Biological Invasions

, Volume 17, Issue 8, pp 2229–2234 | Cite as

Elevated, but highly variable, acetylene reduction in soils associated with the invasive shrub Rhamnus cathartica in a Midwestern oak forest

  • Patrick M. Ewing
  • Domokos Lauko
  • Mike Anderson
Invasion Note


Common buckthorn (Rhamnus cathartica) is an important invasive shrub in North American forests, where it is thought that the plant’s retention of green leaves during autumn canopy senescence helps it succeed in deciduous understory habitat. This trait results in loss of nitrogen (N) in N-rich leaf litter, which has led some workers to suggest buckthorn may foster associative N-fixation in soil. We examined this possibility in an oak woodland understory in eastern Minnesota using the acetylene reduction assay to compare apparent nitrogenase activity in soils collected from beneath buckthorn individuals with soils collected from a canopy species (Quercus spp.), an important understory shrub (Prunus serotina), and non-vegetated areas. Buckthorn and non-buckthorn soils differed in variability of acetylene reduction (AR) rates, with buckthorn values covering a range 10× the range of non-buckthorn soils. Mean AR also differed between buckthorn and non-buckthorn soils, but the direction and magnitude of the difference varied with sampling location. Estimates of N inputs calculated from our data suggest that AR values at the high end of the buckthorn-associated range are biologically significant. Our results represent the first measurement of AR activity associated with common buckthorn, and are consistent with the hypothesis that this plant supports associative N fixation under some conditions. Suggestions for follow-up studies are provided.


Rhamnus cathartica Acetylene reduction assay Frankia Nitrogen fixation Invasive species Invasion mechanism 


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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Patrick M. Ewing
    • 1
  • Domokos Lauko
    • 2
  • Mike Anderson
    • 3
  1. 1.Department of Agronomy and Plant GeneticsUniversity of MinnesotaSaint PaulUSA
  2. 2.Department of Plant and Microbial BiologyUniversity of California, BerkeleyBerkeleyUSA
  3. 3.Biology DepartmentMacalester CollegeSaint PaulUSA

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