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Conditioned medium from umbilical cord mesenchymal stem cells improves nasal mucosa damage by radiation

Abstract

Objectives

To explore therapeutic effects of conditioned medium from human umbilical cord mesenchymal stem cells (hUC-MSCs) on nasal mucosa radiation damage both in vivo and in vitro.

Results

The mucus cilia clearance time (7 and 30 days), degree of mucosal edema (7, 30, 90 and 180 days), cilia coverage (180 days) of concentrated conditioned medium group improved compared with radiotherapy control group. The proliferation and migration abilities of irradiated and non-irradiated nasal epithelial cells significantly increased after culture in bronchial epithelial cell growth medium (BEGM) containing 10% conditioned medium of hUC-MSCs compared to cells cultured in BEGM alone.

Conclusions

Soluble factors secreted by hUC-MSCs may promote nasal epithelial cell proliferation and migration. Intranasal administration of hUC-MSC conditioned medium effectively repairs nasal mucosa radiation damage.

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Acknowledgements

This study was supported by Medicine and Health General Research Program of Zhejiang Province, No. 2016155085.

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Correspondence to Fang Ji.

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The authors declare no conflicts of interest.

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Duan, HG., Ji, F., Zheng, CQ. et al. Conditioned medium from umbilical cord mesenchymal stem cells improves nasal mucosa damage by radiation. Biotechnol Lett 40, 999–1007 (2018). https://doi.org/10.1007/s10529-018-2553-0

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  • DOI: https://doi.org/10.1007/s10529-018-2553-0

Keywords

  • Cytokines
  • Conditioned medium
  • Mesenchymal stem cells
  • Nasal mucosa
  • Radiation damage