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Biodegradation

, Volume 21, Issue 6, pp 1057–1066 | Cite as

Biodegradation of ethanethiol in aqueous medium by a new Lysinibacillus sphaericus strain RG-1 isolated from activated sludge

  • Shungang Wan
  • Guiying Li
  • Taicheng An
  • Bin Guo
  • Lei Sun
  • Lei Zu
  • Ailing Ren
Original Paper

Abstract

In the present study, a new bacterial strain isolated from activated sludge has been identified as Lysinibacillus sphaericus based on its morphology, physiochemical properties, and the results of 16S ribosomal RNA (rRNA) gene sequence analysis. This new bacterial strain uses ethanethiol as both carbon source and energy source. The key factors for controlling the degradation efficiency of ethanethiol by this strain were found to be initial ethanethiol concentration, temperature, and pH value of solutions. Under the optimized conditions, as well as 4 mg l−1 ethanethiol, 30°C, and pH = 7.0, almost 100% ethanethiol can be degraded within 96 h and sulfate as a final product was detected in aqueous medium. The degradation reaction of ethanethiol over this newly isolated strain can be described by pseudo first-order equation in which the maximum degradation rate constant K and the minimum half-life were respectively calculated to be 0.0308 h−1 and 22.5 h under the optimal conditions.

Keywords

Lysinibacillus sphaericus Identification Wastewater treatment Biodegradation Ethanethiol 

Notes

Acknowledgements

This is contribution no. IS-1196 from GIGCAS. This work was financially supported by the Science and Technology Project of Guangdong Province, China (2007A032301002, 2006A36701002 and 2009B030400001), and The Combination Project of Production, Studying, and Research of Foshan City (2008A040). The authors also thank Mrs. Guoxia Zhang for her kind help for making Phylogenetic tree and Dr. Ningzhong Bao for his English polishing throughout the paper.

References

  1. Arutchelvan V, Kanakasabai V, Nagarajan S, Muralikrishnan V (2005) Isolation and identification of novel high strength phenol degrading bacterial strains from phenol-formaldehyde resin manufacturing industrial wastewater. J Hazard Mater 127:238–243CrossRefPubMedGoogle Scholar
  2. Burgess JE, Parsons SA, Stuetz RM (2001) Developments in odour control and waste gas treatment biotechnology: a review. Biotechnol Adv 19:35–63CrossRefPubMedGoogle Scholar
  3. Cheng XH, Peterkin E, Burlingame GA (2005) A study on volatile organic sulfide causes of odors at Philadelphia’s northeast water pollution control plant. Water Res 39:3781–3790CrossRefPubMedGoogle Scholar
  4. Cohen Y (2001) Biofiltration—the treatment of fluids by microorganisms immobilized into the filter bedding material: a review. Bioresour Technol 77:257–274CrossRefPubMedGoogle Scholar
  5. De Bo I, Heyman J, Vincke J, Verstraete W, Van Langenhove H (2003) Dimethyl sulfide removal from synthetic waste gas using a flat poly (dimethylsiloxane)-coated composite membrane bioreactor. Environ Sci Technol 37:4228–4234CrossRefPubMedGoogle Scholar
  6. Easter C, Quigley C, Burrowes P, Witherspoon J, Apgar D (2005) Odor and air emissions control using biotechnology for both collection and wastewater treatment systems. Chem Eng J 113:93–104CrossRefGoogle Scholar
  7. Goldberg JB, Ohman DE (1984) Cloning and expression in Pseudomonas aeruginosa of a gene involved in the production of alginate. J Bacteriol 158:1115–1121PubMedGoogle Scholar
  8. Gostelow P, Parsons SA, Stuetz RM (2001) Odour measurements for sewage treatment works. Water Res 35:579–597CrossRefPubMedGoogle Scholar
  9. Ho KL, Chung YC, Lin YH, Tseng CP (2008a) Microbial populations analysis and field application of biofilter for the removal of volatile-sulfur compounds from swine wastewater treatment system. J Hazard Mater 152:580–588CrossRefPubMedGoogle Scholar
  10. Ho KL, Chung YC, Lin YH, Tseng CP (2008b) Biofiltration of trimethylamine, dimethylamine, and methylamine by immobilized Paracoccus sp. CP2 and Arthrobacter sp. CP1. Chemosphere 72:250–256CrossRefPubMedGoogle Scholar
  11. Hort C, Gracy S, Platel V, Moynault L (2009) Evaluation of sewage sludge and yard waste compost as a biofilter media for the removal of ammonia and volatile organic sulfur compounds (VOSCs). Chem Eng J 152:44–53CrossRefGoogle Scholar
  12. Hwang SCJ, Wu JY, Lin YH, Wen IC, Hou KY, He SY (2007) Optimal dimethyl sulfoxide biodegradation using activated sludge from a chemical plant. Process Biochem 42:1398–1405CrossRefGoogle Scholar
  13. Kolb B, Ettre LS (1997) Static headspace-gas chromatography: theory and practice, 1st edn. Wiley-VCH, New YorkGoogle Scholar
  14. Luo J, Lindsey S (2006) The use of pine bark and natural zeolite as biofilter media to remove animal rendering process odours. Bioresour Technol 97:1461–1469CrossRefPubMedGoogle Scholar
  15. Mathur AK, Majumder CB (2008) Biofiltration and kinetic aspects of a biotrickling filter for the removal of paint solvent mixture laden air stream. J Hazard Mater 152:1027–1036CrossRefPubMedGoogle Scholar
  16. McNevin D, Barford J (2000) Biofiltration as an odour abatement strategy. Biochem Eng J 5:231–242CrossRefPubMedGoogle Scholar
  17. Muezzinoglu A (2003) A study of volatile organic sulfur emissions causing urban odors. Chemosphere 51:245–252CrossRefPubMedGoogle Scholar
  18. Nimmermark S (2004) Odour influence on well-being and health with specific focus on animal production emissions. Ann Agric Environ Med 11:163–173PubMedGoogle Scholar
  19. Park SJ, Hirai M, Shoda M (1993) Treatment of exhaust gases from a night soil treatment plant by a combined deodorization system of activated carbon fabric reactor and peat biofilter inoculated with Thiobacillus thioparus DW44. J Ferment Bioeng 76:423–426CrossRefGoogle Scholar
  20. Rappert S, Muler R (2005) Odor compounds in waste gas emissions from agricultural operations and food industries. Waste Manag 25:887–907CrossRefPubMedGoogle Scholar
  21. Reardon KF, Mosteller DC, Bull Rogers JD (2000) Biodegradation kinetics of benzene, toluene, and phenol as single and mixed substrates for Pseudomonas putida F 1. Biotechnol Bioeng 69:385–400CrossRefPubMedGoogle Scholar
  22. Sercu B, Nunez D, Aroca G, Boon N, Verstraete W, Van Langenhove H (2005) Inoculation and start-up of a biotricking filter removing dimethyl sulfide. Chem Eng J 113:127–134CrossRefGoogle Scholar
  23. Shareefdeen Z, Herner B, Webb D, Verhaeghe L, Wilson S (2005) An odor predictive model for rendering applications. Chem Eng J 113:215–220CrossRefGoogle Scholar
  24. Sheridan BA, Curran TP, Dodd VA (2003) Biofiltration of n-butyric acid for the control of odour. Bioresour Technol 89:199–205CrossRefPubMedGoogle Scholar
  25. Sironi S, Capelli L, Cétola P, Del Rosso R, Grande MI (2007) Odour emission factors for assessment and prediction of Italian rendering plants odour impact. Chem Eng J 131:225–231CrossRefGoogle Scholar
  26. Smet E, Lens P, Van Langenhove H (1998) Treatment of waste gases contaminated with odorous sulfur compounds. Crit Rev Environ Sci Technol 28:89–117CrossRefGoogle Scholar
  27. Smet E, Langenhove HV, Philips G (1999) Dolomite limits acidification of a biofilter degrading dimethyl sulphide. Biodegradation 10:399–404CrossRefPubMedGoogle Scholar
  28. Smith NA, Kelly DP (1988) Isolation and physiological characterization of autotrophic sulphur bacteria oxidizing dimethyl disulphide as sole source of energy. J Gen Microbiol 134:1407Google Scholar
  29. Takashi I, Tatsuro M, Tomoyuki N, Noboru T (2007) Degradation of dimethyl disulfide by Pseudomonas fluorescens Strain 76. Biosci Biotechnol Biochem 71:366–370CrossRefGoogle Scholar
  30. Tsang YF, Chua H, Sin SN, Chan SY (2008) Treatment of odorous volatile fatty acids using a biotrickling filter. Bioresour Technol 99:589–595CrossRefPubMedGoogle Scholar
  31. Yoon SH, Chai XS, Zhu JY, Li J, Malcolm EW (2001) In-digester reduction of organic sulfur compounds in kraft pulping. Adv Environ Res 5:91–98CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.The State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Protection and Resources Utilization, Guangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhouChina
  2. 2.Colleges of Environmental Science and EngineeringHebei University of Science and TechnologyShijiazhuangChina
  3. 3.Graduate School of Chinese Academy of SciencesBeijingChina

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