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Biometals

, Volume 18, Issue 2, pp 171–177 | Cite as

Stability, protein binding and thiol interaction studies on [2-acetoxy-(2-propynyl)benzoate]hexacarbonyldicobalt

  • Ingo Ott
  • Ronald Gust
Article

Abstract

Cobalt–alkyne complexes are a new class of potent cytotoxic drugs. The lead compound [2-acetoxy-(2-propynyl)benzoate]hexacarbonyldicobalt (Co-ASS) showed high effects on several human cancer cell lines. In order to evaluate further the in-vitro properties and reactivity of this substance we performed stability and protein binding studies and investigated the interaction of this complex with 1,2-ethanedithiol, L-cysteine and glutathione. UV–Vis, HPLC and AAS studies showed that the compound was sufficiently stable under in-vitro conditions. Binding to human serum albumin increased from approximately 25% at the beginning to over 50% after 48 h of incubation as determined by ethanol preciptation and size exclusion chromatography. The interaction with thiols resulted in disulfide bond formation of the thiols.

Keywords

cobalt complexes glutathione protein binding stability 

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Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Institute of PharmacyFree University of BerlinBerlin

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