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Biological Invasions

, Volume 17, Issue 8, pp 2229–2234 | Cite as

Elevated, but highly variable, acetylene reduction in soils associated with the invasive shrub Rhamnus cathartica in a Midwestern oak forest

  • Patrick M. Ewing
  • Domokos Lauko
  • Mike Anderson
Invasion Note

Abstract

Common buckthorn (Rhamnus cathartica) is an important invasive shrub in North American forests, where it is thought that the plant’s retention of green leaves during autumn canopy senescence helps it succeed in deciduous understory habitat. This trait results in loss of nitrogen (N) in N-rich leaf litter, which has led some workers to suggest buckthorn may foster associative N-fixation in soil. We examined this possibility in an oak woodland understory in eastern Minnesota using the acetylene reduction assay to compare apparent nitrogenase activity in soils collected from beneath buckthorn individuals with soils collected from a canopy species (Quercus spp.), an important understory shrub (Prunus serotina), and non-vegetated areas. Buckthorn and non-buckthorn soils differed in variability of acetylene reduction (AR) rates, with buckthorn values covering a range 10× the range of non-buckthorn soils. Mean AR also differed between buckthorn and non-buckthorn soils, but the direction and magnitude of the difference varied with sampling location. Estimates of N inputs calculated from our data suggest that AR values at the high end of the buckthorn-associated range are biologically significant. Our results represent the first measurement of AR activity associated with common buckthorn, and are consistent with the hypothesis that this plant supports associative N fixation under some conditions. Suggestions for follow-up studies are provided.

Keywords

Rhamnus cathartica Acetylene reduction assay Frankia Nitrogen fixation Invasive species Invasion mechanism 

References

  1. Anderson MD, Ruess RW, Uliassi DD, Mitchell JS (2004) Estimating N2 fixation in two species of Alnus in interior Alaska using acetylene reduction and 15N2 uptake. Ecoscience 11:102–112Google Scholar
  2. Boring LR, Swank WT, Waide JB, Henderson GS (1988) Sources, fates, and impacts of nitrogen inputs to terrestrial ecosystems: review and synthesis. Biogeochem 6:119–159CrossRefGoogle Scholar
  3. Burgess BK, Lowe DJ (1996) Mechanism of molybdenum nitrogenase. Chem Rev 96:2983–3011PubMedCrossRefGoogle Scholar
  4. Chapin FS III, Walker LR, Fastie CL, Sharman LC (1994) Mechanisms of primary succession following deglaciation at Glacier Bay, Alaska. Ecol Monogr 64:149–175CrossRefGoogle Scholar
  5. Hardy RWF, Holsten RD, Jackson EK, Burns RC (1968) The acetylene ethylene assay for N2 fixation: laboratory and field evaluation. Plant Phys 43:1185–1207CrossRefGoogle Scholar
  6. Harrington RA, Brown BJ, Reich PB (1989) Ecophysiology of exotic and native shrubs in southern Wisconsin I: relationship of leaf characteristics, resource availability, and phenology to seed patterns and carbon gain. Oecologia 80:356–367CrossRefGoogle Scholar
  7. Henegan L, Rauschenberg C, Fatemi F, Workman M (2004) European buckthorn (Rhamnus cathartica) and its effects on some ecosystem properties in an urban woodland. Ecol Restor 22:275–280CrossRefGoogle Scholar
  8. Henegan L, Fatemi F, Umek L, Grady K, Fagen K, Workman M (2006) The invasive shrub European buckthorn (Rhamnus cathartica, L.) alters soil properties in Midwestern US woodlands. Appl Soil Ecol 32:142–148CrossRefGoogle Scholar
  9. Henegan L, Steffen J, Fagen K (2007) Interactions of an introduced shrub and introduced earthworms in an Illinois urban woodland: impact on leaf litter decomposition. Pedobiologia 50:543–551CrossRefGoogle Scholar
  10. Knight KS, Kurylo JS, Endress AG, Stewart JR, Reich PB (2007) Ecology and ecosystem impacts of common buckthorn (Rhamnus cathartica): a review. Biol Invasions 9:925–937CrossRefGoogle Scholar
  11. Langkamp PJ, Farnell GK, Dalling MJ (1982) Nutrient cycling in a stand of Acacia holosericea A. Cunn. Ex G. Don. I. Measurements of precipitation, interception, seasonal acetylene reduction, plant growth and nitrogen requirement. Aust J Bot 30:87–106CrossRefGoogle Scholar
  12. Madritch MD, Lindroth RL (2009) Removal of invasive shrubs reduces exotic earthworm populations. Biol Invasions 11:663–671CrossRefGoogle Scholar
  13. Nohrstedt HÖ (1983) Natural formation of ethylene in forest soils and methods to correct results given by the acetylene-reduction assay. Soil Biol Biochem 15:281–286CrossRefGoogle Scholar
  14. Nohrstedt HÖ (1985) Nonsymbiotic nitrogen fixation in the topsoil of some forest stands in central Sweden. Can J For Res 15:715–722CrossRefGoogle Scholar
  15. Nohrstedt HÖ (1988) Nitrogen fixation (C2H2-reduction) in birch litter. Scand J For Res 3:17–23CrossRefGoogle Scholar
  16. Reed SC, Cleveland CC, Townsend AR (2011) Functional ecology of free-living nitrogen fixation: a contemporary perspective. Ann Rev Ecol Evol Syst 42:489–512CrossRefGoogle Scholar
  17. Smolander A (1990) Frankia populations in soils under different tree species—with special emphasis on soils under Betula pendula. Plant Soil 121:1–10CrossRefGoogle Scholar
  18. Son Y (2001) Non-symbiotic nitrogen fixation in forest ecosystems. Ecol Res 16:183–196CrossRefGoogle Scholar
  19. Stewart JR, Kennedy GJ, Reid RD, Dawson JO (2008) Foliar-nitrogen and phosphorous resorption patterns differ among nitrogen-fixing and nonfixing temperate-deciduous trees and shrubs. Int J Plant Sci 169:495–502CrossRefGoogle Scholar
  20. Stutz and Bliss (1973) Acetylene reduction assay for nitrogen fixation under field conditions in remote areas. Plant Soil 38:209–213CrossRefGoogle Scholar
  21. Tateno M (2003) Benefit to N2-fixing alder of extending growth period at the cost of leaf nitrogen loss without resorption. Oecologia 137:338–343PubMedCrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Patrick M. Ewing
    • 1
  • Domokos Lauko
    • 2
  • Mike Anderson
    • 3
  1. 1.Department of Agronomy and Plant GeneticsUniversity of MinnesotaSaint PaulUSA
  2. 2.Department of Plant and Microbial BiologyUniversity of California, BerkeleyBerkeleyUSA
  3. 3.Biology DepartmentMacalester CollegeSaint PaulUSA

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