Quantitative and functional dynamics of Dehalococcoides spp. and its tceA and vcrA genes under TCE exposure
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This study aimed at monitoring the dynamics of phylogenetic and catabolic genes of a dechlorinating enrichment culture before, during, and after complete dechlorination of chlorinated compounds. More specifically, the effect of 40 μM trichloroethene (TCE) and 5.6 mM lactate on the gene abundance and activity of an enrichment culture was investigated for 40 days. Although tceA and vcrA gene copy numbers were relatively stable in DNA extracts over time, tceA and vcrA mRNA abundances were up-regulated from undetectable levels to 2.96 × and 6.33 × 104 transcripts/mL, respectively, only after exposure to TCE and lactate. While tceA gene transcripts decreased over time with TCE dechlorination, the vcrA gene was expressed steadily even when the concentration of vinyl chloride was at undetectable levels. In addition, ratios between catabolic and phylogenetic genes indicated that tceA and vcrA gene carrying organisms dechlorinated TCE and its produced daughter products, while vcrA gene was mainly responsible for the dechlorination of the lower VC concentrations in a later stage of degradation.
KeywordsDehalococcoides spp. RDase genes ATP activity RNA Dynamics
The research has received funding from the European Community’s Seventh Framework Program FP7/2007-2013 under Grant agreement no 212683 (GOODWATER). Contributions of Wietse Heylen, Queenie Simons and Yamini Satyawali are appreciated.
- Hamonts K, Kuhn T, Maesen M, Bronders J, Lookman R, Kalka H, Diels L, Meckenstock RU, Springael D, Dejonghe W (2009) Factors determining the attenuation of chlorinated aliphatic hydrocarbons in eutrophic river sediment impacted by discharging polluted groundwater. Environ Sci Technol 43:5270–5275PubMedCrossRefGoogle Scholar
- Hendrickx B, Dejonghe W, Boënne W, Brennerova M, Cernik M, Lederer T, Bucheli-Witschel M, Bastiaens L, Verstraete W, Top EM, Diels L, Springael D (2005) Dynamics of an oligotrophic bacterial aquifer community during contact with a groundwater plume contaminated with benzene, toluene, ethylbenzene, and xylenes: an in situ mesocosm study. Appl Environ Microbiol 71:3815–3825PubMedCentralPubMedCrossRefGoogle Scholar
- Karl DM (1993) Total microbial biomass estimation derived from the measurement of particulate adenosine-5′-triphospate. In: Kemp PF, Sherr BF, Sherr EB, Cole JJ (eds) Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Roton, p 359–367Google Scholar
- Müller JA, Rosner BM, von Abendroth G, Meshulam-Simon G, McCarty PL, Spormann AM (2004) Molecular identification of the catabolic vinyl chloride reductase from Dehalococcoides sp. strain VS and its environmental distribution. Appl Environ Microbiol 70(8):4880–4888PubMedCentralPubMedCrossRefGoogle Scholar
- Ritalahti KM, Hatt JK, Lugmayr V, Henn K, Petrovskis EA, Ogles DM, Davis GA, Yeager CM, Lebrón CA, Löffler FE (2010) Comparing on-site to off-site biomass collection for Dehalococcoides biomarker gene quantification to predict in situ chlorinated ethene detoxification potential. Environ Sci Technol 44(13):5127–5133PubMedCrossRefGoogle Scholar
- Scheutz C, Durant ND, Dennis P, Hansen MH, Jørgensen T, Jakobsen R, Cox EE, Bjerg PL (2008) Concurrent ethene generation and growth of Dehalococcoides containing vinyl chloride reductive dehalogenase genes during an enhanced reductive dechlorination field demonstration. Environ Sci Technol 42:9302–9309PubMedCrossRefGoogle Scholar
- SERDP & ESTCP Expert Panel Workshop: Research & Development Needs for the Environmental Remediation Application of Molecular Biological Tools, 2005Google Scholar
- van der Zaan B, Hannes F, Hoekstra N, Rijnaarts H, de Vos WM, Smidt H, Gerritse J (2010) Correlation of Dehalococcoides 16S rRNA and chloroethene-reductive dehalogenase genes with geochemical conditions in chloroethene-contaminated groundwater. Appl Environ Microbiol 76(3):843–850PubMedCentralPubMedCrossRefGoogle Scholar