, Volume 22, Issue 1, pp 13–23 | Cite as

Multisubstrate kinetics of PAH mixture biodegradation: analysis in the double-logarithmic plot

Original Paper


The proposed method of kinetic analysis of aqueous-phase biodegradation of polycyclic aromatic hydrocarbons (PAH) mixture presupposes representation of kinetic curves for each pair of mixture components, S x and S y , in double-logarithmic coordinates (ln S x ; ln S y ). If PAH mixture conversion corresponds to the multisubstrate model with a common active site, then the graphs in double-logarithmic coordinates are straight lines with the angular coefficients equal to the ratio of respective first-order rate constants \( k_{x}^{y} = {\frac{{V_{y} K_{x} }}{{K_{y} V_{x} }}} \), where K x and K y are half-saturation constants, V x and V y are the maximum conversion rates for substrates S x and S y ; the graph slope does not depend on any concentrations and remains constant during the change of reaction rates as a result of inhibition, induction/inactivation of enzymes or biomass growth. The formulated method has been used to analyze PAH mixture conversion by the culture of Sphingomonas sp. VKM B-2434. It has been shown that this process does not satisfy the multisubstrate model with a single active site. The results suggest that the strain VKM B-2434 contains at least two dioxygenases of different substrate specificity: one enzyme converts phenanthrene and fluoranthene and the other converts acenaphthene and acenaphthylene. The ratios of first-order rate constants have been obtained for these pairs of substrates.


Multisubstrate kinetics PAHs Sphingomonas sp. VKM B-2434 


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms RASPushchinoRussia

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