Microbial populations related to PAH biodegradation in an aged biostimulated creosote-contaminated soil
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A previous bioremediation survey on a creosote-contaminated soil showed that aeration and optimal humidity promoted depletion of three-ringed polycyclic aromatic hydrocarbons (PAHs), but residual concentrations of four-ringed benzo(a)anthracene (B(a)A) and chrysene (Chry) remained. In order to explain the lack of further degradation of heavier PAHs such as four-ringed PAHs and to analyze the microbial population responsible for PAH biodegradation, a chemical and microbial molecular approach was used. Using a slurry incubation strategy, soil in liquid mineral medium with and without additional B(a)A and Chry was found to contain a powerful PAH-degrading microbial community that eliminated 89% and 53% of the added B(a)A and Chry, respectively. It is hypothesized that the lack of PAH bioavailability hampered their further biodegradation in the unspiked soil. According to the results of the culture-dependent and independent techniques Mycobacterium parmense, Pseudomonas mexicana, and Sphingobacterials group could control B(a)A and Chry degradation in combination with several microorganisms with secondary metabolic activity.
KeywordsPolycyclic aromatic hydrocarbons Biodegradation Bioavailability Bioremediation Pyrene Chrysene 16SrRNA DGGE
This study was financially supported by the Spanish Ministry of Science and Technology (CTM2007-61097/TECNO) and by the Reference Network in Biotechnology (XERBA) of the Autonomous Government of Catalonia. The authors declare that the experiments discussed in this paper were performed in compliance with current Spanish and European legislation.
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