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Metabolomic and proteomic insights into carbaryl catabolism by Burkholderia sp. C3 and degradation of ten N-methylcarbamates

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Abstract

Burkholderia sp. C3, an efficient polycyclic aromatic hydrocarbon degrader, can utilize nine of the ten N-methylcarbamate insecticides including carbaryl as a sole source of carbon. Rapid hydrolysis of carbaryl in C3 is followed by slow catabolism of the resulting 1-naphthol. This study focused on metabolomes and proteomes in C3 cells utilizing carbaryl in comparison to those using glucose or nutrient broth. Sixty of the 867 detected proteins were involved in primary metabolism, adaptive sensing and regulation, transport, stress response, and detoxification. Among the 41 proteins expressed in response to carbaryl were formate dehydrogenase, aldehyde-alcohol dehydrogenase and ethanolamine utilization protein involved in one carbon metabolism. Acetate kinase and phasin were 2 of the 19 proteins that were not detected in carbaryl-supported C3 cells, but detected in glucose-supported C3 cells. Down-production of phasin and polyhydroxyalkanoates in carbaryl-supported C3 cells suggests insufficient carbon sources and lower levels of primary metabolites to maintain an ordinary level of metabolism. Differential metabolomes (~196 identified polar metabolites) showed up-production of metabolites in pentose phosphate pathways and metabolisms of cysteine, cystine and some other amino acids, disaccharides and nicotinate, in contract to down-production of most of the other amino acids and hexoses. The proteomic and metabolomic analyses showed that carbaryl-supported C3 cells experienced strong toxic effects, oxidative stresses, DNA/RNA damages and carbon nutrient deficiency.

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Acknowledgments

This work was supported in part with US-EPA award no. 989512-01-1, US ONR HEET award N00014-09-1-0709, and grants from the National Institute on Minority Health and Health Disparities (8 G12 MD007601-26).

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  1. Department of Molecular Biosciences and Bioengineering, University of Hawaii, 1955 East-West Road, Honolulu, HI, 96822, USA

    Jong-Su Seo, Young-Soo Keum & Qing X. Li

  2. Environmental Toxicology Research Center, Korea Institute of Toxicology, 100 Jangdong, Yuseonggu, Daejeon, 305-343, South Korea

    Jong-Su Seo

  3. Department of Molecular Biotechnology, KonKuk University, Seoul, 143-701, South Korea

    Young-Soo Keum

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  1. Jong-Su Seo
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Correspondence to Qing X. Li.

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Jong-Su Seo and Young-Soo Keum equally contributed to this study.

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Seo, JS., Keum, YS. & Li, Q.X. Metabolomic and proteomic insights into carbaryl catabolism by Burkholderia sp. C3 and degradation of ten N-methylcarbamates. Biodegradation 24, 795–811 (2013). https://doi.org/10.1007/s10532-013-9629-2

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