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Electrospun essential oil-polycaprolactone nanofibers as antibiofilm surfaces against clinical Candida tropicalis isolates

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Abstract

Objective

As an approach to prevent biofilm infections caused by Candida tropicalis on various surfaces, determination of effect of biodegradable polycaprolactone nanofibers (PCLNFs) with different concentrations of two different essential oils were tested in this study.

Results

Both of the tested essential oils exhibited antifungal effect (minimal inhibitory concentration; 0.25–0.49 µL/mL, minimal fungicidal concentration; 0.25–0.49 µL/mL, depending on the C. tropicalis strain) (Zone of inhibition caused by 500 μL/mL concentration of oils; 28–56 mm). 0, 2, 4% clove oil PCLNFs and 0, 2, 4% red thyme oil-PCLNFs were free from bead formation and uniform in diameter. Diameters of all essential oil containing PCLNFs were ranged from 760 to 1100 nm and were significantly different from 0% essential oil-PCLNF (P < 0.05). 0, 2, 4% clove oil-PCLNFs were significantly more hydrophobic compared to 8% clove oil-PCLNF (P < 0.01), whereas 0% and 2% red thyme oil-PCLNFs were significantly more hydrophobic compared to 4% and 8% red thyme oil PCLNFs (P < 0.01). Highest amount of biofilm inhibition was observed by 4% clove oil-PCLNF and by 4% red thyme oil-PCLNF.

Conclusions

Clove and red thyme oils may be used not only as antifungals but also as biofilm inhibitive agents on surfaces of biomaterials that are frequently contaminated by C. tropicalis, when they are incorporated into PCLNFs.

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Acknowledgements

We are grateful to Scientific Research Projects Coordination Unit of Hacettepe University, Ankara, Turkey for supporting this study (Grant No.: FHD-2017-13075) and also to the Scientific and Technological Research Council of Turkey, 1003 project (TUBITAK project No: 113O864) for the production of nanofibers.

Funding

This study was supported by the funding received from Scientific Research Projects Coordination Unit (grant number: FHD-2017-13075) of Hacettepe University, Ankara, Turkey. Additionally, production of nanofibers was supported by the Scientific and Technological Research Council of Turkey, 1003 project (TUBITAK project No: 113O864) and Behzad Nasseri and Aliakbar Ebrahimi were also supported by the mentioned project.

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Authors and Affiliations

  1. Biotechnology Division, Biology Department, Faculty of Sciences, Hacettepe University, 06800, Beytepe, Ankara, Turkey

    Gulcan Sahal & Isil Seyis Bilkay

  2. Chemical Engineering and Applied Chemistry Department, Atilim University, 06830, Ankara, Turkey

    Behzad Nasseri

  3. Chemical Engineering Department and Bioengineering Division, Hacettepe University, 06800, Beytepe, Ankara, Turkey

    Behzad Nasseri

  4. Nanotechnology and Nanomedicine Division, Hacettepe University, 06800, Beytepe, Ankara, Turkey

    Aliakbar Ebrahimi

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  1. Gulcan Sahal
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  2. Behzad Nasseri
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  4. Isil Seyis Bilkay
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Correspondence to Gulcan Sahal.

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Sahal, G., Nasseri, B., Ebrahimi, A. et al. Electrospun essential oil-polycaprolactone nanofibers as antibiofilm surfaces against clinical Candida tropicalis isolates. Biotechnol Lett 41, 511–522 (2019). https://doi.org/10.1007/s10529-019-02660-y

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