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Biological Invasions

, Volume 11, Issue 2, pp 225–239 | Cite as

Diversity and functional responses of nitrogen-fixing microbes to three wetland invasions

  • Serena M. Moseman
  • Rui Zhang
  • Pei Yuan Qian
  • Lisa A. Levin
Original Paper

Abstract

Impacts of invasive species on microbial components of wetland ecosystems can reveal insights regarding functional consequences of biological invasions. Nitrogen fixation (acetylene reduction) rates and diversity of nitrogen fixers, determined by genetic fingerprinting (T-RFLP) of the nifH gene, were compared between native and invaded sediments in three systems. Variable responses of nitrogen fixing microbes to invasion by a non-native mussel, Musculista senhousia, and mangrove, Avicennia marina, in Kendall Frost-Northern Wildlife Preserve (Mission Bay) and salt cedar, Tamarisk (Tamarix spp.) in Tijuana Estuary suggest microbes respond to both species- and site-specific influences. Structurally similar invaders (the mangrove and salt cedar) produced different effects on activity and diversity of nitrogen fixers, reflecting distinct environmental contexts. Despite relative robustness of microbial community composition, subtle differences in total diversity or activity of nitrogen fixers reveal that microbes are not immune to impacts of biological invasions, and that functional redundancy of microbial diversity is limited, with significant consequences for functional dynamics of wetlands.

Keywords

Asian mussel Diazotroph Mangrove Salt cedar Functional redundancy 

Notes

Acknowledgements

The authors thank Christine R. Whitcraft, Jeffrey A. Crooks, and Amanda J. Demopolous for providing environmental data. Generous support from Pei-Yuan Qian of the Hong Kong University of Science and Technology enabled T-RFLP applications and collaborations. Funding for this research was provided by the National Science Foundation, the National Estuarine Research Reserve System (Graduate Research Fellowships for Serena Moseman), and the University of California Natural Reserve System (Mildred Mathias Student Research Grant). The Michael M. Mullin and Mia Tegner Memorial Funds and the Graduate Department of Scripps Institution of Oceanography also provided financial support. Helpful comments and guidance were provided by Carolyn Currin, and James Leichter. Facilities and gas chromatography equipment were provided by Lihini Aluwihare. Equipment for DNA extractions were provided by the Center for Marine Biodiversity and Conservation at Scripps Institution of Oceanography. Guillermo Mendoza provided guidance with Primer software operation and applications. Pat McMillan gave valuable formatting assistance. Tracy Washington and Maria del Carmen Rivero assisted with laboratory analyses.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Serena M. Moseman
    • 1
  • Rui Zhang
    • 2
    • 3
  • Pei Yuan Qian
    • 3
  • Lisa A. Levin
    • 1
  1. 1.Scripps Institution of OceanographyLa JollaUSA
  2. 2.National University of SingaporeSingaporeSingapore
  3. 3.Coastal Marine LaboratoryHong Kong University of Science and TechnologyKowloonHong Kong

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