Abstract
In the present study, a new bacterial strain isolated from activated sludge has been identified as Lysinibacillus sphaericus based on its morphology, physiochemical properties, and the results of 16S ribosomal RNA (rRNA) gene sequence analysis. This new bacterial strain uses ethanethiol as both carbon source and energy source. The key factors for controlling the degradation efficiency of ethanethiol by this strain were found to be initial ethanethiol concentration, temperature, and pH value of solutions. Under the optimized conditions, as well as 4 mg l−1 ethanethiol, 30°C, and pH = 7.0, almost 100% ethanethiol can be degraded within 96 h and sulfate as a final product was detected in aqueous medium. The degradation reaction of ethanethiol over this newly isolated strain can be described by pseudo first-order equation in which the maximum degradation rate constant K and the minimum half-life were respectively calculated to be 0.0308 h−1 and 22.5 h under the optimal conditions.
Similar content being viewed by others
References
Arutchelvan V, Kanakasabai V, Nagarajan S, Muralikrishnan V (2005) Isolation and identification of novel high strength phenol degrading bacterial strains from phenol-formaldehyde resin manufacturing industrial wastewater. J Hazard Mater 127:238–243
Burgess JE, Parsons SA, Stuetz RM (2001) Developments in odour control and waste gas treatment biotechnology: a review. Biotechnol Adv 19:35–63
Cheng XH, Peterkin E, Burlingame GA (2005) A study on volatile organic sulfide causes of odors at Philadelphia’s northeast water pollution control plant. Water Res 39:3781–3790
Cohen Y (2001) Biofiltration—the treatment of fluids by microorganisms immobilized into the filter bedding material: a review. Bioresour Technol 77:257–274
De Bo I, Heyman J, Vincke J, Verstraete W, Van Langenhove H (2003) Dimethyl sulfide removal from synthetic waste gas using a flat poly (dimethylsiloxane)-coated composite membrane bioreactor. Environ Sci Technol 37:4228–4234
Easter C, Quigley C, Burrowes P, Witherspoon J, Apgar D (2005) Odor and air emissions control using biotechnology for both collection and wastewater treatment systems. Chem Eng J 113:93–104
Goldberg JB, Ohman DE (1984) Cloning and expression in Pseudomonas aeruginosa of a gene involved in the production of alginate. J Bacteriol 158:1115–1121
Gostelow P, Parsons SA, Stuetz RM (2001) Odour measurements for sewage treatment works. Water Res 35:579–597
Ho KL, Chung YC, Lin YH, Tseng CP (2008a) Microbial populations analysis and field application of biofilter for the removal of volatile-sulfur compounds from swine wastewater treatment system. J Hazard Mater 152:580–588
Ho KL, Chung YC, Lin YH, Tseng CP (2008b) Biofiltration of trimethylamine, dimethylamine, and methylamine by immobilized Paracoccus sp. CP2 and Arthrobacter sp. CP1. Chemosphere 72:250–256
Hort C, Gracy S, Platel V, Moynault L (2009) Evaluation of sewage sludge and yard waste compost as a biofilter media for the removal of ammonia and volatile organic sulfur compounds (VOSCs). Chem Eng J 152:44–53
Hwang SCJ, Wu JY, Lin YH, Wen IC, Hou KY, He SY (2007) Optimal dimethyl sulfoxide biodegradation using activated sludge from a chemical plant. Process Biochem 42:1398–1405
Kolb B, Ettre LS (1997) Static headspace-gas chromatography: theory and practice, 1st edn. Wiley-VCH, New York
Luo J, Lindsey S (2006) The use of pine bark and natural zeolite as biofilter media to remove animal rendering process odours. Bioresour Technol 97:1461–1469
Mathur AK, Majumder CB (2008) Biofiltration and kinetic aspects of a biotrickling filter for the removal of paint solvent mixture laden air stream. J Hazard Mater 152:1027–1036
McNevin D, Barford J (2000) Biofiltration as an odour abatement strategy. Biochem Eng J 5:231–242
Muezzinoglu A (2003) A study of volatile organic sulfur emissions causing urban odors. Chemosphere 51:245–252
Nimmermark S (2004) Odour influence on well-being and health with specific focus on animal production emissions. Ann Agric Environ Med 11:163–173
Park SJ, Hirai M, Shoda M (1993) Treatment of exhaust gases from a night soil treatment plant by a combined deodorization system of activated carbon fabric reactor and peat biofilter inoculated with Thiobacillus thioparus DW44. J Ferment Bioeng 76:423–426
Rappert S, Muler R (2005) Odor compounds in waste gas emissions from agricultural operations and food industries. Waste Manag 25:887–907
Reardon KF, Mosteller DC, Bull Rogers JD (2000) Biodegradation kinetics of benzene, toluene, and phenol as single and mixed substrates for Pseudomonas putida F 1. Biotechnol Bioeng 69:385–400
Sercu B, Nunez D, Aroca G, Boon N, Verstraete W, Van Langenhove H (2005) Inoculation and start-up of a biotricking filter removing dimethyl sulfide. Chem Eng J 113:127–134
Shareefdeen Z, Herner B, Webb D, Verhaeghe L, Wilson S (2005) An odor predictive model for rendering applications. Chem Eng J 113:215–220
Sheridan BA, Curran TP, Dodd VA (2003) Biofiltration of n-butyric acid for the control of odour. Bioresour Technol 89:199–205
Sironi S, Capelli L, Cétola P, Del Rosso R, Grande MI (2007) Odour emission factors for assessment and prediction of Italian rendering plants odour impact. Chem Eng J 131:225–231
Smet E, Lens P, Van Langenhove H (1998) Treatment of waste gases contaminated with odorous sulfur compounds. Crit Rev Environ Sci Technol 28:89–117
Smet E, Langenhove HV, Philips G (1999) Dolomite limits acidification of a biofilter degrading dimethyl sulphide. Biodegradation 10:399–404
Smith NA, Kelly DP (1988) Isolation and physiological characterization of autotrophic sulphur bacteria oxidizing dimethyl disulphide as sole source of energy. J Gen Microbiol 134:1407
Takashi I, Tatsuro M, Tomoyuki N, Noboru T (2007) Degradation of dimethyl disulfide by Pseudomonas fluorescens Strain 76. Biosci Biotechnol Biochem 71:366–370
Tsang YF, Chua H, Sin SN, Chan SY (2008) Treatment of odorous volatile fatty acids using a biotrickling filter. Bioresour Technol 99:589–595
Yoon SH, Chai XS, Zhu JY, Li J, Malcolm EW (2001) In-digester reduction of organic sulfur compounds in kraft pulping. Adv Environ Res 5:91–98
Acknowledgements
This is contribution no. IS-1196 from GIGCAS. This work was financially supported by the Science and Technology Project of Guangdong Province, China (2007A032301002, 2006A36701002 and 2009B030400001), and The Combination Project of Production, Studying, and Research of Foshan City (2008A040). The authors also thank Mrs. Guoxia Zhang for her kind help for making Phylogenetic tree and Dr. Ningzhong Bao for his English polishing throughout the paper.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Wan, S., Li, G., An, T. et al. Biodegradation of ethanethiol in aqueous medium by a new Lysinibacillus sphaericus strain RG-1 isolated from activated sludge. Biodegradation 21, 1057–1066 (2010). https://doi.org/10.1007/s10532-010-9366-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10532-010-9366-8