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Biodegradation

, Volume 22, Issue 2, pp 431–444 | Cite as

Impacts of microbial community composition on isotope fractionation during reductive dechlorination of tetrachloroethylene

  • Yiran Dong
  • Elizabeth C. Butler
  • R. Paul Philp
  • Lee R. Krumholz
Original Paper

Abstract

Isotope fractionation has been used with increasing frequency as a tool to quantify degradation of chlorinated aliphatic pollutants in the environment. The objective of this research was to determine if the electron donor present in enrichment cultures prepared from uncontaminated sediments influenced the extent of isotope fractionation of tetrachloroethylene (PCE), either directly, or through its influence on microbial community composition. Two PCE-degrading enrichment cultures were prepared from Duck Pond (DP) sediment and were incubated with formate (DPF) or H2 (DPH) as electron donor. DPF and DPH were significantly different in both product distribution and extent of isotope fractionation. Chemical and isotope analyses indicated that electron donors did not directly affect the product distribution or the extent of isotope fractionation for PCE reductive dechlorination. Instead, restriction fragment length polymorphism (RFLP) and sequence analysis of the 16S rRNA clone libraries of DPF and DPH identified distinct microbial communities in each enrichment culture, suggesting that differences in microbial communities were responsible for distinct product distributions and isotope fractionation between the two cultures. A dominant species identified only in DPH was closely related to known dehalogenating species (Sulfurospirillum multivorans and Sulfurospirillum halorespirans) and may be responsible for PCE degradation in DPH. Our study suggests that different dechlorinators exist at the same site and can be preferentially stimulated by different electron donors, especially over the long-term (i.e., years), typical of in-situ ground water remediation.

Keywords

Tetrachloroethylene Reductive dechlorination Microbial community composition Isotope fractionation Electron donors 

Notes

Acknowledgments

We thank Tomasz Kuder and Janel McMahon from the School of Geology and Geophysics at the University of Oklahoma (OU) for assistance in isotope analyses. Funding was provided by the OU School of Civil Engineering and Environmental Science and Department of Botany and Microbiology. The OU Graduate College also provided funding through a Robberson Research Grant to Y. D.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Yiran Dong
    • 1
    • 2
  • Elizabeth C. Butler
    • 2
  • R. Paul Philp
    • 3
  • Lee R. Krumholz
    • 4
  1. 1.Energy and Bioscience InstituteUniversity of Illinois-Urbana ChampaignUrbanaUSA
  2. 2.School of Civil Engineering and Environmental ScienceUniversity of OklahomaNormanUSA
  3. 3.School of Geology and GeophysicsUniversity of OklahomaNormanUSA
  4. 4.Department of Botany and MicrobiologyUniversity of OklahomaNormanUSA

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