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Biometals

, Volume 17, Issue 6, pp 707–713 | Cite as

Zinc protects against arsenic-induced apoptosis in a neuronal cell line, measured by DEVD-caspase activity

  • Austin Graham Milton
  • Peter David Zalewski
  • Ranjit Nihal Ratnaike
Article

Abstract

Acute and chronic arsenic exposure results in toxicity in humans and causes many neurological and other manifestations. For the first time the present study reports that zinc decreases arsenic-induced apoptosis and also confirms a single report of apoptosis induced by arsenic in a neuronal cell line. Apoptosis measured by DEVD-caspase activity peaked between 10 μM and 20 μM of arsenic trioxide. Higher concentrations of arsenic up to 40 μM caused increasing cell death with diminishing DEVD-caspase activity. The beneficial effect of zinc was proportional to its concentration with a significant decrease in arsenic-induced DEVD-caspase activity at 50 μM and 75 μM zinc (P<0.05). This finding may be of therapeutic benefit in people suffering from chronic exposure to arsenic from natural sources, a global problem especially relevant to millions of people on the Indian subcontinent.

arsenic trioxide 2.3D mouse neuroepithelial cells DEVD-caspase zinc sulfate 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Austin Graham Milton
    • 1
  • Peter David Zalewski
    • 1
  • Ranjit Nihal Ratnaike
    • 1
  1. 1.Department of MedicineThe Queen Elizabeth HospitalWoodvilleSouth Australia

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