A global proteome study of Mycobacterium gilvum PYR-GCK grown on pyrene and glucose reveals the activation of glyoxylate, shikimate and gluconeogenetic pathways through the central carbon metabolism highway
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Various hydrocarbons have been released into the environment as a result of industrialization. An effective way of removing these materials without further environmental contamination is microbial bioremediation. Mycobacterium gilvum PYR-GCK, a bacteria isolated from a PAH polluted estuary, was studied using comparative shotgun proteomics to gain insight on its molecular activity while using pyrene and glucose as sole carbon and energy sources. Based on annotated genomic information, a confirmation analysis was first performed to confirm its pyrene degradation activity, using gas chromatography–mass spectrometry technology. One dimensional gel electrophoresis and liquid chromatography–mass spectrometry technologies employed in the proteomics analysis revealed the expression of pyrene degrading gene products along with upregulated expression of proteins functioning in the glyoxylate and shikimate pathways, in the pyrene-induced cells. The study also revealed the pathway of pyrene degraded intermediates, via partial gluconeogenesis, into the pentose phosphate pathway to produce precursors for nucleotides and amino acids biosynthesis.
KeywordsPolycyclic aromatic hydrocarbons Proteomics Shikimate Glyoxylate Mycobacterium gilvum PYR-GCK
This work was supported by the Development of Biohydrogen Production Technology using Hyperthermophilic Archaea program and the Marine and Extreme Genome Research Center program of the Ministry of Land, Transport, and Maritime Affairs, a KBSI grant (K32403) to Y.H.C., and the National Research Foundation of Korea Grant funded by the Korean Government (No. 2012-0009212 to Y.G.C).
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