Effects of sulfur-metabolizing bacterial community diversity on H2S emission behavior in landfills with different operation modes
Hydrogen sulfide (H2S) is one of the major contributors to offensive odors from landfills, and its concentration differs under different operation modes. This study examined the distribution of H2S emission from different landfill depths under different operation modes (anaerobic, semi-aerobic, semi-aerobic transformation, and the three operation modes with additional leachate recirculation). The microbial community (especially the sulfur-metabolizing bacterial community) was investigated using high-throughput sequencing technology. The results showed that the semi-aerobic mode could substantially lower the risks of H2S pollution in landfills, which might be because of the difference in biological processes related to sulfur metabolism driven by functional microbes. A myriad of factors are responsible for mutually shaping the sulfur-metabolizing bacterial community composition in landfills that might subsequently affect the behavior of H2S emission in landfills. The differences in abundance of the genera Acinetobacter and Paracoccus (phylum Proteobacteria) caused by environmental factors might explain the differences in H2S emission. H2S odor control could be realized if the related functional microbe diversity can be influenced by adjustments to landfill operation.
KeywordsH2S Landfill Operation modes Sulfur-metabolizing bacteria High-throughput sequencing
This work was financially supported by the National Natural Science Foundation of China (41471408 and 41101453) and the Natural Science Foundation of Zhejiang Province (LY14D010001).
- APHA (1999) Standard methods for the examination of water and wastewater. APHA, Washington, DCGoogle Scholar
- Lu RK (1999) Agriculture chemical analysis of soil. China Agricultural Scientech Press, Beijing (in Chinese)Google Scholar
- Masoud W, Takamiya M, Vogensen FK, Lillevang S, Al-Soud WA, Sørensen SJ, Jakobsen M (2011) Characterization of bacterial populations in Danish raw milk cheeses made with different starter cultures by denaturating gradient gel electrophoresis and pyrosequencing. Int Dairy J 21:142–148CrossRefGoogle Scholar
- Michaelis W, Seifert R, Nauhaus K, Treude T, Thiel V, Blumenberg M, Knittel K, Gieseke A, Peterknecht K, Pape T, Boetius A, Amann R, Jorgensen BB, Widdel F, Peckmann J, Pimenov NV, Gulin MB (2002) Microbial reefs in the Black Sea fueled by anaerobic oxidation of methane. Science 297:1013–1015CrossRefPubMedGoogle Scholar
- Qiu CL, Liu JX, Peng ML (1992) Amendment method for sulfide content of soil. Public Heath 8:549–550Google Scholar