsToxicol Appl Pharmacol1901724CrossRefPubMedGoogle Scholar
  22. Ramirez, DC, Giménez, MS. 2000Varied protocols of cadmium exposure produce different effects on nitric oxide production in macrophagesToxicology1466172CrossRefPubMedGoogle Scholar
  23. Ramirez, DC, Giménez, MS. 2002Lipid modification in mouse peritoneal macrophages alter chronic cadmium exposureToxicology172112CrossRefPubMedGoogle Scholar
  24. Ramirez, DC, Giménez, MS. 2003Induction of redox changes, inducible nitric oxide synthase and cyclooxygenase 2 by chronic cadmium exposure in mouse peritoneal macrophagesToxicol Lett145121132CrossRefPubMedGoogle Scholar
  25. Rauser, G, Fluster, S, Yamamoto, A. 1970Two dimensional thin-layer chromatographic separation of polar lipid and determination of phospholipid analysis of spotsLipids5494496PubMedGoogle Scholar
  26. Roth, MG. 1999Lipid regulators of membrane traffic through the Golgi complexTrends Cell Biol9174179CrossRefPubMedGoogle Scholar
  27. Saderholm, MJ, Higtower, KE, Firke, CA. 2000Role of metals in the reaction catalyzed by protein farnesyltransferaseBiochemistry391239812405CrossRefPubMedGoogle Scholar
  28. Senogles, SE. 2000D2s dopamine receptor stimulates phospholipase D activity: a novel signaling pathway for dopamineMol Pharmacol58455462PubMedGoogle Scholar
  29. Shukla, GS, Husain, T, Srivastava, RS, Chandra, SV. 1989Glutathione peroxidase and catalase in liver, kidney, testis and brain regions of rats following cadmium exposure and subsequent withdrawalInd Health295969Google Scholar
  30. Sidhanta, A, Shield, D. 1998Secretory vesicle budding from the trans-golgi network is mediated by phosphatidic acid levelsJ Biol Chem2731799517998CrossRefPubMedGoogle Scholar
  31. Sidhanta, A, Jonathan, MB, Shield, D. 2000Inhibition of phosphatidic acid synthesis alters the structure of the golgi apparatus and inhibits secretion in endocrine cellsJ Biol Chem161202312031CrossRefGoogle Scholar
  32. Sorahan, T, Esmen, NA. 2004Lung cancer mortality in UK nickel-cadmium battery workersOccup Environ Med6108116CrossRefGoogle Scholar
  33. Sugita, M, Tsuchiya, K 1995Estimation of variation among individuals of biological half time of cadmium calculated from accumulation dataEnviron Res683137CrossRefPubMedGoogle Scholar
  34. Trzcinka-Ochocka, M, Jakubowski, M, Halatek, T, Razniewska, G. 2002Reversibility of microproteinuria in nickel-cadmium battery workers after removal from exposureInt Arch Occup Environ Health75S101S106CrossRefPubMedGoogle Scholar
  35. Velardez, MO, Benitez, AH, Cabilla, JP, Bodo, CC, Duvilanski, BH. 2003Nitric oxide decreases the production of inositol phosphates stimulated by angiotensin II and thyrotropin-releasing hormone in anterior pituitary cellsEur J Endocrinol1488997CrossRefPubMedGoogle Scholar
  36. Waalkes, MP. 2003Cadmium carcinogenesisMutat Res533107120PubMedGoogle Scholar
  37. Zack, B, Moss, N, Boyle, AS, Zlatkis, A. 1954Reactions of certain unsaturated steroids with acid iron reagentAnal Chem26776777CrossRefGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Ana María Calderoni
    • 1
  • Liliana Oliveros
    • 1
  • Graciela Jahn
    • 2
  • Rosa Anton
    • 3
  • Juan Luco
    • 4
  • María Sofía Giménez
    • 1
  1. 1.Laboratorio de Química Biológica, Facultad de Química, Bioquímica y FarmaciaUniversidad Nacional de San LuisSan Luis
  2. 2.CRICYT, CONICETIHEM Universidad Nacional de Cuyo e INBECUMendoza
  3. 3.Laboratorio de Química Analítica y, Facultad de Química, Bioquímica y FarmaciaUniversidad Nacional de San LuisSan LuisArgentina
  4. 4.Laboratorio de Alimentos, Facultad de Química, Bioquímica y FarmaciaUniversidad Nacional de San LuisSan LuisArgentina

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