Identification of novel denitrifying bacteria Stenotrophomonas sp. ZZ15 and Oceanimonas sp. YC13 and application for removal of nitrate from industrial wastewater
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Two novel denitrifying bacteria were successfully isolated from industrial wastewater and soil samples. Using morphological, biochemical/biophysical and 16S rRNA gene analyses, these two bacteria were identified as Stenotrophomonas sp. ZZ15 and Oceanimonas sp. YC13, respectively. Both of these two bacteria showed efficient NO3 −-N removing abilities under a semi-anaerobic condition without obvious accumulation of NO2 −-N, N2O-N and NH4 +-N. NO3 −-N removal from paper mill wastewater was also successful by treatments with either a denitrifier or an immobilization method. Therefore, this study provides valuable denitrifying bacteria in biotreatment of industrial wastewater and other environmental pollution caused by NO3 −/NO2 −.
KeywordsBiological nitrate removal Stenotrophomonas Oceanimonas Denitrifying bacteria Industrial wastewater Immobilization
LY and YL were supported by the Student Research Fund (SRF) of Huazhong Agricultural University. This work was funded by the National Natural Science Foundation, P. R. China (30671140), and Microbial Resources Project of the Ministry of Science and Technology, P. R. China (2005DKA21208-6). The authors would like to express their gratitude to Drs. Christopher Rensing, Junchu Zhou and Yunxiang Liang for their scientific comments and technical supports.
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