Abstract
In order to accelerate biodegradation of feather into more amino acids, the fermentation medium of feather-biodegrading Streptomyces fradiae Var S-221 was optimized in this paper. In the first optimization step, the effects of feather powder, beet molasses, (NH4)2SO4 and KH2PO4 on amino acids formation were evaluated by using full factorial design. The results showed that feather powder and (NH4)2SO4 had significant and positive effects on feather-biodegradation into amino acids. Then, the method of the steepest ascent was used to access the optimal region of the two significant factors. In the third step, the concentration of feather powder and (NH4)2SO4 were further optimized with central composite design and response surface analysis. As a result, the composition of the optimal medium for S. fradiae Var S-221 fermentation were as follows (g/100 ml): feather powder, 19.504; beet molasses, 4.0; (NH4)2SO4, 1.467; KH2PO4, 0.3; MgSO4, 0.15; FeSO4, 0.001; ZnSO4, 0.0001; and MnSO4, 0.0001. Using this optimal fermentation medium, the amino acids concentration was increased from 4.61 to 6.13 g/100 ml.
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Cai CG, Lou BG, Zheng XD (2008) Keratinase production and keratin degradation by a mutant strain of Bacillus subtilis. J Zhejiang Univ Sci B 9(1):60–67
Deydier E, Guilet R, Sarda S, Sharrock P (2005) Physical and chemical characterization of crude meat and bone meal combustion residue: “waste or raw material?”. J Hazard Mater 121:141–148
Gousterova A, Braikova D, Goshev I, Christov P, Tishinov K, Vasileva-Tonkova E, Haertle T, Nedkov P (2005) Degradation of keratin and collagen containing wastes by newly isolated thermoactinomycetes or by alkaline hydrolysis. Lett Appl Microbiol 40(5):335–340
Gupta R, Ramnani P (2006) Microbial keratinases and their perspective applications: an overview. Appl Microbiol Biotechnol 4:1–13
Hao XC, Yu XB, Yan ZY (2006) Optimization of the medium for the production of cellulase by the mutant Trichoderma reesei WX-112 using response surface methodology. Food Technol Biotechnol 44(1):89–94
Kojima M, Kanai M, Tominaga M, Kitazume S, Inoue A, Horikoshi K (2006) Isolation and characterization of a feather-degrading enzyme from Bacillus pseudofirmus FA30–01. Extremophiles 10:229–235
Lin X, Inglis GD, Yanke LJ, Cheng KJ (1999) Selection and characterization of feather-degrading bacteria from canola meal compost. J Ind Microbiol Biotechnol 23:149–153
Matsui T, Yamada Y, Mitsuya H, Shigeri Y, Yoshida Y, Saito Y, Matsui H, Watanabe K (2009) Sustainable and practical degradation of intact chicken feathers by cultivating a newly isolated thermophilic Meiothermus ruber H328. Appl Microbiol Biotechnol 82(5):941–950
Nadir RM, Cleison LT, Daniela CV, Terezinha IES, Marina KK, Rosane MP (2008) New feather-degrading filamentous fungi. Microb Ecol 56:13–17
Onifade AA, Al-Sane NA, Al Musallam AA, Al-Zarban S (1998) A review: potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Bioresour Technol 66:1–11
Stanford M (1968) Amino acid analysis: aqueous dimethyl sulfoxide as solvent for the ninhydrin reaction. J Biol Chem 243(23):6281–6283
Tang QY, Feng MG (2007) DPS data processing system: experimental design, statistical analysis, and data mining. Science Press, Beijing
Tu GQ, Ding J (1994) A primary identification of a feather-degrading Streptomyces fradiae Var S-221 strain. J Jiangxi Agric Univ 16(04):399–403
Worapot S, Junthip T, Poranee O, Hiroshi O, Kanok R, Thanit K, Kohei O (2005) Purification and characterisation of keratinase from a thermotolerant feather-degrading bacterium. World J Microbiol Biotechnol 21:1111–1117
Wu QL, Chen T, Gan Y, Chen X, Zhao XM (2007) Optimization of riboflavin production by recombinant Bacillus subtilis RH44 using statistical designs. Appl Microbiol Biotechnol 76:783–794
Zhuang YP, Chen B, Chu J, Zhang SL (2006) Medium optimization for meilingmycin production by Streptomyces nanchangensis using response surface methodology. Process Biochem 41:405–409
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Cheng, X., Huang, L., Tu, Xr. et al. Medium optimization for the feather-degradation by Streptomyces fradiae Var S-221 using the response surface methodology. Biodegradation 21, 117–122 (2010). https://doi.org/10.1007/s10532-009-9286-7
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DOI: https://doi.org/10.1007/s10532-009-9286-7