The extraction of the hemicellulose fraction of sugarcane bagasse (SCB) by acid hydrolysis was evaluated in an autoclave and a Parr reactor aiming the application of the hydrolysate as a carbon source for lipid production by Lipomyces starkeyi.
The hydrolysis that resulted in the highest sugar concentration was obtained by treatment in the Parr reactor (HHR) at 1.5% (m/v) H2SO4 and 120 °C for 20 min, reaching a hemicellulose conversion of approximately 82%. The adaptation of the yeast to the hydrolysate provided good fermentability and no lag phase. The fermentation of hemicellulose-derived sugars (HHR) by L. starkeyi resulted in a 27.8% (w/w) lipid content and YP/S of 0.16 g/l.h. Increasing the inoculum size increased the lipid content by approximately 61%, reaching 44.8% (w/w).
The hemicellulose hydrolysate from SCB is a potential substrate for L. starkeyi to produce lipids for biodiesel synthesis based on the biorefinery concept.
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Aguilar R, Ramirez JA, Garrote G, Vázquez M (2002) Kinetic study of the acid hydrolysis of sugar cane bagasse. J Food Eng 55:309–318
Almeida JRM, Modig T, Petersson A, Hähn-Hägerdal B, Lidén G, Gorwa-Grauslund MF (2007) Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae. J Chem Technol Biot 4(82):340–349
Anschau A, Xavier MCA, Hernalsteens S, Franco TT (2014) Effect of feeding strategies on lipid production by Lipomyces starkeyi. Bioresour Technol 157:214–222
Aristizabal RVS (2013) Produção de leveduras oleaginosas em meio de cultura contendo hidrolisado de bagaço de cana-de-açúcar. Dissertation, State University of Campinas.
Balan V (2014) Current challenges in commercially producing biofuels from lignocellulosic biomass. ISRN Biotechnol. https://doi.org/10.1155/2014/463074
Bharathiraja B, Sridharan S, Sowmya V, Yuvaraj D, Praveenkumar R (2017) Microbial Oil - A Plausible Alternate Resource for Food and Fuel Application. Bioresour Technol 233:423–432. https://doi.org/10.1016/j.biortech.2017.03.006
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Bonturi N, Matsakas L, Nilsson R, Christakopoulos P, Miranda E, Berglund K, Rova U (2015) Single Cell Oil Producing Yeasts Lipomyces starkeyi and Rhodosporidium toruloides: Selection of Extraction Strategies and Biodiesel Property Prediction. Energies 8(6):5040–5052
Bonturi N, Crucello A, Viana AJC, Miranda EA (2017) Microbial oil production in sugarcane bagasse hemicellulosic hydrolysate without nutrient supplementation by a Rhodosporidium toruloides adapted strain. Process Biochem 57:16–25
Brandenburg J, Blomqbist J, Pickova J, Bonturi N, Sandgren M, Passoth V (2016) Lipid production from hemicellulose with Lipomyces starkeyi in a pH regulated fed-batch cultivation. Yeast 33:451–462
Canilha L, Carvalho W, Felipe M, Silva JB, Giulietti M (2010) Ethanol production from sugarcane bagasse hydrolysate using Pichia stipitis. Appl Biochem Biotechnol 161:84–92
Canilha L, Santos VT, Rocha GJ, Silva JB, Giulietti M, Silva SS, Felipe MG, Ferraz A, Milagres AM, Carvalho W (2011) A study on the pretreatment of a sugarcane bagasse sample with dilute sulfuric acid. J Ind Microbiol Biotechnol 38(9):1467–1475
Canilha L, Rodrigues RDCLB, Antunes FAF, Chandel AK, Milessi TSDS, Felipe MDGA, Silva SSD (2013) Bioconversion of Hemicellulose from Sugarcane Biomass Into Sustainable Products. In: Ekinci D (ed) Biochemistry, Genetics and Molecular Biology. InTech, London, pp 15–44
Chandel AK, Kapoor RK, Singh A, Kuhad RC (2007) Detoxification of sugarcane bagasse hydrolysate improves ethanol production by Candida shehatae NCIM 3501. Bioresour Technol 98(10):1947–1950
Chandel AK, Singh OV, Rao LV (2010) Biotechnological Applications of Hemicellulosic derived sugars:state-of-the-art. Sustain Biotechnol. https://doi.org/10.1007/978-90-481-3295-9_4
Chandel AK, Silva SS, Singh OV (2011) Detoxification of Lignocellulosic Hydrolysates for improved bioethanol production. InTech, London, pp 225–246
Christophe G, Kumar V, Nouaille R, Gaudet G, Fontanille P, Pandey A, Soccol CR, Larroche C (2012) Recent Developments in Microbial Oils Production: a Possible Alternative to Vegetable Oils for Biodiesel Without Competition with Human Food? Braz Arch Biol Technol 55(1):29–46
Dien BS, Slininger PJ, Kurtzman CP, Moser BR, O’bryan PJ, (2016) Identification of superior lipid producing Lipomyces and Myxozyma yeasts. AIMS Environ Sci 3(1):1–20
Feofilova EP, Sergeeva YE, Ivashechkin AA (2010) Biodiesel-fuel: Content, production, producers, contemporary biotechnology (Review). Appl Biochem Microbiol 46(4):369–378
Furlan FF, Filho RT, Pinto FHPB, Costa CBB, Cruz AJG, Giordano RLC, Giordano RC (2013) Bioelectricity versus bioethanol from sugarcane bagasse. Biotechnol Biofuels 6:142
Galán B, Santos-Merino M, Nogales J, de la Cruz F, García JL (2019) Microbial Oils as Nutraceuticals and Animal Feeds. In: Goldfine H (ed) Health Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids. Handbook of Hydrocarbon and Lipid Microbiology. Springer, Cham
Galbe M, Wallberg O (2019) Pretreatment for biorefneries: a review of common methods for eficient utilisation of lignocellulosic materials. Biotechnol Biofuels 12:294
Gao Q, Cui Z, Zhang J, Bao J (2014) Lipid fermentation of corncob residues hydrolysate by oleaginous yeast Trichosporon cutaneum. Bioresour Technol 152:552–556
Gao R, Li Z, Zhou X, Bao W, Cheng S, Zheng L (2020) Enhanced lipid production by Yarrowia lipolytica cultured with synthetic and waste-derived high-content volatile fatty acids under alkaline conditions. Biotechnol Biofuels 13:3
Garzón, C SL (2009) Produção microbiana de lipídios. Dissertation, State University of Campinas.
Gong Z, Wang Q, Shen H, Hu C, Jin G, Zhao ZK (2012) Co-fermentation of cellobiose and xylose by Lipomyces starkeyi for lipid production. Bioresour Technol 117:20–24
Gong Z, Shen H, Zhou W, Wang Y, Yang X, Zhao ZK (2015) Efficient conversion of acetate into lipids by the oleaginous yeast Cryptococcus curvatus. Biotechnol Biofuels 8:189
Gouveia ERN, Souto-Maior AM (2009) Validação de metodologia para a caracterização química de bagaço de cana-de-açúcar. Quim Nova 32(6):1500–1503
Hacisalihoğlu B, Turanli-Yildiz B, Çakar ZP (2018) Evolutionary Engineering Applications in Microbial Ethanol Production. JSM Biotechnol Biomed Eng 5(1):1082
Helmberger S, Kahr H, Jäger AG (2011) Yeast adaptation on the substrate straw. Bioenergy Technology (BE), in: World Renewable Energy Congress, Linkoping, Sweden 492–499. https://www.researchgate.net/publication/269131598_Yeast_Adaptation_on_the_Substrate_Straw Acessed January 2016.
Huang XF, Liu JN, Lu LJ, Peng KM, Yang GX, Liu J (2016) Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides. Bioresour Technol 206:141–149
Juanssilfero AB, Kahar P, Amza RL, Miyamoto N, Otsuka H, Matsumoto H, Kihira C, Thontowi A, Yopi OC, Prasetya B, Kondo A (2018) Effect of inoculum size on single-cell oil production from glucose and xylose using oleaginous yeast Lipomyces starkeyi. J Biosci Bioeng 125:695–702
Juanssilfero AB, Kahar P, Amza RL, Yopi SK, Ogino C, Prasetya B, Kondo A (2019) Lipid production by Lipomyces starkeyi using sap squeezed from felled old oil palm Trunks. J Biosci Bioeng 127(6):726–731
Kootstra AMJ, Beeftink HH, Scott EL, Sanders JPM (2009) Comparison of dilute mineral and organic acid pretreatment for enzymatic hydrolysis of wheat straw. Biochem Eng J 46(2):126–131
Koppram R, Albers E, Olsson L (2012) Evolutionary engineering strategies to enhance tolerance of xylose utilizing recombinant yeast to inhibitors derived from spruce biomass. Biotechnol Biofuels 5:32
Lenihan P, Orozco A, O’neill E, Ahmad MNM, Rooney DW, Walker GM (2010) Dilute acid hydrolysis of lignocellulosic biomass. Chem Eng J 156(2):395–403
Li Q, Metthew Lam LK, Xun L (2011) Biochemical characterization of ethanol-dependent reduction of furfural by alcohol dehydrogenases. Biodegradation 22(6):1227–1237
Liu JX, Yue QY, Gao BY, Ma ZH, Zhang PD (2012) Microbial treatment of the monosodium glutamate wastewater by Lipomyces starkeyi to produce microbial lipid. Bioresour Technol 106:69–73
Liu Y, Wang Y, Liu H, Zhang JA (2015a) Enhanced lipid production with undetoxified corncob hydrolysate by Rhodotorula glutinis using a high cell density culture strategy. Bioresour Technol 180:32–39
Liu ZJ, Liu LP, Wen P, Li N, Zong MH, Wu H (2015b) Effects of acetic acid and pH on growth and lipid accumulation of the oleaginous yeast Trichosporon fermentans. BioResources 10:4152–4166
Liu L, Zong M, Hu Y, Li N, Lou W, Wu H (2017) Efficient microbial oil production on crude glycerol by Lipomyces starkeyi AS 2.1560 and its kinetics. Process Biochem 58:230–238
Lopes HJS, Bonturi N, Miranda EA (2020) Rhodotorula toruloides Single Cell Oil Production Using Eucalyptus urograndis Hemicellulose Hydrolysate as a Carbon Source. Energies 13:795
Ma Y, Gao Z, Wang Q, Liu Y (2018) Biodiesels from microbial oils: Opportunity and challenges. Bioresour Technol 263:631–641
Manirakiza P, Covaci A, Schepens P (2001) Comparative study on total lipid determination using Soxhlet, Roese-Gottlieb, Bligh & Dyer and modified Bligh & Dyer extraction methods. J Food Compos Anal 100(14):93
Masri MA, Garbe D, Mehlmer N, Brück TB (2019) A sustainable, high-performance process for the economic production of waste-free microbial oils that can replace plant-based equivalents. Energy Environ Sci. https://doi.org/10.1039/C9EE00210C
Matsakas L, Sterioti AA, Rova U, Christakopoulos P (2014) Use of dried sweet sorghum for the efficient production of lipids by yeast Lipomyces starkeyi CBS 1807. Ind Crops Prod 62:367–372
Maza DD, Vinartab SC, Suc Y, Guillamonc JM, Aybara MJ (2020) Growth and lipid production of Rhodotorula glutinis R4, in comparison to other oleaginous yeasts. J Biotechnol 310:21–31
Mishra VK, Goswami R (2017) A review of production, properties and advantages of biodiesel. Biofuels. https://doi.org/10.1080/17597269.2017.1336350
Nilsson A, Gorwa-Grauslund MF, Hahn-Hagerdal B, Liden G (2005) Cofactor dependence in furan reduction by Saccharomyces cerevisiae in fermentation of acid-hydrolyzed lignocellulose. Appl Environ Microbiol 71(12):7866–7871
Ochsenreither K, Glück C, Stressler T, Fischer L, Syldatk C (2016) Production Strategies and Applications of Microbial Single Cell Oils. Front Microbiol 7:1539. https://doi.org/10.3389/fmicb.2016.01539
Parawira W, Tekere M (2011) Biotechnological strategies to overcome inhibitors in lignocellulose hydrolysates for ethanol production: review. Crit Rev Biotechnol 31(1):20–31
Park GW, Chang HN, Jung K, Seob C, Kim YC, Choi JH, Woo HC, Hwang IJ (2017) Production of microbial lipid by Cryptococcus curvatus on rice strawhydrolysates. Process Biochem 56:147–153
Pejin B, Iodice C, Tommonaro G, Sabovljevic M, Bianco A, Tesevic V, Vajs V, De Rosa S (2012) Sugar composition of the moss Rhodobryum ontariense (Kindb.) Kindb. Nat Prod Res. https://doi.org/10.1080/14786419.2010.535163
Ratledge C (2013) Microbial oils: an introductory overview of current status and future prospects. Oilseeds & fats Crops and Lipids (OCL). https://doi.org/10.1051/ocl/2013029
Rocha GJM, Martin C, Soares IB, Maior AMS, Baudel HM, Abreu CAM (2011) Dilute mixed-acid pretreatment of sugarcane bagasse for ethanol production. Biomass Bioenerg 35(1):663–670
Santamauro F, Whiffin FM, Scott RJ, Chuck CJ (2014) Low-cost lipid production by an oleaginous yeast cultured in non-sterile conditions using model waste resources. Biotechonol Biofuels 7:34–34
Silva DDV, Arruda PV, Dussán KJ, Felipe MGA (2014) Adaptation of Scheffersomyces stipitis Cells as a Strategy to the Improvement of Ethanol Production from Sugarcane Bagasse Hemicellulosic Hydrolysate. Chem Eng Trans 38:427–432
Srienc F, Arnold B, Bailey JE (1984) Characterization of intracellular accumulation of poly-beta-hydroxybutyrate (PHB) in individual cells of Alcaligenes eutrophus H16 by flow cytometry. Biotechnol Bioeng 26:982
Subramaniam R, Dufreche S, Zappi M, Bajpai R (2010) Microbial lipids from renewable resources: production and characterization. J Ind Microbiol Biotechnol 37(12):1271–1287
Sutanto S, Zullaikah S, Tran-Nguyen PL, Ismadji S, Jua YH (2018) Lipomyces starkeyi: Its current status as a potential oil producer. Fuel Process Technol 177:39–55
Takaku H, Matsuzawa T, Yaoi K, Yamazaki H (2020) Lipid metabolism of the oleaginous yeast Lipomyces starkeyi. Appl Microbiol Biotechnol. https://doi.org/10.1007/s00253-020-10695-9
Tapia EV, Anschau A, Coradini ALV, Franco TT, Deckmann AC (2012) Optimization of lipid production by the oleaginous yeast Lipomyces starkeyi by random mutagenesis coupled to cerulenin screening. AMB Express 2(64):1–8
Tchakouteu SS, Kalantzi O, Gardeli C, Koutinas AA, Aggelis G, Papanikolaou S (2015) Lipid production by yeasts growing on biodiesel-derived crude glycerol: strain selection and impact of substrate concentration on the fermentation efficiency. J Appl Microbiol 118(4):911–927
Thagaraj B, Solomon PR, Muniyandi B, Ranganathan S, Lin L (2019) Catalysis in biodiesel production—a review. Clean Energy 3(1):2–23
Thanapimmetha A, Peawsuphon N, Chisti Y, Saisriyoot M, Srinophakun P (2019) Lipid production by the yeast Lipomyces starkeyi grown on sugars and oil palm empty fruit bunch hydrolysate. Biomass Convers Bior. https://doi.org/10.1007/s13399-019-00532-z
Tsegaye B, Balomajumder C, Roy P (2019) Microbial delignification and hydrolysis of lignocellulosic biomass to enhance biofuel production: an overview and future prospect. Bull Natl Res Cent 43:5
Tsoutsos T, Bethanis D (2011) Optimization of the Dilute Acid Hydrolyzator for Cellulose-to-Bioethanol Saccharification. Energies 4(12):1601–1623
Vieira JPF, Ienczak JL, Rossell CEV, Pradella JGC, Franco TT (2014) Microbial lipid production: screening with yeasts grown on Brazilian molasses. Biotechnol Lett 36(12):2433–2442
Wang R, Wang J, Xu R, Fang Z, Liu A (2014) Oil production by the oleaginous yeast Lipomyces starkeyi using diverse carbon sources. BioResources 9(4):7027–7040
Westman J, Franzén CJ (2015) Current progress in high cell density yeast bioprocesses for bioethanol production. Biotechnol J 10:1185–1195
Wild R, Patil S, Popovic M, Zappi M, Dufreche S, Baipai R (2010) Lipids from Lipomyces starkeyi. Food Technol Biotechnol 48:329–335
Xavier MCA, Coradini ALV, DeckmannAC FTT (2017) Lipid production from hemicellulose hydrolysate and acetic acid by Lipomyces starkeyi and the ability of yeast to metabolize inhibitors. Biochem Eng J 118:11–19
Xiong L, Huang C, Yang XY, Lin XQ, Chen XF, Wang C, Wang B, Zeng XA, Chen XD (2015) Beneficial effect of corncob acid hydrolysate on the lipid production by oleaginous yeast Trichosporon dermatis. Prep Biochem Biotechnol 45(5):421–429
Zhao X, Kong X, Hua Y, Feng B, Zhao Z (2008) Medium optimization for lipid production through co-fermentation of glucose and xylose by the oleaginous yeast Lipomyces starkeyi. Eur J Lipid Sci Technol 110(5):405–412
Zhao X, Peng F, Du W, Liu C, Liu D (2012) Effects of some inhibitors on the growth and lipid accumulation of oleaginous yeast Rhodosporidium toruloides and preparation of biodiesel by enzymatic transesterification of the lipid. Bioprocess Biosyst Eng 35(6):993–1004
Zhu JJ, Yong Q, Xu Y, Chen SX, Yu SY (2009) Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolysate. Natural Sci 1(1):47–54
We would like to thank FAPESP (Foundation for Research Support of the State of São Paulo), CAPES (Coordination for the Improvement of Higher Education Personnel) and CNPq (National Council for Scientific and Technological Development) for their support of this research. We would like to thank LNBR (Brazilian Biorenewables National Laboratory) and Dr. Carlos Vaz Rossel for providing the sugarcane bagasse and the Parr reactor.
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da Cunha Abreu Xavier, M., Teixeira Franco, T. Obtaining hemicellulosic hydrolysate from sugarcane bagasse for microbial oil production by Lipomyces starkeyi. Biotechnol Lett (2021). https://doi.org/10.1007/s10529-021-03080-7
- Microbial oil
- Lipomyces starkeyi