Abstract
Air artificially contaminated with increasing concentrations of benzene was treated in a laboratory scale compost-packed biofilter for 240 days with a removal efficiency of 81–100%. The bacterial community in the packing material (PM) at different heights of the biofilter was analysed every 60 days. Bacterial plate counts and ribosomal intergenic spacer analysis (RISA) of the isolated strains showed that the number of cultivable aerobic heterotrophic bacteria and the species diversity increased with benzene availability. Identification of the isolated species and the main bands in denaturing gradient gel electrophoresis (DGGE) profiles from total compost DNA during the treatment revealed that, at a relatively low volumetric benzene load (1.2≤VBL≤6.4 g m−3 PM h−1), besides low G+C Gram positive bacteria, originally present in the packing compost, bacteroidetes and β- and γ-proteobacteria became detectable in the colonising population. At the VBL value (24.8 g m−3 PM h−1) ensuring the maximum elimination capacity of the biofilter (20.1 g m−3 PM h−1), strains affiliated to the genus Rhodococcus dominated the microflora, followed by β-proteobacteria comprising the genera Bordetella and Neisseria. Under these conditions, more than 35% of the isolated strains were able to grow on benzene as the sole carbon source. Comparison of DGGE and automated RISA profiles of the total community and isolated strains showed that a complex bacterial succession occurred in the reactor in response to the increasing concentrations of the pollutant and that cultivable bacteria played a major role in benzene degradation under the adopted conditions.
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ME Acuña, F Pérez, R Auria and S Revah, Microbiological and kinetic aspect of a biofilter for the removal of toluene from waste gases. Biotechnol. Bioeng. 63 (1999) 175-183
A Ahrens, A Lipski, S Klatte, HJ Busse, G Auling and K Altendorf, Polyphasic classification of proteobacteria isolated from biofilters. Syst. Appl. Microbiol. 20 (1997) 255-267
FM Ausubel, R Brent, RE Kingston, DD Moore, JG Seidman, JA Smith and K Struhl, Current Protocols of Molecular Biology. USA: John Wiley and Sons (1994).
L Brusetti, P Francia, C Bertolini, A Pagliuca, S Borin, C Sorlini, A Abruzzese, G Sacchi, C Viti, L Giovannetti, E Giuntini, M Bazzicalupo and D Daffonchio, Bacterial rhizosphere community of transgenic Bt176 maize and its non transgenic counterpart. Plant Soil 266 (2004) 11-21
L Cavalca, P Di Gennaro, M Colombo, V Andreoni, S Bernasconi, I Ronco and G Bestetti, Distribution of catabolic pathways in some hydrocarbon-degrading bacteria from a subsurface polluted soil. Res. Microbiol. 51 (2000) 877-887
A Cherif, L Brusetti, S Borin, A Rizzi, A Boudabous, H Khyami-Horani and D Daffonchio, Genetic relationship in the ‘Bacillus cereus group’ by rep-PCR fingerprinting and sequencing of a Bacillus anthracis-specific rep-PCR fragment. J. Appl. Microbiol. 94 (2003) 1108-1119
D Daffonchio, S Borin, A Consolandi, D Mora, P Manachini and C Sorlini, 16–23S rRNA internal transcribed spacers as molecular markers for the species of the 16S rRNA group I of the genus Bacillus. FEMS Microbiol. Lett. 163 (1998) 229-236
PM Dees and WC Ghiorse, Microbial diversity in hot synthetic compost as revealed by PCR-amplified rRNA sequences from cultivated isolates and extracted DNA. FEMS Microbiol. Ecol. 35 (2001) 207-216
U Friedrich, K Prior, K Altendorf and A Lipski, High bacterial diversity of a waste gas-degrading community in an industrial biofilter as shown by a 16S rDNA clone library. Environ. Microbiol. 4 (2002) 721-734
JR Hanson, CE Ackerman and KM Scow, Biodegradation of methyl tert-butyl ether by a bacterial pure culture. Appl. Environ. Microbiol. 65 (1999) 4788-4792
Heuer H & Smalla K (1997) Application of Denaturing Gel Electrophoresis and Temperature Gradient Gel Electropho- resis for studying soil microbial communities. In: Van Elsas JD, Trevors JT and Wellington EMH (Eds) Modern Soil Microbiology, (pp 353–370). New York.
P Juteau, R Larocque, D Rho and A LeDuy, Analysis of the relative abundance of different types of bacteria capable of toluene degradation in a compost biofilter. Appl. Microbiol. Biotechnol. 52 (1999) 863-868
N Lansac, FJ Picard, C Ménard, M Boissinot, M Ouellette, PH Roy and MG Bergeron, Novel genus-specific PCR-based assays for rapid identification of Neisseria species and Neisseria meningitidis. Eur. J. Clin. Microbiol. Infect. Dis. 19 (2000) 443-451
G Leson and AM Winer, Biofiltration: an innovative air pollution control technology for VOC emissions. J. Air Waste Manag. Assoc. 41 (1991) 1045-1054
A Lipski and K Altendorf, Identification of heterotrophic bacteria isolated from ammonia-supplied experimental biofilters. Syst. Appl. Microbiol. 20 (1997) 448-457
S Møller, AR Pedersen, LK Poulsen, E Arvin and S Molin, Activity and three-dimensional distribution of toluene-degrading Pseudomonas putida in a multispecies biofilm assessed by quantitative in situ hybridisation and scanning confocal laser microscopy. Appl. Environ. Microbiol. 62 (1996) 4632-4640
W Namkoong, JS Park and JS Gheynst Van der, Effect of gas velocity and influent concentration on biofiltration of gasoline off-gas from soil vapor extraction. Chemosphere 57 (2004) 721-730
S Peters, S Koschinsky, F Schwieger and CC Tebbe, Succession of microbial communities during hot composting as detected by PCR-single-stranded-conformation-polymorphism-based genetic profiles of small-subunit rRNA genes. Appl. Environ. Microbiol. 66 (2000) 930-936
S Roy, J Gendron, MC Delhoménie, L Bibeau, M Heitz and R Brezinki, Pseudomonas putida as the dominat toluene-degrading bacterial species during air decontamination by biofiltration. Appl. Microbiol. Biotechnol. 61 (2003) 366-373
Y Sakano and L Kerkhof, Assessment of changes in microbial community structure during operation of an ammonia biofilter with molecular tools. Appl. Environ. Microbiol. 64 (1998) 4877-4882
J Sambrook, EF Fritsch and T Maniatis, Molecular Cloning: A Laboratory Manual. 1,2,3,. NY: Cold Spring Harbor Laboratory Press (1989).
AM Sass, H Sass, MJL Coolen, H Cypionka and J Overmann, Microbial communities in the chemocline of a hypersaline deep-sea basin (Urania Basin, Mediterranean Sea). Appl. Environ. Microbiol. 67 (2001) 5392-5402
L Sene, A Converti, MG Felipe and M Zilli, Sugarcane bagasse as alternative packing material for biofiltration of benzene polluted gaseous streams: a preliminary study. Biores. Technol. 83 (2002) 153-157
C Urzì, L Brusetti, S Salamone, C Sorlini, E Stackebrandt and D Daffonchio, Frequency and biodiversity of Geodermatophilaceae isolated from altered stones in the Mediterranean basin. Environ. Microbiol. 3 (2001) 471-479
JD Hamme Van, A Singh and OP Ward, Recent advances in petroleum microbiology. Microbiol. Mol. Biol. Rev. 67 (2003) 503-549
MC Veiga and C Kennes, Parameters affecting performance and modelling of biofilters treating alkylbenzene-polluted air. Appl. Microbiol. Biotechnol. 55 (2001) 254-258
F Von Wintzingerode, A Schattke, RA Siddiqui, U Rösick, UB Göbel and R Gross, Bordetella petrii Nov., isolated from an anaerobic bioreactor, and emended description of the genus Bordetella. Int. J. Syst. Evol. Microbiol. 51 (2001) 1257-1265
M Zilli and A Converti, Biofilters. In: MC Flickinger and SW Drew (eds.) The Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation. New York: Wiley (1999) pp. 305-319
M Zilli, D Daffonchio, R Di Felice, M Giordani and A Converti, Treatment of benzene-contaminated airstreams in laboratory-scale biofilters packed with raw and sieved sugarcane bagasse and with peat. Biodegradation 15 (2004) 87-96
Zilli M, Guarino C, Daffonchio D, Borin S & Converti A (2005) Laboratory-scale experiments with a powdered compost biofilter treating benzene-polluted air. Proc. Biochem., in press, available online 29 September 2004.
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Borin, S., Marzorati, M., Brusetti, L. et al. Microbial Succession in a Compost-packed Biofilter Treating Benzene-contaminated Air. Biodegradation 17, 79–89 (2006). https://doi.org/10.1007/s10532-005-7565-5
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DOI: https://doi.org/10.1007/s10532-005-7565-5