Establishment of dermal sheath cell line from Cashmere goat and characterizing cytokeratin 13 as its novel biomarker
To establish a dermal sheath cell line, a dermal papilla cell line and a outer root sheath cell line from Cashmere goat and clarify the similarities and differences among them.
We established a dermal sheath cell line, a dermal papilla cell line and a outer root sheath cell line from the pelage skin hair follicles of Cashmere goat. The growth rate of dermal sheath cells was intermediate between that of dermal papilla cells and outer root sheath cells. Immunofluorescence experiments and reverse transcription-polymerase chain reaction analysis showed that at both the transcriptional and translational levels, the dermal sheath cells were alpha-smooth muscle actin (α-SMA)+/cytokeratin 13+, while the dermal papilla cells were α-SMA+/cytokeratin 13- and the outer root sheath cells were α-SMA-/cytokeratin 13+. Patterns of cytokeratin 13 expression could distinguish the dermal sheath cells from the dermal papilla cells.
These results suggest that cytokeratin 13 could serve as a novel biomarker for dermal sheath cells of Cashmere goat, and should prove useful for researchers investigating dermal stem cells or interaction of different types of cells during hair cycle.
KeywordsBiomarker Cytokeratin 13 Dermal papilla cell Dermal sheath cell Hair follicle Outer root sheath cell
This work was supported by the Inner Mongolia Natural Science Foundation of China (Grant No. 2016BS0802, 2015BS0805), the Projects of Medical Scientific Research of Health & Family Planning Commission of Inner Mongolia (Grant No. 201703183), the Projects of Medical Scientific Research of Health & Family Planning Commission of Baotou (Grant No. WSJJ2016060), the Health Research Projects of Metallurgical Safety and Health Branch of Chinese Society for Metals (Grant No. JKWS201639), and the Science & Technology Project of Quality Control of China (Grant No. 2015IK176).
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Conflict of interest
We declare that we have no conflicts of interest.
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