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Biodegradation

, Volume 21, Issue 6, pp 889–901 | Cite as

Novel evidence of cytochrome P450-catalyzed oxidation of phenanthrene in Phanerochaete chrysosporium under ligninolytic conditions

  • Daliang Ning
  • Hui Wang
  • Chang Ding
  • Huijie Lu
Original Paper

Abstract

The presence of cytochrome P450 and P450-mediated phenanthrene oxidation in the white rot fungus Phanerochaete chrysosporium under ligninolytic condition was first demonstrated in this study. The carbon monoxide difference spectra indicated induction of P450 (130 pmol mg−1 in the microsomal fraction) by phenanthrene. The microsomal P450 degraded phenanthrene with a NADPH-dependent activity of 0.44 ± 0.02 min−1. One of major detectable metabolites of phenanthrene in the ligninolytic cultures and microsomal fractions was identified as phenanthrene trans-9,10-dihydrodiol. Piperonyl butoxide, a P450 inhibitor which had no effect on manganese peroxidase activity, significantly inhibited phenanthrene degradation and the trans-9,10-dihydrodiol formation in both intact cultures and microsomal fractions. Furthermore, phenanthrene was also efficiently degraded by the extracellular fraction with high manganese peroxidase activity. These results indicate important roles of both manganese peroxidase and cytochrome P450 in phenanthrene metabolism by ligninolytic P. chrysosporium.

Keywords

Cytochrome P450 Phanerochaete chrysosporium Polycyclic aromatic hydrocarbon Metabolism 

Abbreviations

ABTS

2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonate)

HPLC

High performance liquid chromatography

LiP

Lignin peroxidase

LN

Low nitrogen

MnP

Manganese-dependent peroxidase

P420

Cytochrome P420

P450

Cytochrome P450

PAHs

Polycyclic aromatic hydrocarbons

PB

Piperonyl butoxide

PDA

Potato dextrose agar

Notes

Acknowledgement

This work was supported by National Nature Science Foundation of China (Admission No. 30400012 and 20737001) and Special Fund of State Key Joint Laboratory of Environment Simulation and Pollution Control (Nos. 08Z02ESPCT and 09Y03ESPCT).

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© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.State Key Joint Laboratory on Environment Simulation and Pollution Control, Department of Environmental Science and EngineeringTsinghua UniversityBeijingPeople’s Republic of China

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