Mass production of bacterial communities adapted to the degradation of volatile organic compounds (TEX)
- 98 Downloads
This study focuses on the mass cultivation of bacteria adapted to the degradation of a mixture composed of toluene, ethylbenzene, o-, m- and p-xylenes (TEX). For the cultivation process Substrate Pulse Batch (SPB) technique was adapted under well-automated conditions. The key parameters to be monitored were handled by LabVIEW software including, temperature, pH, dissolved oxygen and turbidity. Other parameters, such as biomass, ammonium or residual substrate concentrations needed offline measurements. SPB technique has been successfully tested experimentally on TEX. The overall behavior of the mixed bacterial population was observed and discussed along the cultivation process. Carbon and nitrogen limitations were shown to affect the integrity of the bacterial cells as well as their production of exopolymeric substances (EPS). Average productivity and yield values successfully reached the industrial specifications, which were 0.45 kgDW m−3 d−1 and 0.59 gDW g C −1 , respectively. Accuracy and reproducibility of the obtained results present the controlled SPB process as a feasible technique.
KeywordsAutomated process Biodegradation BTEX Kinetics Metabolic adaptation Stripping
Unable to display preview. Download preview PDF.
The authors wish to thank the European Commission for financial support under the EUREKA project, “Bioreactor For Innovative Mass Bacteria Culture, BIOMAC” (see: www.eureka.be, project E!2497), as well as the CADOX Project (contract no. EVK1-CT-2002–00122).
- Acuna-Askar K, Englande AJ Jr., Ramirez-Medrano A, Coronado-Guardiola JE, Chavez-Gomez B (2003) Evaluation of biomass production in unleaded gasoline and BTEX-fed batch reactors. Wat Sci Technol 48(8):127–133Google Scholar
- De Bont J (1998) Solvent-tolerant bacteria in biocatalysis. Tibtech 16:493–499Google Scholar
- Duenas M, Mundate A, Perea A, Irastorza A (2003) Exopolysaccharides production by Pedioceccus damnosus 2.6 in a semi defined medium under different growth conditions. Int J Food Microbiol 2709:1–8Google Scholar
- Durnaz B, Sanin F (2001) Effects of carbon nitrogen ratio on the composition of microbial extracellular polymers in activated sludge. Water Sci Technol 44(1):221–229Google Scholar
- Lapertot M, Domeniconi P, Seignez C, Ebrahimi S, Peringer P (2005) Upgraded conventional cultivation techniques for the mass production of bacterial communities adapted to the degradation of Volatile Organic Compounds (TEX). Congress on Biotechniques for Air Pollution Control. La Coruña, Spain. In pressGoogle Scholar
- Sikkema J, De Bont J, Poolman B (1995) Mechanisms of solvent toxicity of hydrocarbons. Microbiol Rev 269:8022–8026Google Scholar
- Villatoro-Monzón WR, Mesta-Howard AM, Razo-Flores E (2003) Anaerobic biodegradation of BTEX using Mn(IV) and Fe(III) as alternative electron acceptors. Water Sci Technol 48(6):125–131Google Scholar