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Biodegradation

, Volume 21, Issue 6, pp 915–921 | Cite as

para-Nitrophenol 4-monooxygenase and hydroxyquinol 1,2-dioxygenase catalyze sequential transformation of 4-nitrocatechol in Pseudomonas sp. strain WBC-3

  • Min Wei
  • Jun-Jie Zhang
  • Hong Liu
  • Ning-Yi Zhou
Original Paper

Abstract

Pseudomonas sp. strain WBC-3 utilizes para-nitrophenol (PNP) as a sole source of carbon, nitrogen and energy. PnpA (PNP 4-monooxygenase) and PnpB (para-benzoquinone reductase) were shown to be involved in the initial steps of PNP catabolism via hydroquinone. We demonstrated here that PnpA also catalyzed monooxygenation of 4-nitrocatechol (4-NC) to hydroxyquinol, probably via hydroxyquinone. It was the first time that a single-component PNP monooxygenase has been shown to catalyze this conversion. PnpG encoded by a gene located in the PNP degradation cluster was purified as a His-tagged protein and identified as a hydroxyquinol dioxygenase catalyzing a ring-cleavage reaction of hydroxyquinol. Although all the genes necessary for 4-NC metabolism seemed to be present in the PNP degradation cluster in strain WBC-3, it was unable to grow on 4-NC as a sole source of carbon, nitrogen and energy. This was apparently due to the substrate’s inability to trigger the expression of genes involved in degradation. Nevertheless, strain WBC-3 could completely degrade both PNP and 4-NC when PNP was used as the inducer, demonstrating its potential in bioremediation of the environment polluted by both 4-NC and PNP.

Keywords

4-Nitrocatechol Hydroxyquinol 1,2-dioxygenase para-Nitrophenol 4-monooxygenase Pseudomonas Transformation 

Notes

Acknowledgments

We acknowledge financial supports from the National High Technology Research and Development Program of China (2006AA10Z403) and National Natural Science Foundation of China (30570021).

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Min Wei
    • 1
  • Jun-Jie Zhang
    • 1
  • Hong Liu
    • 1
  • Ning-Yi Zhou
    • 1
  1. 1.State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of SciencesWuhanChina

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