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Recent advances in enhanced enzyme activity, thermostability and secretion by N-glycosylation regulation in yeast

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Abstract

Yeast has been increasingly used as a host for the expression of enzymes. Compared to other expression systems, the yeast expression system has many advantages including its suitability for large-scale fermentation and its ability to modify enzymes. When expressed in yeast, many recombinant enzymes are N-glycosylated, and this may play an important role in their activity, thermostability and secretion. Although the mechanism underlying this process is not clear, the regulation of N-glycosylation by introducing or eliminating N-glycosylation at specific sites has developed into an important strategy for improving the production or catalytic properties of recombinant enzymes. In this review, we summarize the recent advances in understanding the effects of N-glycosylation on the expression and characteristics of recombinant enzymes, and discuss novel strategies for regulating N-glycosylation in yeast. We hope that this review will help improve the understanding of the expression and the catalytic properties of N-glycosylated proteins.

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Acknowledgements

This work is financially supported by National natural science foundation of China (31671797); Natural sciences foundation supported by Anhui Province universities (KJ2016A801) and the Anhui polytechnic university youth talent support program (2016BJRC006).

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Author notes

  1. Longbao Zhu have contributed equally to this work.

Authors and Affiliations

  1. School of Biochemical Engineering, Anhui Polytechnic University, 8 Zheshan Road, Wuhu, 241000, Anhui, People’s Republic of China

    Fei Ge, Longbao Zhu, Anna Aang, Ping Song, Wanzhen Li & Yugui Tao

  2. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Guocheng Du

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  1. Fei Ge
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  2. Longbao Zhu
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  3. Anna Aang
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  4. Ping Song
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  5. Wanzhen Li
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  6. Yugui Tao
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  7. Guocheng Du
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Correspondence to Yugui Tao.

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Ge, F., Zhu, L., Aang, A. et al. Recent advances in enhanced enzyme activity, thermostability and secretion by N-glycosylation regulation in yeast. Biotechnol Lett 40, 847–854 (2018). https://doi.org/10.1007/s10529-018-2526-3

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  • DOI: https://doi.org/10.1007/s10529-018-2526-3

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