, Volume 18, Issue 5, pp 625–636 | Cite as

Benzene oxidation under sulfate-reducing conditions in columns simulating in situ conditions

  • Carsten Vogt
  • Stefan Gödeke
  • Hanns-Christian Treutler
  • Holger Weiß
  • Mario Schirmer
  • Hans-Hermann Richnow
Original Paper


The oxidation of benzene under sulfate-reducing conditions was examined in column and batch experiments under close to in situ conditions. Mass balances and degradation rates for benzene oxidation were determined in four sand and four lava granules filled columns percolated with groundwater from an anoxic benzene-contaminated aquifer. The stoichiometry of oxidized benzene, produced hydrogen carbonate and reduced sulfate correlated well with the theoretical equation for mineralization of benzene with sulfate as electron acceptor. Mean retention times of water in four columns were determined using radon (222Rn) as tracer. The retention times were used to calculate average benzene oxidation rates of 8–36 μM benzene day−1. Benzene-degrading, sulfide-producing microcosms were successfully established from sand material of all sand filled columns, strongly indicating that the columns were colonized by anoxic benzene-degrading microorganisms. In general, these data indicate a high potential for Natural Attenuation of benzene under sulfate-reducing conditions at the field site Zeitz. In spite of this existing potential to degrade benzene with sulfate as electron acceptor, the benzene plume at the field site is much longer than expected if benzene would be degraded at the rates observed in the column experiment, indicating that benzene oxidation under sulfate-reducing conditions is limited in situ.


Benzene Degradation Natural attenuation Sulfate-reducing conditions 



This work is integrated in the internal research and development program of the UFZ as well as the SAFIRA I project. The authors thank Werner Kletzander, Ralf Trabitzsch and Jörg Ahlheim of the Department of Groundwater Remediation for column sampling and organizational help, and Dietmar Hähnel for analyzing BTEX, sulfide, sulfate and carbonate during the column experiment. Special thanks is addressed to Stephanie Hinke for preparing culture media and analyzing samples of sulfide and benzene during the microcosm experiment.


  1. Aksoy M (1985) Benzene as a leukemogenic and carcinogenic agent. Am J Ind Med 8:9–20CrossRefGoogle Scholar
  2. Alfreider A, Krossbacher M, Psenner R (1997) Groundwater samples do not reflect bacterial densities and activity in subsurface systems. Water Res 31:832–840CrossRefGoogle Scholar
  3. Anderson RT, Lovley DR (2000) Anaerobic bioremediation of benzene under sulfate reducing conditions in a petroleum-contaminated aquifer. Environ Sci Technol 34:2261–2266CrossRefGoogle Scholar
  4. Aronson D, Howard PH (1997) Anaerobic degradation of organic chemicals in groundwater: a summary of field and laboratory studies. Final Report. Environmental Science Center, Syracus Research Cooperation, New York, pp. 244Google Scholar
  5. Atlas RM (1981) Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiol Rev 45:180–209Google Scholar
  6. Burland SM, Edwards EA (1999) Benzene biodegradation linked to nitrate reduction. Appl Environ Microbiol 65:529–533Google Scholar
  7. Caldwell ME, Suflita JM (2000) Detection of phenol and benzoate as intermediates of anaerobic benzene biodegradation under different terminal electron-accepting conditions. Environ Sci Technol 34:1216–1220CrossRefGoogle Scholar
  8. Chakraborty R, Coates JD (2004) Anaerobic degradation of monoaromatic hydrocarbons. Appl Microbiol Biotechnol 64:437–446CrossRefGoogle Scholar
  9. Chakraborty R, Coates JD (2005) Hydroxylation and carboxylation—two crucial steps of anaerobic benzene degradation by Dechloromonas strain RCB. Appl Environ Microbiol 71:5427–5432CrossRefGoogle Scholar
  10. Coates JD, Chakraborty R, Lack JG, O’Connor SM, Cole KA, Bender KS, Achenbach LA (2001) Anaerobic benzene oxidation coupled to nitrate reduction in pure culture by two strains of Dechloromonas. Nature 411:1039–1043CrossRefGoogle Scholar
  11. Cunningham JA, Rahme H, Hopkins GD, Lebron C, Reinhard M (2001) Enhanced in situ bioremediation of BTEX-contaminated groundwater by combined injection of nitrate and sulphate. Environ Sci Technol 35:1663–1670CrossRefGoogle Scholar
  12. Da Silva MLB, Alvarez PJJ (2004) Enhanced anaerobic biodegradation of benzene–toluene–ethylbenzene–xylene–ethanol mixtures in bioaugmented aquifer columns. Appl Environ Microbiol 70:4720–4726CrossRefGoogle Scholar
  13. Dean BJ (1985) Recent findings on the genetic toxicology of benzene, toluene, xylenes and phenols. Mutat Res 145:153–181Google Scholar
  14. Edwards EA, Grbic-Galic D (1992) Complete mineralization of benzene by aquifer microorganisms under strictly anaerobic conditions. Appl Environ Microbiol 58:2663–2666Google Scholar
  15. Freyer K, Treutler HC, Just G, von Philipsborn H (2003) Optimization of time resolution and detection limit for online measurements of 222Rn in water. J Radioanal Nucl Chem 257:129–132CrossRefGoogle Scholar
  16. Gödeke S, Richnow HH, Weiß H, Fischer A, Vogt C, Borsdorf H, Schirmer M (2006) Multi component-reactive tracer test for the implementation of enhanced in-situ bioremediation at a BTEX-contaminated megasite. J Contam Hydrol 87:211–236CrossRefGoogle Scholar
  17. Grbic-Galic D, Vogel TM (1987) Transformation of toluene and benzene by mixed methanogenic cultures. Appl Environ Microbiol 53:254–260Google Scholar
  18. Griebler C, Mindl B, Slezak D, Geiger-Kaiser M (2002) Distribution pattern of attached and suspended bacteria in pristine and contaminated shallow aquifers studies with an in situ sediment exposure. Aquat Microb Ecol 28:117–129Google Scholar
  19. Hazen TC, Jimenez L, Lopez de Victoria G, Fliermans CB (1991) Comparison of bacteria from deep subsurface sediments and adjacent groundwater. Microb Ecol 22:293–304CrossRefGoogle Scholar
  20. Johnson SJ, Woolhouse KJ, Prommer H, Barry DA, Christofi N (2003) Contribution of anaerobic microbial activity to natural attenuation of benzene in groundwater. Eng Geol 70:343–349CrossRefGoogle Scholar
  21. Kazumi J, Caldwell ME, Suflita JM, Lovley DR, Young LY (1997) Anaerobic degradation of benzene in diverse anoxic environments. Environ Sci Technol 31:813–818CrossRefGoogle Scholar
  22. Kölbel-Boelke J, Anders EM, Nehrkorn A (1988) Microbial communities in the saturated groundwater environment. II: diversity of bacterial communities in a pleistocene sand aquifer and their in vitro activities. Microb Ecol 16:31–48CrossRefGoogle Scholar
  23. Lovley DR, Coates JD, Woodward JC, Phillips EJP (1995) Benzene oxidation coupled to sulfate reduction. Appl Environ Microbiol 61:953–958Google Scholar
  24. Nales M, Butler BJ, Edwards EA (1998) Anaerobic benzene biodegradation: a microcosm survey. Bioremediation J 2:125–144CrossRefGoogle Scholar
  25. Phelps CD, Young LY (1999) Anaerobic biodegradation of BTEX and gasoline in various aquatic sediments. Biodegradation 10:15–25CrossRefGoogle Scholar
  26. Ruiz-Aguilar GML, O’Reilly K, Alvarez PJJ (2003) A comparison of benzene and toluene plume lengths for sites contaminated with regular vs. ethanol-amended gasoline. Ground Water Monit Remediation 23:48–53CrossRefGoogle Scholar
  27. Schirmer M, Dahmke A, Dietrich P, Dietze M, Gödeke S, Richnow HH, Schirmer K, Weiß H, Teutsch G (2006) Natural attenuation research at the contaminated megasite Zeitz. J Hydrol 328:393–407CrossRefGoogle Scholar
  28. Thornton SF, Quigley S, Spence MJ, Banwart SA, Bottrell S, Lerner DN (2001) Processes controlling the distribution and natural attenuation of dissolved phenolic compounds in a deep sandstone aquifer. J Contam Hydrol 53:233–267CrossRefGoogle Scholar
  29. Thullner M, Mauclaire L, Schroth MH, Kinzelbach W, Zeyer J (2002) Interaction between water flow and spatial distribution of microbial growth in a two-dimensional flow field in saturated porous media. J Contam Hydrol 58:169–189CrossRefGoogle Scholar
  30. Ulrich AC, Beller HR, Edwards EA (2005) Metabolites detected during biodegradation of 13C6-benzene in nitrate-reducing and methanogenic enrichment cultures. Environ Sci Technol 39:6681–6691CrossRefGoogle Scholar
  31. Van Agteren MH, Keuning S, Janssen DB (1998) Handbook on biodegradation and biological treatment of hazardous organic compounds. Kluwer Academic Publishers, DordrechtGoogle Scholar
  32. Vieth A, Kästner M, Schirmer M, Weiß H, Gödeke S, Meckenstock RU, Richnow HH (2005) Monitoring in situ biodegradation of benzene and toluene by stable carbon isotope fractionation. Environ Toxicol Chem 24:51–60CrossRefGoogle Scholar
  33. Weiner JM, Lovley DR (1998) Anaerobic benzene degradation in petroleum-contaminated aquifer sediments after inoculation with a benzene-oxidizing enrichment. Appl Environ Microbiol 64:775–778Google Scholar
  34. Weiner JM, Lauck TS, Lovley DR (1998) Enhanced anaerobic benzene degradation with the addition of sulfate. Bioremediation J 2:159–173CrossRefGoogle Scholar
  35. Widdel F, Rabus R (2001) Anaerobic biodegradation of saturated and aromatic hydrocarbons. Curr Opin Biotechnol 12:259–276CrossRefGoogle Scholar
  36. Wiedemeier TH, Newell CJ, Rifai HS, Wilson JT (1999) Natural attenuation of fuels and chlorinated solvents in the subsurface. Wiley, New YorkGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Carsten Vogt
    • 1
  • Stefan Gödeke
    • 2
  • Hanns-Christian Treutler
    • 3
  • Holger Weiß
    • 2
  • Mario Schirmer
    • 4
  • Hans-Hermann Richnow
    • 1
  1. 1.Department of Isotope BiogeochemistryHelmholtz Centre for Environmental Research - UFZLeipzigGermany
  2. 2.Department of Groundwater RemediationHelmholtz Centre for Environmental Research - UFZLeipzigGermany
  3. 3.Department of Analytical ChemistryHelmholtz Centre for Environmental Research - UFZLeipzigGermany
  4. 4.Department of HydrogeologyHelmholtz Centre for Environmental Research - UFZLeipzigGermany

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